Abstract
Listeria monocytogenes is a facultative intracellular foodborne pathogen that can cause listeriosis in humans and animals. L. monocytogenes LM201 was isolated from foodstuff. The draft genome sequence of strain LM201 provides the genetic basis for the application of this strain in biotechnological vaccine production.
GENOME ANNOUNCEMENT
Listeria monocytogenes is a Gram-positive, nonsporulating, aerobic and facultative anaerobic, rod-shaped, and facultative intracellular bacterium (1, 2). It is a foodborne pathogen (3) that can cause listeriosis (gastroenteritis, meningitis, encephalitis, septicemia, and abortion) in animals and humans, especially immunocompromised individuals (the old, neonates, and pregnant women), with a mean mortality rate of 20–30% or higher (4). L. monocytogenes LM201, a serovar 4b strain, was isolated from foodstuff in Hubei province (China). The LD50 of strain LM201 was 6.3 × 106 CFU for Kunming mice after i.p. infection. Strain LM201 was also assayed for other phenotypic characteristics and was determined to be a good candidate for developing a vaccine. Here we report the genome sequence of L. monocytogenes LM201, which provides the genetic basis for rationally designing and generating defined virulence-attenuated strains for biotechnological vaccine production.
Genomic DNA of the strain LM201 was sequenced with a whole-genome shotgun strategy using an Illumina HiSeq 2000 platform. Two different sequencing libraries (300-bp paired end and 5,000-bp mate pair) were prepared. In total, 43,930,390 paired-end reads of 100 bp and 20,921,890 mate-pair reads of 90 bp were obtained from the sequencing (1,910-fold genome coverage). De novo assembly was done using ABySS version 1.3.4 software (5), yielding 86 contigs with an N50 size of 226,587 bp and a maximum contig size of 342,356 bp. A total of 66 scaffolds were produced, with a total size of 3,200,729 bp and a G+C content of 37.44%.
Genome annotation was accomplished using the RAST server (6). Gene prediction revealed 3,165 protein-coding genes (CDSs), 6 rRNA operons, and 35 tRNA genes. Among the protein-coding genes, there were 2,736 genes with function prediction; 2,504 were identified in the COG database; and 2,674 were identified in the Pfam database.
Nucleotide sequence accession number.
The L. monocytogenes LM201 draft genome sequence has been deposited in the GenBank database under the accession number AYPT00000000. The version described in this paper is the first version.
ACKNOWLEDGMENTS
This work was supported by grants from the National High Technology Research and Development Program (863) of China (2011AA10A203) and the China 948 Program of the Ministry of Agriculture (G25).
Footnotes
Citation Wu Y, Zheng J, Wang Y, Li S, Jin H, Li Z, Bi D, Sun M, Liu M. 2015. Draft genome sequence of Listeria monocytogenes LM201, isolated from foodstuff. Genome Announc 3(1):e01417-14. doi:10.1128/genomeA.01417-14.
REFERENCES
- 1.Seeliger HPR, Jones D. 1986. Genus Listeria, p 1235–1245. In Sneath PHA, Mair NS, Sharpe ME, Holt JG (ed), Bergey’s manual of systematic bacteriology, vol 2 Williams and Wilkins, Baltimore, MD. [Google Scholar]
- 2.Freitag NE, Port GC, Miner MD. 2009. Listeria monocytogenes—from saprophyte to intracellular pathogen. Nat Rev Microbiol 7:623–628. doi: 10.1038/nrmicro2171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3.Farber JM, Peterkin PI. 1991. Listeria monocytogenes, a foodborne pathogen. Microbiol Rev 55:476–511. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4.Vázquez-Boland JA, Kuhn M, Berche P, Chakraborty T, Domínguez-Bernal G, Goebel W, González-Zorn B, Wehland J, Kreft J. 2001. Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev 14:584–640. doi: 10.1128/CMR.14.3.584-640.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5.Simpson JT, Wong K, Jackman SD, Schein JE, Jones SJM, Birol İ. 2009. ABySS: a parallel assembler for short read sequence data. Genome Res 19:1117–1123. doi: 10.1101/gr.089532.108. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6.Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, Meyer F, Olsen GJ, Olson R, Osterman AL, Overbeek RA, McNeil LK, Paarmann D, Paczian T, Parrello B, Pusch GD, Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O. 2008. The RAST server: Rapid Annotations using Subsystems Technology. BMC Genomics 9:75. doi: 10.1186/1471-2164-9-75. [DOI] [PMC free article] [PubMed] [Google Scholar]