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. 2015 Feb 9;5:8339. doi: 10.1038/srep08339

Bioluminescence based biosensors for quantitative detection of enterococcal peptide–pheromone activity reveal inter-strain telesensing in vivo during polymicrobial systemic infection

Sabina Leanti La Rosa 1,*, Margrete Solheim 1, Dzung B Diep 1, Ingolf F Nes 1, Dag Anders Brede 1,a,*
PMCID: PMC4321189  PMID: 25661457

Abstract

Enterococcus faecalis is a significant threat in the nosocomial setting due to the emergence of isolates that are multi-antibiotic resistant, refractory to the available therapies and equipped with a variety of pathogenicity determinants. This bacterium uses quorum-sensing systems to regulate its physiological processes, including the expression of virulence traits, to adapt and proliferate within a host. Here, we describe the construction and application of two bioluminescence-based reporter systems for the direct detection of the quorum-sensing regulated expression of (i) the gelatinase biosynthesis-activating pheromone (GBAP) and (ii) the cytolysin small subunit (CylLS) in natural samples. The two E. faecalis reporters conditionally expressed bioluminescence in the presence of GBAP and CylLS both in the supernatants of liquid cultures and in an agar-overlay assay in as little as three hours, with a high level of sensitivity. Biosensors employed to investigate the interaction between the fsr and cyl systems revealed that fsr impeded CylLS activity by 75%. Furthermore, we identified a clinical E. faecalis isolate that acted as a biological cheater, producing cytolysin only upon sensing CylLS-producers in its environment. This isolate enhanced its virulence during polymicrobial systemic infection of Galleria mellonella.

Bacterial telesensing is guided by small extracellular molecules that are produced and secreted to actively query the environment1. Concentration or physiochemical changes in those ‘probes' are perceived as discharging environmental signals, allowing the bacteria to trigger an appropriate response in the host. Responses, such as enhancing their fitness for the colonisation of an environmental niche via subversion of the host's immune responses, releasing virulence factors as a defensive mechanism against competitive microorganisms or eukaryotic cells, differentiating into certain morphological forms in hostile environments and regulating genetic exchange, are coordinated through the telesensing systems1,2. The best characterised form of telesensing is quorum sensing (QS), in which bacteria sense and adapt to environmental conditions by coordinating and adjusting gene expression according to the local population density3.

Enterococcus faecalis is a gram-positive bacterium that commonly dwells in the gastrointestinal tract of healthy humans, animals and insects4,5. Although some strains have been safely used for decades as probiotics6, E. faecalis has rapidly emerged as a causative agent of hospital-acquired infections worldwide7. The spread of this opportunistic pathogen has been facilitated by its ability to tolerate and adapt to many types of environmental stresses and acquire high-level resistance to commonly used antibiotics8,9,10,11. The pathogenicity of E. faecalis has been linked to its production of factors involved in cell and tissue damage, adherence to cells and extracellular surface proteins, and evasion of the host immune system12. Three prominent peptide pheromone systems associated with highly virulent strains of E. faecalis are involved in environmentally regulated telesensing systems, including a conjugative system mediated by pheromone-responsive plasmids, the Fsr regulatory system, and cytolysin signalling13,14,15.

Cytolysin is a two-peptide lantibiotic haemolytic toxin of E. faecalis that requires the expression of two divergently organised multicistronic operons localised either on pheromone-responsive plasmids or on a pathogenicity island13,16. Two promoters, PLys for the structural genes and PReg for two regulators, control the expression of the cyl locus. Mature cytolysin consists of two peptides—CylLS and CylLL—and acts as a cytolytic toxin through forming a complex in eukaryotic and prokaryotic cellular membranes that leads to membrane rupture. Once synthesised, the two precursors are post-translationally modified by the product of the gene cylM and are secreted into the extracellular environment by the CylB transporter. Outside the cell, they eventually undergo a proteolytic activation through the action of CylA. In the absence of target cells, the larger subunit, CylLL, forms a stable inactive complex with the small subunit CylLS and inhibits its cytolytic activity. However, in the presence of a target, CylLL binds with a higher affinity to the cellular lipid membrane than to CylLS. As a consequence, the locally accumulating free mature CylLS will reach a certain threshold concentration that leads to the de-repressed binding of the regulatory proteins CylR1 and CylR2 on PLys and the autoinduction of the cytolysin operon. An additional gene, cylI, provides self-protection from the bactericidal effect of the toxin. Through this regulatory system, E. faecalis can therefore finely tune the expression of cyl genes according to the presence of target cells.

The Fsr system is a major virulence regulator in E. faecalis and comprises four genes that are responsive to the extracellular accumulation of the gelatinase biosynthesis-activating pheromone (GBAP)15,17. The PfsrB promoter initiates the transcription of an operon comprising three genes: fsrB, fsrD and fsrC. The fsrD gene encodes the precursor of GBAP, which is processed by the product of fsrB following its extracellular release. The local accumulation of the GBAP peptide is sensed by the histidine kinase FsrC, which is on the surface of E. faecalis, which then activates the response regulator FsrA by phosphorylation. FsrA acts as a transcription factor that up regulates expression through the PfsrB promoter as well as through a promoter that controls the coordinate expression of two virulence factors, the gelatinase GelE and the serine protease SprE.

