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. 2013 Dec 23;8(1-2):153–161. doi: 10.1002/jbio.201300159

Qualitative tissue differentiation by analysing the intensity ratios of atomic emission lines using laser induced breakdown spectroscopy (LIBS): prospects for a feedback mechanism for surgical laser systems

Rajesh Kanawade 1,2,*, Fanuel Mahari 1,2,*, Florian Klämpfl 1,2, Maximilian Rohde 3, Christian Knipfer 3, Katja Tangermann-Gerk 4, Werner Adler 5, Michael Schmidt 1,2,4, Florian Stelzle 1,3
PMCID: PMC4320783  PMID: 24376030

Abstract

The research work presented in this paper focuses on qualitative tissue differentiation by monitoring the intensity ratios of atomic emissions using ‘Laser Induced Breakdown Spectroscopy’ (LIBS) on the plasma plume created during laser tissue ablation. The background of this study is to establish a real time feedback control mechanism for clinical laser surgery systems during the laser ablation process. Ex-vivo domestic pig tissue samples (muscle, fat, nerve and skin) were used in this experiment. Atomic emission intensity ratios were analyzed to find a characteristic spectral line for each tissue. The results showed characteristic elemental emission intensity ratios for the respective tissues. The spectral lines and intensity ratios of these specific elements varied among the different tissue types. The main goal of this study is to qualitatively and precisely identify different tissue types for tissue specific laser surgery. (© 2013 WILEY-VCH Verlag GmbH &Co. KGaA, Weinheim)

Keywords: Laser induced breakdown spectroscopy, plasma, chemical composition, chemical elements, tissue type, intensity ratio

Supporting Information

Author biographies Please see Supporting Information online.

jbio0008-0153-sd1.pdf (1.5MB, pdf)

