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. 1977 Oct;74(10):4462–4465. doi: 10.1073/pnas.74.10.4462

MRNA-directed synthesis of catalytically active mouse beta-glucuronidase in Xenopus oocytes.

C Labarca, K Paigen
PMCID: PMC431963  PMID: 270691

Abstract

Catalytically active mouse beta-glucuronidase (beta-D-glucuronide glucuronosohydrolase, EC 3.2.1.31) is formed when Xenopus oocytes are injected with mouse RNA enriched for poly(A)-containing mRNA sequences. With the RNA from androgen-induced kidneys, the efficiency of translation is comparable to that of endogenous Xenopus messenger, and the fidelity of translation is high. Detection of glucuronidase messenger by formation of a catalytically active product is several orders of magnitude more sensitive than detection by incorporation of isotopically labeled amino acids. As well as providing a sensitive technique for examining the regulation of gene expression, the system makes available an opportunity to study the regulation of post-translational polypeptide processing of a lysosomal enzyme.

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Selected References

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