In a recent study, our group constructed and assessed the use of two bioluminescence-based systems for the in vivo non-invasive monitoring of E. faecalis cytolysin- and gelatinase-promoter activity in the murine intestine and during the systemic infection of Galleria mellonella larvae and mice18. By determining the bioluminescence emission at different time points during the progression of an E. faecalis infection, we showed that both the gelatinase and cytolysin promoters were subjected to temporal regulation and that the expression of these traits was controlled in response to sensing diverse environmental conditions.

In this study, we describe the use of two bioluminescence-based reporter systems as biosensors for the direct detection and quantification of GBAP and CylLS in biological samples. The two biosensors are based on the PgelE and cylR2R1Pcyl promoters that drive the GBAP- or CylLS-induced expression of the luxABCDE operon specifically in the presence of true pheromone producers. Our results showed that the biosensors are suitable for the rapid, sensitive and real-time detection of positive isolates directly in natural samples and demonstrated for the first time that enterococcal telesensing both in vitro and in vivo during polymicrobial infection in G. mellonella larvae is possible.

Results and Discussion

Development of E. faecalis biosensors for detection of CylLS and GBAP pheromones

In a previous study, we utilised E. faecalis variants that expressed the luxABCDE cassette under the control of the cytolysin or gelatinase promoter to monitor the expression of the two factors during in vitro growth and during the infection of the mouse model. The results showed that the fsr and cyl QS- reporter systems were both modulated by environmental cues18.

In the current study, we constructed and employed lux-based biosensors for the detection and quantification of the cytolysin subunit CylLS and the gelatinase biosynthesis-activating pheromone (GBAP) of E. faecalis19,20.

The cytolysin biosensor was constructed by introducing the CylLS-responsive regulatory genes and the cyl promoter onto a lux-containing vector. The resulting plasmid, pSL101cylR2R1Pcyl, was introduced into E. faecalis JH2-218, a plasmid-free derivative of E. faecalis JH2, which lacks the cyl operon and is therefore unable to produce or sense the toxin21. For simplicity, in the following text, we will refer to the cytolysin biosensor as JH2-2 CBS.

To develop a functional GBAP biosensor system, we investigated the expression profile of both fsrB and gelE promoter-driven lux expression in E. faecalis MMH594. The growth of SL11 and SL13, which are, respectively PfsrB and PgelE reporter strains, at 37°C in GM17 medium was compared by monitoring the optical density at 620 nm. No significant difference in the growth rate or the final cell density was observed. This result indicated that the presence of the biosensor system did not hamper normal cell growth (Figure 1). Both promoters led to the emission of bioluminescence throughout growth, with similar expression patterns; in both strains, the signal was low during the early exponential phase and increased from the beginning of the mid-logarithmic phase, before waning during the stationary phase. Nevertheless, under the condition of equal amounts of pheromone, PfsrB showed a lower level of specific activity, with only a 10-fold increase, whereas PgelE-driven luxABCDE expression increased 230-fold during growth. These results are consistent with the outcomes of previous studies showing that the phosphorylated response regulator FsrA had a lower binding affinity for the fsrB promoter than for the gelE promoter15,22. Due to the higher level of expression driven by the PgelE promoter under the conditions tested, pREG696 luxPgelE was therefore selected for introduction into E. faecalis V583fsrB* for use as the GBAP reporter (aka V583fsrB* GBS).

Figure 1. Performance of the PgelE and PfsrB promoters during the growth of E. faecalis SL11 (MMH594::pREG696luxPgelE, in black) and SL13 (MMH594::pREG696luxPfsrB, in grey) in GM17 medium.

Figure 1

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The dotted lines indicate the promoter activity expressed as bioluminescence (photons/s) divided by the optical density (OD) at 620 nm, and growth is indicated by continuous lines and was measured as OD620. The values shown represent the averages of the results of three biological replicates ± standard deviation.

Biosensor proof of concept: Screening CylLS and GBAP production by genome-sequenced E. faecalis isolates

To test the ability of the JH2-2 CBS and V583fsrB* GBS to sense the presence of CylLS and GBAP producers in the environment, we employed nine genome-sequenced E. faecalis strains of clinical and commensal origin and developed a screening method that utilised GM17-agar plates. The strains had been previously tested for their cytolytic phenotype and GBAP-production ability (S. Leanti La Rosa, L. G. Snipen, B. E. Murray, R. Willems, M. S. Gilmore, D. B. Diep, I. F. Nes, and D. A. Brede, submitted). The panel included the cytolysin-positive strains DS5 and ×98, the GBAP-positive isolates E1Sol, V583 and V583ΔgelE, the GBAP- and cytolysin-producer MMH594, the GBAP- and cytolysin-negative strains T2 and CH188, and V583fsrB*, which harbours a mutation in the fsrB gene and is thus unable to synthesise the GBAP pheromone. Cells of the above-mentioned strains were cultured on two GM17-agar plates that were individually overlaid with the biosensors. Induction of visible light emission by the CylLS- and GBAP-producers but not by the non-producers occurred by 3 hours following the application of the appropriate biosensor (Figure 2). These bioluminescently tagged E. faecalis strains may therefore offer a simple, cost-effective and rapid method for determining the presence of cytolysin or GBAP producers in food, water, faecal and clinical samples. Furthermore, the systems were highly specific in sensing CylLS and GBAP and therefore would effectively prevent the false positive assumption of virulence traits based on only the detection of genes that might not necessarily lead to the corresponding phenotype.

