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. 1977 Oct;74(10):4610–4614. doi: 10.1073/pnas.74.10.4610

Activation of cytotoxic T cells by nonstimulating tumor cells and spleen cell factor(s).

D W Talmage, J A Woolnough, H Hemmingsen, L Lopez, K J Lafferty
PMCID: PMC431996  PMID: 303772

Abstract

The ability of three cultured mouse tumor lines to stimulate a cytotoxic response in 5-day cultures of allogeneic lymph node cells was studied with a 51Cr release assay. Two lines of mesenchymal origin, P815 and EL-4, were found to be highly stimulatory, whereas the third cell line, CaD2, a mammary gland epithelial tumor, did not stimulate over a wide range of cell concentration. CaD2 cells were shown to contain major antigens similar to those of P815 cells by the specific lysis of both cells by lymphocytes activated to H-2d-bearing peritoneal cells.UV-irradiated P815-cells, like gamma-irradiated CaD2 cells, did not stimulate a cytotoxic response, but both cell lines were found to stimulate a full and specific response to allogeneic lymph node cells if these mixed cultures were supplemented with a supernatant harvested from concanavalin A-stimulated spleen cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askonas B. A., Schimpl A., Wecker E. The differentiation function of T cell-replacing factor in nu-nu spleen cell cultures. Eur J Immunol. 1974 Mar;4(3):164–169. doi: 10.1002/eji.1830040304. [DOI] [PubMed] [Google Scholar]
  2. Bach F. H., Bach M. L., Sondel P. M. Differential function of major histocompatibility complex antigens in T-lymphocyte activation. Nature. 1976 Jan 29;259(5541):273–281. doi: 10.1038/259273a0. [DOI] [PubMed] [Google Scholar]
  3. Cantor H., Boyse E. A. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med. 1975 Jun 1;141(6):1390–1399. doi: 10.1084/jem.141.6.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Erb P., Feldmann M. The role of macrophages in the generation of T helper cells. III. Influence of macrophage-derived factors in helper cell induction. Eur J Immunol. 1975 Nov;5(11):759–766. doi: 10.1002/eji.1830051106. [DOI] [PubMed] [Google Scholar]
  5. Frelinger J. A., Neiderhuber J. E., David C. S., Shreffler D. C. Evidence for the expression of Ia (H-2-associated) antigens on thymus-derived lymphocytes. J Exp Med. 1974 Nov 1;140(5):1273–1284. doi: 10.1084/jem.140.5.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gery I., Waksman B. H. Potentiation of the T-lymphocyte response to mitogens. II. The cellular source of potentiating mediator(s). J Exp Med. 1972 Jul 1;136(1):143–155. doi: 10.1084/jem.136.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Greineder D. K., Rosenthal A. S. Macrophage activation of allogeneic lymphocyte proliferation in the guinea pig mixed leukocyte culture. J Immunol. 1975 May;114(5):1541–1547. [PubMed] [Google Scholar]
  8. Kung J. T., Brooks S. B., Jakway J. P., Leonard L. L., Talmage D. W. Suppression of in vitro cytotoxic response by macrophages due to induced arginase. J Exp Med. 1977 Sep 1;146(3):665–672. doi: 10.1084/jem.146.3.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lafferty K. J., Bootes A., Dart G., Talmage D. W. Effect of organ culture on the survival of thyroid allografts in mice. Transplantation. 1976 Aug;22(2):138–149. doi: 10.1097/00007890-197608000-00009. [DOI] [PubMed] [Google Scholar]
  10. Lafferty K. J., Cunningham A. J. A new analysis of allogeneic interactions. Aust J Exp Biol Med Sci. 1975 Feb;53(1):27–42. doi: 10.1038/icb.1975.3. [DOI] [PubMed] [Google Scholar]
  11. Lafferty K. J., Misko I. S., Cooley M. A. Allogeneic stimulation modulates the in vitro response of T cells to transplantation antigen. Nature. 1974 May 17;249(454):275–276. doi: 10.1038/249275a0. [DOI] [PubMed] [Google Scholar]
  12. Lafferty K. J., Talmage D. W. Theory of allogeneic reactivity and its relevance to transplantation biology. Transplant Proc. 1976 Sep;8(3):349–353. [PubMed] [Google Scholar]
  13. Lightbody J. J., Kong Y. M. Mitogen-stimulated glutaraldehyde-fixed spleen cells: ability to stimulate in the mixed lymphocyte reaction and generate effector cells in cell-mediated lympholysis. J Immunol. 1976 Oct;117(4):1336–1339. [PubMed] [Google Scholar]
  14. Paetkau V., Mills G., Gerhart S., Monticone V. Proliferation of murine thymic lymphocytes in vitro is mediated by the concanavalin A-induced release of a lymphokine (costimulator). J Immunol. 1976 Oct;117(4):1320–1324. [PubMed] [Google Scholar]
  15. Pick E., Kotkes P. A simple method for the production of migration inhibitory factor by concanavalin A - stimulated lymphocytes. J Immunol Methods. 1977;14(2):141–146. doi: 10.1016/0022-1759(77)90004-7. [DOI] [PubMed] [Google Scholar]
  16. Talmage D. W., Hemmingsen H. Is the macrophage the stimulating cell? J Allergy Clin Immunol. 1975 Jun;55(6):442–450. doi: 10.1016/0091-6749(75)90083-4. [DOI] [PubMed] [Google Scholar]

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