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Hypothesized and experimentally tested mechanisms for PRTx dethreading. (A) Glutathione incubation with PRTx at acidic and neutral pH and (B) acid catalyzed dethreading at 37 °C showed no signs of dethreading. Hypothesized dethreading (C) by enzymatic cleavage of carbamate linkages yields free β-CD in vitro.