Figure 2. Bioluminescence-based detection of CylLS (A) and GBAP (B) producers.

Figure 2

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From the right to the left, uppermost row: V583, DS5, and E1Sol; central row: V583fsrB*, CH188, and ×98; bottom row: MHH594, T2, and V583ΔgelE. The E. faecalis isolates were cultured on GM17 agar plates, and the biosensor was overlaid after an overnight incubation. Plates were kept at 37°C for 3 hours and imaged with a Xenogen IVIS Lumina II Imaging System (Calipers Corp., CA). A 10-fold higher light emission was induced by CylLS than by GBAP.

To assess the feasibility of the biosensor systems to directly detect cytolysin- and GBAP-positive strains in natural samples growing on enterococci-selective media, mixed cultures of clinical E. faecalis isolates were plated on bile esculin agar (BEA) and overlaid with JH2-2 CBS or V583fsrB* GBS biosensors. In both cases, the biosensor overlay allowed the identification of bioluminescence-inducing colonies in as little as three hours, confirming that the screening method was effective in detecting a specific producer within a sample and is suitable for real-time monitoring of pathogens without the need for pure cultures (Figure 3).

Figure 3. Bioluminescence imaging of CylLS (A) and GBAP (B) producers on BEA.

Figure 3

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The white arrows indicate cells that were unable to produce CylLS (A) or GBAP (B). After overnight incubation at 37°C, plates seeded with a mixed population of CylLs and GBAP producers were overlaid with the JH2-2 CBS (A) or V583fsrB* GBS (B), following additional incubation for 3 hours. Imaging was performed with a Xenogen IVIS Lumina II Imaging System (Calipers Corp., CA).

The JH2-2 CBS and V583fsrB* GBS detected high levels of CylLs- and GBAP-pheromone activities in cell-free culture supernatants

To corroborate the applicability of the CylLS and GBAP biosensors, we employed them for the direct detection and quantification of the two pheromones in liquid substrates. Whereas GBAP has been successfully isolated from the supernatants of E. faecalis cultures17, previous studies reported that cytolysin was clearly produced by E. faecalis growing on blood agar or when erythrocytes were added to broth cultures but a low level of activity was observed in liquid cultures23,24. It was therefore of interest to determine whether the CylLS and GBAP biosensors were able to specifically sense the local accumulation of mature pheromones in culture supernatants. To investigate GBAP detection, serial dilutions of supernatants from overnight and exponential-phase cultures of E. faecalis V583, V583ΔgelE and V583fsrB* were mixed with an equal amount of a 50-fold dilution of the GBAP biosensor in its exponential-phase. Bioluminescence was detected in the supernatants of the E. faecalis V583 and V583ΔgelE cultures but not in those of the V583fsrB* cultures, confirming the specificity of the construct, which reacted only to the gelatinase biosynthesis-inducing pheromone (Figure 4A). A highly significant correlation was found between the level of light emission in photons/s over a three-fold order of serial dilution of the samples of the supernatants of exponential- (R2 > 0.90) and stationary-phase cultures (R2 > 0.96), showing that this biosensor is an accurate and sensitive mean for quantifying the concentration of the GBAP pheromone. The measured GBAP activity was 320 GIU in exponential phase supernatants and 5120 GIU in overnight culture supernatant (data not shown). GBAP was detected at similar levels in the supernatants of both V583 and V583ΔgelE stationary- and exponential-growth phase cultures (P > 0.05, according to the Mann-Whitney test). This finding suggested that the gelatinase of these isolates did not inactivate or affect the biological activity of GBAP. The high levels of GBAP activity observed in the overnight cultures strongly suggested that the down-regulation of the fsr circuit during the stationary phase was not due to the inactivation of GBAP activity.

Figure 4.

Figure 4

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(A). Determination of GBAP-Inducing Units in an overnight (ON) culture and during the growth (at increasing OD620 units) of a culture of E. faecalis V583 wt (blue) or V583ΔgelE (red) V583fsrB* (violet) did not give rise to a detectable signal. (B). Determination of CylLS-Inducing Units during the growth of a culture and in an overnight (ON) culture of E. faecalis ×98 (green) and MMH594 (orange). The addition of the JH2-2 CBS to the T2 (light blue) supernatant did not give rise to a detectable signal at any dilution. GIU, GBAP inducing units; CIU, CylLS Inducing Units. * p-value > 0.05. ** p-value < 0.05.

