Table 3.

ITS-2 sequence pair distance analysis of Dirofilaria immitis isolates compared with homologous isolates (slow/accurate, IUB).

		Percent identity
		1	2	3	4	5	6	7	8	9	10	11	12	13	14
Divergence	1		99.8	94.1	91.0	90.2	90.2	84.7	93.4	95.5	95.7	95.9	95.4	94.5	98.9	1	JX866681.1 Guwahati
	2	0.2		94.1	91.0	90.2	90.2	84.7	93.4	95.5	95.4	95.7	95.2	94.3	98.6	2	JX481279.1 Guwahati
	3	5.1	5.1		95.7	94.9	94.1	91.4	93.3	93.7	95.7	88.6	93.7	86.3	93.7	3	FJ2634568 Brazil
	4	8.7	8.7	4.6		97.6	93.7	89.0	90.2	90.6	92.2	85.5	90.6	83.1	90.6	4	FJ263462 Brazil
	5	9.6	9.6	5.5	2.5		95.3	88.3	90.2	89.8	91.4	84.8	89.8	82.4	89.8	5	FJ263464 Brazil
	6	9.6	9.6	6.3	6.8	5.0		87.9	89.8	89.5	91.4	84.8	89.5	83.2	89.8	6	HM126607 Turkey
	7	13.6	13.6	6.1	8.9	9.3	9.8		88.5	88.5	86.8	80.0	88.1	78.6	84.4	7	JN084168 Iran
	8	4.1	4.1	4.3	7.9	7.4	7.9	12.8		97.4	95.4	89.3	97.1	87.6	93.4	8	JN084166 Iran
	9	1.8	1.8	3.8	7.4	7.9	8.4	12.7	2.8		98.0	91.6	99.2	89.9	95.5	9	JX889634 Iran
	10	0.5	0.7	2.9	6.9	7.3	7.3	13.6	4.1	1.2		92.0	97.0	90.7	95.0	10	EU087699 Mizoram
	11	0.0	0.2	5.4	9.3	10.2	10.2	14.4	4.3	1.9	0.5		93.2	95.4	94.8	11	EU182329 China
	12	1.2	1.5	3.8	7.4	7.9	8.4	13.2	3.1	0.9	0.7	1.3		91.8	94.8	12	EU182331 China
	13	0.7	1.0	6.7	10.5	11.4	10.4	15.5	5.2	2.8	1.3	0.7	2.0		93.4	13	EU182330 China
	14	0.0	0.2	5.1	8.7	9.6	9.6	13.7	4.1	1.8	0.5	0.0	1.2	0.7		14	AF217800 Taiwan
		1	2	3	4	5	6	7	8	9	10	11	12	13	14