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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Nov;74(11):5162–5165. doi: 10.1073/pnas.74.11.5162

Regional dissociation of beta-endorphin and enkephalin contents in rat brain and pituitary.

J Rossier, T M Vargo, S Minick, N Ling, F E Bloom, R Guillemin
PMCID: PMC432121  PMID: 270754

Abstract

beta-Endorphin and enkephalin in extracts of whole brain, various brain regions, adenohypophysis, and combined pars intermedia and neurohypophysis of the rat were measured by radioimmunoassay. In brain extracts, the immunoreactive substances were further separated according to molecular size by gel filtration. beta-Endorphin was found in the diencephalon but not in the hippocampus, cerebral cortex, cerebellum, and striatum. Enkephalin was found predominantly in the striatum and diencephalon. Attention is called to possible artifactual interference by myelin basic protein in the immunoassays for beta-endorphin in some regions of the brain. In the pituitary, enkephalin was mainly restricted to the pars intermedia-neurohypophysis. Neither adrenalectomy nor hypophysectomy significantly altered levels of beta-endorphin in brain extracts. Adrenalectomy increased the levels of beta-endorphin in adenohypophysis and pars intermedia-neurohypophysis; after adrenalectomy, enkephalin was also increased in the adenohypophysis but less so in the pars intermedia-neurohypophysis. These results show that brain endorphin levels are independent of pituitary endorphin levels; they suggest that beta-endorphin-containing neurons and those containing enkephalin constitute two separate groups of brain cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birdsall N. J., Hulme E. C. C fragment of lipotropin has a high affinity for brain opiate receptors. Nature. 1976 Apr 29;260(5554):793–795. doi: 10.1038/260793a0. [DOI] [PubMed] [Google Scholar]
  2. Bloom F., Battenberg E., Rossier J., Ling N., Leppaluoto J., Vargo T. M., Guillemin R. Endorphins are located in the intermediate and anterior lobes of the pituitary gland, not in the neurohypophysis. Life Sci. 1977 Jan 1;20(1):43–47. doi: 10.1016/0024-3205(77)90126-6. [DOI] [PubMed] [Google Scholar]
  3. Cox B. M., Goldstein A., Hi C. H. Opioid activity of a peptide, beta-lipotropin-(61-91), derived from beta-lipotropin. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1821–1823. doi: 10.1073/pnas.73.6.1821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dragon N., Seidah N. G., Lis M., Routhier R., Chrétien M. Primary structure and morphine-like activity of human beta-endorphin. Can J Biochem. 1977 Jun;55(6):666–670. doi: 10.1139/o77-096. [DOI] [PubMed] [Google Scholar]
  5. Elde R., Hökfelt T., Johansson O., Terenius L. Immunohistochemical studies using antibodies to leucine-enkephalin: initial observations on the nervous system of the rat. Neuroscience. 1976 Aug;1(4):349–351. doi: 10.1016/0306-4522(76)90063-4. [DOI] [PubMed] [Google Scholar]
  6. Glowinski J., Iversen L. L. Regional studies of catecholamines in the rat brain. I. The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain. J Neurochem. 1966 Aug;13(8):655–669. doi: 10.1111/j.1471-4159.1966.tb09873.x. [DOI] [PubMed] [Google Scholar]
  7. Graf L., Szekely J. I., Ronai A. Z., Dunai-Kovacs Z., Bajusz S. Comparative study on analgesic effect of Met5-enkephalin and related lipotropin fragments. Nature. 1976 Sep 16;263(5574):240–242. doi: 10.1038/263240a0. [DOI] [PubMed] [Google Scholar]
  8. Guillemin R., Ling N., Burgus R. Endorphines, peptides, d'origine hypothalamique et neurohypophysaire à activité morphinomimétique. Isolement et structure moléculaire de l'alpha-endorphine. C R Acad Sci Hebd Seances Acad Sci D. 1976 Feb 23;282(8):783–785. [PubMed] [Google Scholar]
  9. Guillemin R., Ling N., Vargo T. Radioimmunoassays for alpha-endorphin and beta-endorphin. Biochem Biophys Res Commun. 1977 Jul 11;77(1):361–366. doi: 10.1016/s0006-291x(77)80205-2. [DOI] [PubMed] [Google Scholar]
  10. Hughes J., Smith T. W., Kosterlitz H. W., Fothergill L. A., Morgan B. A., Morris H. R. Identification of two related pentapeptides from the brain with potent opiate agonist activity. Nature. 1975 Dec 18;258(5536):577–580. doi: 10.1038/258577a0. [DOI] [PubMed] [Google Scholar]
  11. Krieger D. T., Liotta A., Suda T., Palkovits M., Brownstein M. J. Presence of immunoassayable beta-lipotropin in bovine brain and spinal cord: lack of concordance with ACTH concentrations. Biochem Biophys Res Commun. 1977 Jun 6;76(3):930–936. doi: 10.1016/0006-291x(77)91591-1. [DOI] [PubMed] [Google Scholar]
  12. Lazarus L. H., Ling N., Guillemin R. beta-Lipotropin as a prohormone for the morphinomimetic peptides endorphins and enkephalins. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2156–2159. doi: 10.1073/pnas.73.6.2156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Li C. H., Yamashiro D., Tseng L. F., Loh H. H. Synthesis and analgesic activity of human beta-endorphin. J Med Chem. 1977 Mar;20(3):325–328. doi: 10.1021/jm00213a001. [DOI] [PubMed] [Google Scholar]
  14. Ling N., Guillemin R. Morphinomimetic activity of synthetic fragments of beta-lipotropin and analogs. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3308–3310. doi: 10.1073/pnas.73.9.3308. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mains R. E., Eipper B. A., Ling N. Common precursor to corticotropins and endorphins. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3014–3018. doi: 10.1073/pnas.74.7.3014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nicoll R. A., Siggins G. R., Ling N., Bloom F. E., Guillemin R. Neuronal actions of endorphins and enkephalins among brain regions: a comparative microiontophoretic study. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2584–2588. doi: 10.1073/pnas.74.6.2584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Simantov R., Kuhar M. J., Uhl G. R., Snyder S. H. Opioid peptide enkephalin: immunohistochemical mapping in rat central nervous system. Proc Natl Acad Sci U S A. 1977 May;74(5):2167–2171. doi: 10.1073/pnas.74.5.2167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Simantov R., Snyder S. H. Morphine-like peptides in mammalian brain: isolation, structure elucidation, and interactions with the opiate receptor. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2515–2519. doi: 10.1073/pnas.73.7.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yang H. Y., Hong J. S., Costa E. Regional distribution of LEU and MET enkephalin in rat brain. Neuropharmacology. 1977 Apr;16(4):303–307. doi: 10.1016/0028-3908(77)90112-5. [DOI] [PubMed] [Google Scholar]

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