Similarly, the JH2-2 CBS facilitated the specific detection of active CylLS in cell-free supernatants. JH2-2 CBS sensed up to 640 CIU in the supernatants of cells in the exponential phase of growth and 5120 CIU in overnight culture supernatant (data not shown). The exhibited levels of bioluminescence showed a linear dose-response dependency with the CylLS concentration (R2 > 0.97). This trend allowed the quantitative measurement of CylLS activity during bacterial growth. CylLS was found to accumulate in large amounts during the exponential growth phase of the CylLS-producers ×98 and MMH594 but was found to be totally absent in cultures of the non-producer T2 (Figure 4B). To the best of our knowledge, the presence of a high level of biologically active CylLS pheromone in the absence of target cells has not been reported hitherto. Surprisingly, the content of CylLS increased during the growth of in vitro monocultures, despite the fact that CylLS and CylLL are known to form non-haemolytic oligomers25. This result implied that cyl signalling was highly responsive even when the level of toxin activity was low.

Impairment of cytolysin production is mediated by the metalloprotease GelE

Interestingly, we observed a 2-fold difference in the levels of CylLS production in ×98 and MMH594 exponential-phase cultures, and a 4-fold higher level of CylLS in the supernatants of late exponential- and stationary-phase cultures of ×98 compared with those of MMH594 (P < 0.001, according to the results of the Mann-Whitney test) (Figure 4). In a recent study in which we investigated the pathogenicity of E. faecalis in the nematode Caenorhabditis elegans, statistical analyses showed that the concomitant expression of both gelatinase and cytolysin through the E. faecalis genome significantly increased its virulence (S. Leanti La Rosa, L. G. Snipen, B. E. Murray, R. Willems, M. S. Gilmore, D. B. Diep, I. F. Nes, and D. A. Brede, submitted). Interestingly, the effect of the co-presence of these two virulence traits was much less than the sum of their main individual effects, described as saturation or antagonistic effects. It was previously reported that GelE contribute to the virulence of E. faecalis by triggering the proteolytic degradation of a broad range of host substrates26,27,28, and gelE expression was found to have a profound effect on the secretome of E. faecalis29,30. GelE activity was found to be required for a variety of processes, such as the regulation of the display of the surface adhesin Ace31 and the activation of the primary autolysin AtlA and its contribution to biofilm formation32. Additionally, the production of gelatinase was reported to impair other cellular activities, such as conjugation due to the degradation of sex pheromone-related peptides33. Taken together with the results of utilising the CylLS-biosensor detection system, we considered the possibility that gelatinase might also have an impact on cytolysin. To test this hypothesis, we employed the JH2-2 CBS biosensor to detect CylLS accumulation in the supernatants of exponential- and stationary-phase cultures of the gelatinase-overproducing E. faecalis strain ×98::pCG (Figure 5). Under both of the tested conditions, our data showed that introducing gelatinase into the Cyl producer ×98 leads to a 4- to 8-fold reduction of CylLS activity (p < 0.05, according to the results of Mann-Whitney test).

Figure 5. Determination of CylLS-Inducing Units (CIU) in overnight (ON) cultures and during the growth (at increasing OD620 units) of cultures of E. faecalis ×98 pMG36c (red; control) and ×98 pCG (blue; GelE-overexpressing strain).

Figure 5

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The plot displays the averages of the results of triplicate independent experiments. * p-value < 0.05.

We previously observed that cytolysin is expressed mostly during the late exponential phase and that its expression gradually subsides during the stationary phase18. This trend is consistent with a scenario in which GelE is able to degrade part of the CylLS that was produced during the active growth phase and that GelE continued to exert its proteolytic activity long after the production of CylLS subsided. The fact that Cyl production was reduced by 75% was consistent with our previous observations regarding the pathogenicity of E. faecalis in C. elegans (S. Leanti La Rosa, L. G. Snipen, B. E. Murray, R. Willems, M. S. Gilmore, D. B. Diep, I. F. Nes, D. A. Brede, submitted). It thus appears probable that this phenomenon was highly effective in vivo.

Interstrain CylLS-mediated communication demonstrated the occurrence of an in vivo cheating behaviour

Quorum sensing is a specific type of telesensing that allows cell-cell communication via small diffusible molecules that are released to explore the surrounding environment34. In its simplest form, quorum sensing enables the bacteria to control the production of molecules that are released into the extracellular environment and become available not only for sibling producer cells but also for any other cells present. However, it has been reported that individuals that do not respond to quorum-sensing signals act as cheaters, not incurring the metabolic cost of producing those released molecules while benefiting from those secreted by cooperators35. The cheater gains a fitness advantage over the quorum-sensing positive strains. The production of extracellular metabolites, such as virulence factors, may lead to the recognition of the bacteria and ultimately destruction by the host immune system36. Therefore, quorum sensing can be used to trigger concerted gene activation in the microbial community to effectively respond to the prevailing conditions, which could be activating processes for evading a host or escaping the host's defences.

