Abstract
Antisera were prepared against a clonal line (PC12) of rat pheochromocytoma cells that were grown in either the presence or the absence of nerve growth factor (NGF). PC12 cells respond to NGF by growing neurites and express many differentiated properties of adrenal chromaffin cells and sympathetic neurons. The antisera, after absorption with rat liver, kidney, spleen, and thymus, reacted in the micro-complement fixation and indirect immunofluorescence assays with PC12 cells (both NGF-treated and untreated), brain, adrenal medulla, and superior cervical ganglia, but not with a variety of other tissues. Cross-absorption of the antisera with brain and adrenal medulla indicated the presence of components that were specific to brain, adrenal medulla, and PC12 cells. Brain-specific and adrenal-medulla-specific antigens were also detected in other species, such as rabbit, cat, and mouse. No differences could be detected in the specificity of antisera raised against either NGF-treated or untreated PC12 cells. Indirect immunofluorescence on live cells also detected cell surface antigens on both cell bodies and processes. These results suggest that such antisera may be used to detect, localize, and identify specific neural and adrenal medullary antigens.
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Selected References
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- Akeson R., Herschman H. R. Modulation of cell-surface antigens of a murine neuroblastoma. Proc Natl Acad Sci U S A. 1974 Jan;71(1):187–191. doi: 10.1073/pnas.71.1.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bocchini V., Angeletti P. U. The nerve growth factor: purification as a 30,000-molecular-weight protein. Proc Natl Acad Sci U S A. 1969 Oct;64(2):787–794. doi: 10.1073/pnas.64.2.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bock E., Mellerup E. T., Rafaelsen O. J. Antigen-antibody crossed electrophoresis of water-soluble rat brain antigens. J Neurochem. 1971 Dec;18(12):2435–2440. doi: 10.1111/j.1471-4159.1971.tb00199.x. [DOI] [PubMed] [Google Scholar]
- Dichter M. A., Tischler A. S., Greene L. A. Nerve growth factor-induced increase in electrical excitability and acetylcholine sensitivity of a rat pheochromocytoma cell line. Nature. 1977 Aug 11;268(5620):501–504. doi: 10.1038/268501a0. [DOI] [PubMed] [Google Scholar]
- Fields K. L., Gosling C., Megson M., Stern P. L. New cell surface antigens in rat defined by tumors of the nervous system. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1296–1300. doi: 10.1073/pnas.72.4.1296. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greene L. A., Rein G. Release, storage and uptake of catecholamines by a clonal cell line of nerve growth factor (NGF) responsive pheo-chromocytoma cells. Brain Res. 1977 Jul 1;129(2):247–263. doi: 10.1016/0006-8993(77)90005-1. [DOI] [PubMed] [Google Scholar]
- Greene L. A., Tischler A. S. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424–2428. doi: 10.1073/pnas.73.7.2424. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Humphreys R. E., McCune J. M., Chess L., Herrman H. C., Malenka D. J., Mann D. L., Parham P., Schlossman S. F., Strominger J. L. Isolation and immunologic characterization of a human. B-lymphocyte-specific, cell surface antigen. J Exp Med. 1976 Jul 1;144(1):98–112. doi: 10.1084/jem.144.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Levi-Montalcini R., Angeletti P. U. Nerve growth factor. Physiol Rev. 1968 Jul;48(3):534–569. doi: 10.1152/physrev.1968.48.3.534. [DOI] [PubMed] [Google Scholar]
- Martin S. E. Mouse brain antigen detected by rat anti-C1300 antiserum. Nature. 1974 May 3;249(452):71–73. doi: 10.1038/249071a0. [DOI] [PubMed] [Google Scholar]
- Raiteri M., Bertollini A., La Bella R. Synaptosome antisera affect permeability of synaptosomal membranes in vitro. Nat New Biol. 1972 Aug 23;238(86):242–243. doi: 10.1038/newbio238242a0. [DOI] [PubMed] [Google Scholar]
- Schachner M. NS-1 (nervous system antigen-1), a glial-cell-specific antigenic component of the surface membrane. Proc Natl Acad Sci U S A. 1974 May;71(5):1795–1799. doi: 10.1073/pnas.71.5.1795. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schachner M., Sidman R. L. Distribution of H-2 alloantigen in adult and developing mouse brain. Brain Res. 1973 Sep 28;60(1):191–198. doi: 10.1016/0006-8993(73)90857-3. [DOI] [PubMed] [Google Scholar]
- Stallcup W. B., Cohn M. Correlation of surface antigens and cell type in cloned cell lines from the rat central nervous system. Exp Cell Res. 1976 Mar 15;98(2):285–297. doi: 10.1016/0014-4827(76)90440-7. [DOI] [PubMed] [Google Scholar]
- Toh B. H., Cauchi M. N. Brain-associated tumour antigens demonstrated by immunofluorescence. Nature. 1974 Aug 16;250(467):597–598. doi: 10.1038/250597a0. [DOI] [PubMed] [Google Scholar]
- WIGZELL H. QUANTITATIVE TITRATIONS OF MOUSE H-2 ANTIBODIES USING CR-51-LABELLED TARGET CELLS. Transplantation. 1965 May;3:423–431. doi: 10.1097/00007890-196505000-00011. [DOI] [PubMed] [Google Scholar]
- Warren S., Chute R. N. Pheochromocytoma. Cancer. 1972 Feb;29(2):327–331. doi: 10.1002/1097-0142(197202)29:2<327::aid-cncr2820290210>3.0.co;2-3. [DOI] [PubMed] [Google Scholar]
- Yavin E., Yavin Z. Attachment and culture of dissociated cells from rat embryo cerebral hemispheres on polylysine-coated surface. J Cell Biol. 1974 Aug;62(2):540–546. doi: 10.1083/jcb.62.2.540. [DOI] [PMC free article] [PubMed] [Google Scholar]