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. 1977 Nov;74(11):4891–4894. doi: 10.1073/pnas.74.11.4891

Ribonuclease III cleavage of bacteriophage T3RNA polymerase transcripts to late T3 mRNAs.

H K Majumder, S Bishayee, P R Chakraborty, U Maitra
PMCID: PMC432062  PMID: 337303

Abstract

In vitro transcription of T3 DNA by T3 phage-induced RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6) yields eight discrete RNAs (designated I-VIII) with molecular weights of approximately 6.2, 4.7, 4, 2.8, 1.8, 0.9, 0.52, and 0.21 X 10(6), respectively. Comparison of the size of in vitro T3 RNA polymerase transcripts with in vivo late T3 mRNAs indicates that several late RNAs produced in T3-infected cells do not correspond to any of the in vitro RNAs, and no RNAs as large as the three largest in vitro RNA species, I, II, and III, are observed. Escherichia coli RNase III cleaves these three high molecular weight T3 RNA polymerase transcripts to discrete RNAs that comigrate in polyacrylamide gel electrophoresis with some of the late T3 RNAs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beier H., Hausmann R. Genetic map of bacteriophage T3. J Virol. 1973 Aug;12(2):417–419. doi: 10.1128/jvi.12.2.417-419.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bishayee S., Maitra U. Specificity of cleavage by ribonuclease III. Biochem Biophys Res Commun. 1976 Nov 22;73(2):306–313. doi: 10.1016/0006-291x(76)90708-7. [DOI] [PubMed] [Google Scholar]
  3. Chakraborty P. R., Bandyopadhyay P., Huang H. H., Maitra U. Fidelity of in vitro transcription of T3 deoxyribonucleic acid by bacteriophage T3-induced ribonucleic acid polymerase and by Escherichia coli ribonucleic acid polymerase. J Biol Chem. 1974 Nov 10;249(21):6901–6909. [PubMed] [Google Scholar]
  4. Chakraborty P. R., Salvo R. A., Majumder H. K., Maitra U. Further characterization of bacteriophage T3-induced ribonucleic acid polymerase. Studies on the size of in vitro transcripts and interaction of T3 RNA polymerase with T3 DNA. J Biol Chem. 1977 Sep 25;252(18):6485–6493. [PubMed] [Google Scholar]
  5. Chakraborty P. R., Sarkar P., Huang H. H., Maitra U. Studies on T3-induced ribonucleic acid polymerase. 3. Purification and characterization of the T3-induced ribonucleic acid polymerase from bacteriophage T3-infected Escherichia coli cells. J Biol Chem. 1973 Oct 10;248(19):6637–6646. [PubMed] [Google Scholar]
  6. Dunn J. J., Bautz F. A., Bautz E. K. Different template specificities of phage T3 and T7 RNA polymerases. Nat New Biol. 1971 Mar 17;230(11):94–96. doi: 10.1038/newbio230094a0. [DOI] [PubMed] [Google Scholar]
  7. Dunn J. J., McAllister W. T., Bautz E. K. In vitro transcription of T3 DNA by Escherichia coli and T3 polymerases. Virology. 1972 Apr;48(1):112–125. doi: 10.1016/0042-6822(72)90119-5. [DOI] [PubMed] [Google Scholar]
  8. Dunn J. J., Studier F. W. T7 early RNAs and Escherichia coli ribosomal RNAs are cut from large precursor RNAs in vivo by ribonuclease 3. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3296–3300. doi: 10.1073/pnas.70.12.3296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dunn J. J., Studier F. W. T7 early RNAs are generated by site-specific cleavages. Proc Natl Acad Sci U S A. 1973 May;70(5):1559–1563. doi: 10.1073/pnas.70.5.1559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Floyd R. W., Stone M. P., Joklik W. K. Separation of single-stranded ribonucleic acids by acrylamide-agarose-urea gel electrophoresis. Anal Biochem. 1974 Jun;59(2):599–609. doi: 10.1016/0003-2697(74)90313-3. [DOI] [PubMed] [Google Scholar]
  11. Golomb M., Chamberlin M. J. T7- and T3-specific RNA polymerases: characterization and mapping of the in vitro transcripts read from T3 DNA. J Virol. 1977 Feb;21(2):743–752. doi: 10.1128/jvi.21.2.743-752.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Golomb M., Chamberlin M. A preliminary map of the major transcription units read by T7 RNA polymerase on the T7 and T3 bacteriophage chromosomes. Proc Natl Acad Sci U S A. 1974 Mar;71(3):760–764. doi: 10.1073/pnas.71.3.760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maitra U., Huang H. H. Initiation, release, and reinitiation of RNA chains by bacteriophage-T3-induced polymerase from T3 DNA templates (E. coli-guanosine triphosphate terminus-purified polymerase). Proc Natl Acad Sci U S A. 1972 Jan;69(1):55–59. doi: 10.1073/pnas.69.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maitra U. Induction of a new RNA polymerase in Escherichia coli infected with bacteriophage T3. Biochem Biophys Res Commun. 1971 Apr 16;43(2):443–450. doi: 10.1016/0006-291x(71)90773-x. [DOI] [PubMed] [Google Scholar]
  15. Maitra U., Salvo R. A., Chakraborty P. R. Specificity of ribonucleic acid chain initiation by bacteriophage T3-induced ribonucleic acid polymerase. J Biol Chem. 1974 Sep 25;249(18):5835–5839. [PubMed] [Google Scholar]
  16. Peacock A. C., Dingman C. W. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. doi: 10.1021/bi00842a023. [DOI] [PubMed] [Google Scholar]
  17. Robertson H. D., Webster R. E., Zinder N. D. Purification and properties of ribonuclease III from Escherichia coli. J Biol Chem. 1968 Jan 10;243(1):82–91. [PubMed] [Google Scholar]
  18. Salvo R. A., Chakraborty P. R., Maitra U. Studies on T3-induced ribonucleic acid polymerase. IV. Transcription of denatured deoxyribonucleic acid preparations by T3 ribonucleic acid polymerase. J Biol Chem. 1973 Oct 10;248(19):6647–6654. [PubMed] [Google Scholar]

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