Studies have shown that the cyl locus is subject to genetic instability both in vitro and in vivo37,38. A number of investigators have reported phenotypic instabilities with no obvious explanations at the genetic level, indicating that silent or non-functional cyl genes occur in the genomes of enterococcal isolates of different origins39,40,41. In a previous report, we identified an apparent incongruence between the cyl genotype and phenotype in the clinical isolate T242 (S. Leanti La Rosa, L. G. Snipen, B. E. Murray, R. Willems, M. S. Gilmore, D. B. Diep, I. F. Nes and D. A. Brede, submitted). Here, we performed a detailed comparative sequence analysis of the cyl locus of a subset of genome-sequenced E. faecalis strains, including the strain MMH594, and we detected the presence of an IS6770 element that was integrated into the 3′-end of the cylA gene in T2 (Fig. 6A). The insertion causes a premature stop and the production of a truncated, and presumably non-functional, CylA (Fig. 6B).

Figure 6.

Figure 6

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(A) Alignment of the cyl operons of E. faecalis MMH594 and T2. A BlastN comparison was performed using EasyFig version 2.1 software. The levels of similarity ranged from 100 to 87%, as shown in the grey gradient scale. The light blue arrows indicate the genes. (B) Comparison of CylA sequences of MMH594 and T2. The conserved residues are highlighted in blue. The alignment was conducted using the MAFFT alignment program.

We then tested the hypothesis that E. faecalis strains exhibiting a cytolysin-negative phenotype but harbouring the elements of the cyl locus could act as social cheaters that cease production of quorum-controlled cytolysin and benefit from or take advantage of the release of CylLS by cooperators. A variant of E. faecalis T2 tagged with pSL101cylR2R1Pcyl (named T2 CBS) was employed; the assessment of cytolysin production mediated by sensing the local accumulation of mature CylLS subunits in the environment was performed by growing E. faecalis T2 CBS in proximity to the cytolysin-positive strain MMH594 on blood-agar plates (Figure 7). A diffuse and bright haemolytic zone was observed in the area in which the two strains were in proximity to each other (Fig. 7A) indicating that E. faecalis T2 CBS was able to detect and use the CylLS released by the MMH594 cells to trigger its own cytolysin production. Imaging analysis showed the ‘flare'-like induction of bioluminescence throughout the streak of T2 CBS (Figure 7C). Haemolysis or the ‘flare' effect was not detected when JH2-2, a strain lacking all of the genes necessary for cytolysin production, was streaked near T2 CBS cells (data not shown). In this case, bioluminescence arose only from the JH2-2 CBS cells in close proximity to MMH594 cells. No increase in the level of haemolysis or light emissions was observed when MMH594 or T2 CBS cells were cultivated in proximity to E. faecalis CH188, which lacks a complete cyl operon43 (Figures 7B and 7D). In addition, no haemolysis was observed when T2 CBS was cultivated alone or in proximity to its parental strain on blood-agar plates (data not shown). To test whether the cytolysin-positive phenotype was stably imposed on the T2 cells, cells that had been induced once were re-streaked on blood agar. However, these cells consistently reverted to a cytolysin-negative phenotype (data not shown). Based on these results, we hypothesised that E. faecalis T2 acted as a social cheater, adopting the strategy of reducing its metabolic burden by avoiding the production and release of the toxin and benefiting from a growth advantage over cytolysin-producing cells. However, this strain retained its immunity to the bactericidal effect and its ability to exploit the release of CylLS into the environment by a primary producer to efficiently spread during a polymicrobial infection.

Figure 7. E. faecalis interstrain communication.

Figure 7

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Cultivating E. faecalis T2 CBS in proximity to MMH594 induced cytolysin production by T2, visible as a clear zone, (A) and bioluminescence emissions that propagated to the top of the streak (C), whereas no induction was detected when T2 CBS was cultivated near CH188 (D). The control isolate CH188 maintained its cytolysin-negative phenotype when cultivated in proximity to MMH594 (B). Experiments were performed on BHI plates supplemented with 1% (v/v) defibrinated horse blood, 1% (w/v) glucose and 0.03% (w/v) L-arginine.

In vivo cytolysin telesensing during infections of G. mellonella larvae exacerbated the virulence

A previous study indicated that appropriate regulation of both the gelatinase and the cytolysin promoter occurred during the mono-infection of G. mellonella larvae with lux-tagged E. faecalis MMH594 variants and that these traits were conditionally induced in response to the insect haemocoel environment18. To investigate whether the interstrain communication observed in T2 CBS (as described above) could affect pathogenicity in vivo, we performed a polymicrobial infection of G. mellonella larvae with E. faecalis T2 CBS in combination with the cytolysin-producer ×98. Injecting 2 × 106 T2 CBS cells resulted in an LT50 of approximately 13 hours (Figure 8A) and low levels of bioluminescence (Figure 8B). Injecting an equal amount of inoculum of T2 CBS in combination with ×98 at a dose of approximately 2 × 105 cells into the haemocoel of the insects resulted in an increased level of virulence, with an LT50 of 11 hours (P = 0.015, chi-square test with 1 degree of freedom, T2 CBS + ×98 versus T2 CBS). Moreover, the 100-fold increase in bioluminescence observed at 6 hours post infection was indicative of interstrain CylLS communication. To explore the possibility that the increased pathogenicity could be due to the presence of ×98 or was a result of induced cytolysin production by T2, a similar experiment was performed using the JH2-2 CBS instead of the T2 CBS, because the JH2-2 strains lacks the cyl operon. In this experiment, the JH2-2 CBS demonstrated CylLS interstrain communication, with a level of bioluminescence induction similar to that observed when T2 was used as the biosensor (Figure 8B), but there was no increase in the level of virulence (Figure 8A). Collectively, these results suggested that in vivo telesensing was a credible scenario during polymicrobial enterococcal infections that potentially could contribute to increasing the severity of such infections. These findings might have broad implications because a number of apparently cyl-negative phenotypic isolates contain intact cyl loci or a deletion in the cylA proteinase gene40,41. It is conceivable that such strains would both be immune to the cytolysin toxin and demonstrate cytolytic activity when sharing a habitat with a CylA-expressing strain. Alternatively, clinical isolates such as T2, which bears defective cyl loci, might have evolved during infection via a mechanism similar to that by which V583 descended from V58644.