References

  • 1.Stelzle F, Zam A, Adler W, K. Tangermann-Gerk ADouplik, Nkenke E, Schmidt M. J. Translational Medicine. 2011;9:20. doi: 10.1186/1479-5876-9-20. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Stelzle F, Terwey I, Knipfer C, Adler W, K. Tangermann-Gerk ENkenke, Schmidt M. J. Translational Medicine. 2012;10:123. doi: 10.1186/1479-5876-10-123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Stelzle F, K. Tangermann-Gerk WAdler, Zam A, Schmidt M, Douplik A, Nkenke W. Lasers in Surgery and Medicine. 2010;42:319–325. doi: 10.1002/lsm.20909. [DOI] [PubMed] [Google Scholar]
  • 4.Baxter G, Walsh D, Allen J, Lowe A, Bell A. Experimental Physiology. 1994;79:227–234. doi: 10.1113/expphysiol.1994.sp003755. [DOI] [PubMed] [Google Scholar]
  • 5.Menovsky T, van M. den Bergh Weerman, and J. F. Beek, Microsurgery. 1996;17:562–567. doi: 10.1002/(SICI)1098-2752(1996)17:10<562::AID-MICR6>3.0.CO;2-S. [DOI] [PubMed] [Google Scholar]
  • 6.Kanawade R, Mehari F, Knipfer C, Rohde M, K. Tangermann-Gerk MSchmidt, Stelzle F. Spectrochimica Acta Part B. 2013;87:175–181. [Google Scholar]
  • 7.Ulyanov VA, Gordienko VM, Dmitriev AK, Kortunov VN, Panchenko VY, Poutivski IY, Phischyuk YA. SPIE Proceedings. 1998;3195:88–93. [Google Scholar]
  • 8.Boppart SA, Herrmann J, Pitris C, Stamper DL, Brezinski ME, Fujimoto JG. J. of Surgical Research. 1999;82:275–284. doi: 10.1006/jsre.1998.5555. [DOI] [PubMed] [Google Scholar]
  • 9.Eberhard J, Eisenbeiss AK, Braun A, J. Hedderich, and SJepsen. Caries Research. 2005;39:496–504. doi: 10.1159/000088186. [DOI] [PubMed] [Google Scholar]
  • 10.Tangermann K, Roth S, Muller D, Tragler H, Uller J, Rupprecht S. Laser Florence A Window on the Laser Medicine World. Proc. SPIE. 2003;5287:24–34. and 5287. [Google Scholar]
  • 11.Rupprecht S, K. Tangermann-Gerk JWiltfang, Neukam F, Schlegel A. Lasers in Medical Science. 2004;19:81–88. doi: 10.1007/s10103-004-0301-2. [DOI] [PubMed] [Google Scholar]
  • 12.Rupprecht S, K. Tangermann-Gerk, S. Schultze-Mosgau FWNeukam, Ellrich J. Lasers in Surgery and Medicine. 2005;36:186–192. doi: 10.1002/lsm.20133. [DOI] [PubMed] [Google Scholar]
  • 13.Singh JP, Almirall J, Sabsabi M, Miziolek AW. Appl Opt. 2012;51:7. doi: 10.1364/AO.51.0LIBS1. [DOI] [PubMed] [Google Scholar]
  • 14.Singh V, Rai A. Lasers Med. Sci. 2011;26:673–687. doi: 10.1007/s10103-011-0921-2. [DOI] [PubMed] [Google Scholar]
  • 15.Samek O, Telle H, Beddows D. BMC Oral Health. 2001;1:1. doi: 10.1186/1472-6831-1-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Myakalwar AK, Sreedhar S, Barman I, Dingari NC, Venugopal S, Rao, P. Prem Kiran SPTewari, Manoj G, Kumar Talanta. 2011;87:53–59. doi: 10.1016/j.talanta.2011.09.040. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Shen XK, Ling H, Lu YF. Laser-based Micro and Nanopackaging, and Assembly III SPIE. 2009;7202:72020–72011. [Google Scholar]
  • 18.Kumar A, Yueh FY, Singh JP, Burgess S. Appl. Opt. 2004;43:5399–5403. doi: 10.1364/ao.43.005399. [DOI] [PubMed] [Google Scholar]
  • 19.Rehse S, Salimnia H, Miziolek A. J. Medi. Eng. &Tech. 2012;36:77–89. doi: 10.3109/03091902.2011.645946. [DOI] [PubMed] [Google Scholar]
  • 20.Becker JS, Matusch A, Palm C, Salber D, Morton KA, Becker JS. 2009. and, Metallomics. 2, 104–111.
  • 21.Kaiser J, Novotn K, Martin MZ, Hrdlièka A, Malina R, Hartl M, Adam V, Kizek R. Surface Science Reports. 2012;67:233–243. [Google Scholar]
  • 22.Cremers DA, Radziemski LJ. Handbook of Laser-Induced Breakdown Spectroscopy (Wiley, 2013)
  • 23.Hammer DX, Jansen ED, Frenz M, Noojin GD, Thomas RJ, Noack J, Vogel A, Rockwell BA, Welch AJ, Hammer 1997. Appl Opt. 36, 5630–5640. [DOI] [PubMed]
  • 24.Martin MZ, Stewart AJ, Gwinn KD, Waller JC. Appl Opti. 2010;49:161–167. [Google Scholar]
  • 25.Dingari NC, Barman I, Myakalwar AK, Tewari SP, Kumar M. Ana Chem. 2012;84:2686–2694. doi: 10.1021/ac202755e. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 26.Vogel A, Venugopalan V. Chem Reviews. 2003;103:577–644. doi: 10.1021/cr010379n. [DOI] [PubMed] [Google Scholar]
  • 27.Woodard H, White D. British journal of radiology. 1986;59:1209–1218. doi: 10.1259/0007-1285-59-708-1209. [DOI] [PubMed] [Google Scholar]
  • 28.Bogaerts A, Chen Z, Gijbels R, Vertes A. Spectrochimica Acta Part B. 2003;58:1867–1893. [Google Scholar]
  • 29.Kramida A, Ralchenko Y, Reader J. 2012. NIST Atomic Spectra Database (ver. 5.0), National Institute of Standards and Technology, Gaithersburg, MD (Available at: http://physics.nist.gov/asd)
  • 30.Borg TK, Caulfield JB. Tissue and Cell. 1980;12:197–207. doi: 10.1016/0040-8166(80)90061-0. [DOI] [PubMed] [Google Scholar]
  • 31.Ross MH, Pawlina Wojciech. Histology: A Text and Atlas: with Correlated Cell and Molecular Biology (Baltimore, MD: Lippincott Wiliams &Wilkins, 2006)
  • 32.Singh JP, Thakur SN. Laser-induced breakdown spectroscopy (Elsevier Science, 2007)

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Supplementary Materials

Author biographies Please see Supporting Information online.

jbio0008-0153-sd1.pdf (1.5MB, pdf)

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