Figure 8. G. mellonella larvae were injected by E. faecalis ×98 (purple), T2 CBS (orange), JH2-2 CBS (green), and a mixed suspension of either ×98 + T2 CBS (blue) or ×98 + JH2-2 CBS (red).

Figure 8

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(A). Survival curves of G. mellonella larvae following infection with E. faecalis. Co-infection by T2 CBS and ×98 caused increased killing to that resulting by T2 CBS mono-infection, (P = 0.015, chi-square test with 1 degree of freedom, T2 CBS + ×98 versus T2 CBS). The plots display the averages of results of triplicate independent experiments. For each assay, 10 insects were used. (B). Real-time monitoring of CylLS expression during the progression of an E. faecalis infection in intact insects. For tracking bacterial infection in G. mellonella, five individual insects were injected and placed in duplicate in a 4.0 cm Petri dish. Plates were kept at 37°C in the chamber of the Xenogen IVIS Lumina II imaging system (Caliper Life Sciences, CA). Bioluminescence was recorded at 30 minutes intervals for 20 hours.

Using bioluminescence imaging, we were able to follow the real-time dynamics of the CylLS levels in intact insects during a mortality assay (Figure 8B). No light was detectable at any time point in the larvae infected with ×98, which confirmed the absence of any background bioluminescence. A basal level of light emission was observed in the insects that had been injected with T2 CBS; however, the level of bioluminescence reached a peak at 5 h post infection and diminished progressively. Despite this basal signal, a 716-fold increase in the level of bioluminescence was observed in the larvae that had been co-infected with both isolates. The signal caused by infection with the mixed culture of T2 CBS and ×98 was 60-fold higher than the basal signal; the signal progressively increased and peaked at 8 h post-infection, concomitant with when the insects began to die, and persisted at this level throughout the death of the wax moths. Similarly, co-infection with JH2-2 CBS and ×98 gave rise to a 69–fold higher bioluminescence emission than the basal signal from larvae injected with JH2-2 CBS (Figure 8B).

Taken together, the evident change in the levels of virulence and Pcyl-driven luxABCDE expression that was observed during G. mellonella co-infection supported the hypothesis that a small population of CylLS producers can induce cytolysin synthesis by responsive cheaters during a polymicrobial infection.

Conclusions

In this report, we describe the construction of two biosensors for the simple and rapid detection of CylLS and the gelatinase biosynthesis-activating pheromone in E. faecalis. We showed that these reporters can be used for the real-time identification of pheromone producers both on agar plates and from culture supernatants and illustrated the applicability of the constructs to natural samples, alleviating the need for pure cultures. The biosensors were used to monitor the production of the pheromones GBAP and CylLS during growth and they detected quantitative between-strain differences in CylLS activity.

Pursuing this observation, we showed that gelatinase had an antagonistic activity toward cytolysin production, probably through its proteolytic degradation of the cytolysin-toxin subunits. To the best of our knowledge, this is the first example of an antagonistic interaction between two virulence traits that has been demonstrated in E. faecalis.

We also used the biosensors to elucidate a novel type of social cheating mechanism, which enabled conditional toxin production in a certain strain of E. faecalis. The production of cytolysin by the cheater strain was dependent upon its recognition of the accumulation of CylLS produced by another cyl-expressing strain in its surrounding environment. The relevance of this mechanism was corroborated during the polymicrobial infection of the G. mellonella model system. Therefore, these reporter systems represent a powerful tool for studying E. faecalis pathogenicity, which will allow the population dynamics of cytolysin and GBAP producers to be monitored and will expand the current knowledge of the expression and functional activity of E. faecalis genes in microbe-host interactions.

Methods

Bacterial strains, plasmids and growth conditions

The bacterial strains used in this study are listed in Table 1. Unless otherwise indicated, the E. faecalis strains were routinely cultivated at 37°C, without agitation, in M17 broth (Oxoid LTD, UK) supplemented with 0.4% w/v glucose (GM17). The E. coli strains were grown at 37°C, with shaking, in Luria-Bertani (LB, Oxoid LTD, UK) broth. The following antibiotic concentrations were used for the enterococci: spectinomycin, 500 μg/mL and chloramphenicol, 20 μg/mL. The following antibiotic concentrations were used for the E. coli: spectinomycin, 200 μg/mL and chloramphenicol, 10 μg/mL.

Table 1. Bacterial strains and plasmids used in this study.

Name Descriptiona Reference
E. faecalis    
DS5 Cytolysin-positive clinical isolate 48
E1Sol Gelatinase-positive commensal strain 49
CH188 Gelatinase- and cytolysin-negative clinical isolate 43
X98 Cytolysin-positive isolate 50
JH2-2 Gelatinase- and cytolysin-negative laboratory strain 21
T2 Gelatinase- and cytolysin-negative clinical isolate 42
MMH594 Gelatinase- and cytolysin-positive clinical isolate 51
V583 Gelatinase-positive clinical isolate 44
V583fsrB* E. faecalis V583 with an amber point mutation in the fsrB codon that causes the loss of GBAP production; gelatinase-negative phenotype Leanti La Rosa et al, submitted
V583ΔgelE E. faecalis V583 gelE mutant; produces GBAP but has a gelatinase-negative phenotype 45
SL11 E. faecalis MMH594::pREG696 luxPgelE This study
SL13 E. faecalis MMH594::pREG696 luxPfsrB This study
JH2-2 CBS E. faecalis JH2-2::pSL101cylR2R1Pcyl This study
T2 CBS E. faecalis T2::pSL101cylR2R1Pcyl This study
V583fsrB* GBS E. faecalis V583 fsrB*::pREG696 luxPgelE This study
X98 pMG36c E. faecalis ×98:: pMG36c This study
X98 pCG E. faecalis ×98:: pCG This study
Plasmids    
pSL101cylR2R1Pcyl Spcr, contains the axe-txe cassette and the luxABCDE operon under the control of the Pcyl promoter and the regulatory genes cylR2 and cylR1 18
pREG696 luxPgelE Spcr, contains the gelE promoter fused to the luxABCDE operon 18
pREG696 luxPfsrB Spcr, contains the fsrB promoter fused to the luxABCDE operon 45
pMG36c Camr, 52
pCG Camr 53
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aSpcr, spectinomycin resistance; Camr, chloramphenicol resistance.

The E. faecalis CylLS-biosensor strains (CBS) were constructed by introducing the pSL101cylR2R1Pcyl vector into E. faecalis strains JH2-2 and T218. To develop a GBAP biosensor (GBS), the following two vectors were employed: pREG696 luxPfsrB45, which contained a 480-bp segment of the upstream region and the ATG codon of fsrB fused to the luxABCDE cassette of pPL2 lux and the axe-txe stability module, and pREG696 luxPgelE18.

The vectors were propagated in E. coli GeneHogs, and the plasmid DNA was isolated using the E.Z.N.A. Plasmid Mini Kit I (Omega Bio-tek, USA) prior to transferring it into the E. faecalis strains via electro-transformation46. The transformants were selected using GM17 plates containing spectinomycin.

Assessment of PfsrB and PgelE promoter activities during in vitro growth

The growth and the luminescence expression driven by the PfsrB and PgelE promoter of the SL11 and SL13 E. faecalis strains, respectively, were evaluated as described previously47. Briefly, E. faecalis overnight (ON) cultures were diluted 100-fold using GM17 medium, grown at 37°C until they reached an optical density at 620 nm of 0.2 and then diluted 100-fold again using fresh GM17 broth. A 300-μl aliquot of the culture was added to the wells of a 96-well plate (Nunc, Thermo Fisher Scientific, Denmark) and the plate was incubated at 37°C under static conditions in a Spectrostar Nano microplate reader (BMG Labtech). The absorbance at 620 nm was measured at 15-min intervals for 7 h. For the bioluminescence measurements, 300 μl of the same culture was added to the wells of a black 96-well plate (Nunc, Thermo Fisher Scientific, Denmark), which was incubated at 37°C under static conditions in the chamber of a Xenogen IVIS Lumina II Imaging System (Calipers Corp., CA). Luminescence was measured every 15 min for 7 h, with a binning factor of 16, F-stop of 1 and an exposure time of 1 minute.

To determine the specific promoter activities, the photons/second emissions of the different lux-tagged E. faecalis cultures were normalised according to their growth, as expressed as the optical density (OD) at 620 nm.

Validation of the specificity of the CylLS- and GBAP-producing colony-screening assays

Single colonies of genome-sequenced E. faecalis isolates that are known to produce CylLS and/or GBAP were cultured overnight in GM17 broth at 37°C. A 2.5-μl aliquot of each culture was spotted onto two GM17 agar plates, which were incubated for 16 h at 37°C. The plates were subsequently overlaid with 10 mL of GM17 soft agar (0.8% w/v agar) that had been tempered to 50°C and were seeded with 200 μl of an overnight biosensor culture. After incubation at 37°C for 3 h, the plates were visualised using a Xenogen IVIS Lumina II Imaging System (Calipers Corp., CA), using a binning factor of 16, F-stop of 1 and an exposure time of 1 minute.

Identification of CylLS and GBAP producers on enterococci-selective plates

A mixed culture containing two known CylLS and GBAP producers was plated on two bile-aesculin agar (BEA, Oxoid LTD., UK) plates, which were incubated at 37°C overnight. The dilution factor of the culture was adjusted so that the plating resulted in the production of 20–30 colonies per plate. After performing the biosensor overlay as described above, the plates were maintained at 37°C for 3 hours and were visualised using a Xenogen IVIS Lumina II Imaging System (Calipers Corp., CA), using a binning factor of 16, F-stop of 1 and an exposure time of 3 minutes.

Detection of CylLS and GBAP in E. faecalis supernatants

CylLS and GBAP detection in the supernatants of cultures of genome-sequenced E. faecalis producers was performed using the biosensors JH2-2 CBS and V583fsrB* GBS, respectively. Single colonies of E. faecalis ×98, MMH594, T2, V583, V583ΔgelE and V583fsrB* were inoculated into 5 mL of GM17 broth, and the cultures were incubated overnight at 37°C. The cultures were then diluted 1:100 using fresh GM17 broth and grown at 37°C; aliquots of the cultures were taken when the OD620 values were 0.1, 0.25, 0.5 and 1.0. After centrifugation at 10,000 × g for 10 minutes to remove the cells, the culture supernatants were collected and stored at 4°C until use. The supernatants were subjected to serial 2-fold dilutions in a volume of 100 μl/well in a 96-well plate (Nunc, Thermo Fisher Scientific, Denmark), and 100 μl of a 50× diluted exponential phase culture or an ON culture of the appropriate biosensor was added to each well. After incubation at 37°C for 3 h, the levels of bioluminescence emission were measured using a Xenogen IVIS Lumina II Imaging System, using a binning factor of 16, F-stop of 1 and an exposure time of 1 minute.

One CylLS inducing unit (CIU) was defined as the reciprocal of the highest level of dilution of a sample that provided a 2-fold increase in the level of bioluminescence of a 0.2-mL aliquot of a JH2-2 CBS culture compared with the level of basal expression. Similarly, 1 GBAP-inducing unit (GIU) was defined as the reciprocal of the highest level of dilution of a sample that provided a 2-fold increase in the level of bioluminescence of a 0.2-mL aliquot of a 0.2 mL culture of V583fsrB* GBS compared with the level of basal expression.

In vitro detection of cytolysin-mediated interstrain communication

The cytolysin-positive E. faecalis strain MMH594 was inoculated onto a blood-agar plate (Brain-heart infusion agar supplemented with 5% (v/v) defibrinated horse blood, 1% (w/v) glucose and 0.03% (w/v) L-arginine (Sigma-Aldrich)) by creating a curved streak that reached the centre of the plate. Samples of E. faecalis CH188 or T2 CBS, which exhibited a negative haemolytic phenotype, were similarly streaked on the right side of the plate, 2-mm from and not touching the MMH594 cells. As a control, a plate was similarly seeded with CH188 and T2 CBS cells. The plates were incubated at 37°C for 16 h under anaerobic conditions. Imaging of the plates was performed as described above.

In vivo CylLS telesensing during polymicrobial systemic infection of G. mellonella using E. faecalis

G. mellonella larvae were infected with E. faecalis as previously described, with some minor modifications47. Briefly, exponential-phase cultures of E. faecalis ×98 and T2 CBS grown in GM17 broth were washed three times using a sterile 0.9% saline solution and were brought to concentrations of 2 × 107 ± 0.8 × 107 CFU/mL and 2 × 108 ± 1.8 × 108 CFU/mL, respectively. For the purpose of co-infection, ×98 and T2 CBS cells were mixed in a ratio of 1:10 v/v. Larvae (weighing approximately 3 mg and approximately 3 cm in length) were injected through the left hindmost proleg with 10 μl of E. faecalis solution using a Hamilton 710SNR 100-μL syringe (Hamilton Company) fitted with a 30 G needle (BD Microlance 3). For each assay, 10 insects were used in triplicate and the experiment was independently repeated at least two times. The larval survival rate was determined at 20 hours after infection. For real-time visualisation of E. faecalis infection in G. mellonella, five individual insects were injected as described above and were placed in duplicate in a 4.0-cm Petri dish. The plates were incubated at 37°C in the chamber of a Xenogen IVIS Lumina II imaging system (Caliper Life Sciences, CA) and the bioluminescence emissions were recorded at 30-minute intervals for 20 hours. The LT50 values (time at which the lethality of 50% of the insects was reached during a 20-hour period) were employed to compare the level of infectivity of the inoculum.

Author Contributions

D.A.B., S.L.L.R. and M.S. designed and performed the experiments and analysed the data; D.A.B., D.B.D. and I.F.N. contributed to reagents/materials/analytical tools. S.L.L.R. and D.A.B. wrote the paper. All of the authors reviewed the final manuscript.

Acknowledgments

We thank Linda Godager for her technical assistance. This study was supported by grant number 191452 from the Research Council of Norway.

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