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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Jun;74(6):2288–2292. doi: 10.1073/pnas.74.6.2288

Translation of tobacco necrosis virus and its satellite in a cell-free wheat germ system.

M S Salvato, H Fraenkel-Conrat
PMCID: PMC432155  PMID: 267924

Abstract

Tobacco necrosis virus (TNV) and its satellite virus (STNV) each contain a single-stranded RNA genome, of about 1.4 X 10(6) and 0.4 X 10(6) daltons, respectively, which is very active in stimulating amino acid incorporation in a wheat germ cell-free system. With STNV RNA the predominant incorporation product of 22,000 daltons coelectrophoreses with viral coat protein and crossreacts with antibody to viral coat protein. A similar result is obtained with TNV RNA, the only major translation product being a 30,000-dalton protein which corresponds to the coat protein by gel sizing, serological tests, and tryptic peptide analysis. Other products appearing in smaller amounts are about 63,000, 43,000, and 26,000 daltons and smaller. The possible nature of these products is discussed, as well as the unusual feature of a large, presumably multigenic, viral RNA yielding the coat protein as the predominant translation product in a eukaryotic system. Much less STNV RNA than TNV RNA produces maximal translation. Cotranslation of both RNAs in vitro indicates that STNV RNA has a translational advantage over TNV RNA. The fact that these RNAs lack 5'-terminal capping and 3'-terminal poly(A) is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fraenkel-Conrat H. RNA polymerase from tobacco necrosis virus infected and uninfected tobacco. Purification of the membrane-associated enzyme. Virology. 1976 Jul 1;72(1):23–32. doi: 10.1016/0042-6822(76)90308-1. [DOI] [PubMed] [Google Scholar]
  2. Higgins T. J., Goodwin P. B., Whitfeld P. R. Occurrence of short particles in beans infected with the cowpea strain of TMV. II. Evidence that short particles contain the cistron for coat-protein. Virology. 1976 Jun;71(2):486–497. doi: 10.1016/0042-6822(76)90376-7. [DOI] [PubMed] [Google Scholar]
  3. Horst J., Fraenkel-Conrat H., Mandeles S. Terminal heterogeneity at both ends of the satellite tobacco necrosis virus ribonucleic acid. Biochemistry. 1971 Dec 7;10(25):4748–4752. doi: 10.1021/bi00801a022. [DOI] [PubMed] [Google Scholar]
  4. Hunter T. R., Hunt T., Knowland J., Zimmern D. Messenger RNA for the coat protein of tobacco mosaic virus. Nature. 1976 Apr 29;260(5554):759–764. doi: 10.1038/260759a0. [DOI] [PubMed] [Google Scholar]
  5. Jones I. M., Reichmann M. E. The proteins synthesized in tobacco leaves infected with tobacco necrosis virus and satellite tobacco necrosis virus. Virology. 1973 Mar;52(1):49–56. doi: 10.1016/0042-6822(73)90397-8. [DOI] [PubMed] [Google Scholar]
  6. Klein C., Fritsch C., Briand J. P., Richards K. E., Jonard G., Hirth L. Physical and functional heterogeneity in TYMV RNA: evidence for the existence of an independent messenger coding for coat protein. Nucleic Acids Res. 1976 Nov;3(11):3043–3061. doi: 10.1093/nar/3.11.3043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klein W. H., Nolan C., Lazar J. M., Clark J. M., Jr Translation of satellite tobacco necrosis virus ribonucleic acid. I. Characterization of in vitro procaryotic and eucaryotic translation products. Biochemistry. 1972 May 23;11(11):2009–2014. doi: 10.1021/bi00761a003. [DOI] [PubMed] [Google Scholar]
  8. Lesnaw J. A., Reichmann M. E. Identity of the 5'-terminal RNA nucleotide sequence of the satellite tobacco necrosis virus and its helper virus: possible role of the 5'-terminus in the recognition by virus-specific RNA replicase. Proc Natl Acad Sci U S A. 1970 May;66(1):140–145. doi: 10.1073/pnas.66.1.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leung D. W., Gilbert C. W., Smith R. E., Sasavage N. L., Clark J. M., Jr Translation of satellite tobacco necrosis virus ribonucleic acid by an in vitro system from wheat germ. Biochemistry. 1976 Nov 2;15(22):4943–4950. doi: 10.1021/bi00667a030. [DOI] [PubMed] [Google Scholar]
  10. Marcu K., Dudock B. Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ. Nucleic Acids Res. 1974 Nov;1(11):1385–1397. doi: 10.1093/nar/1.11.1385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mayo M. A., Fritsch C., Hirth L. Translation of tobacco rattle virus RNA in vitro using wheat germ extracts. Virology. 1976 Feb;69(2):408–415. doi: 10.1016/0042-6822(76)90472-4. [DOI] [PubMed] [Google Scholar]
  12. Pawson T., Martin G. S., Smith A. E. Cell-free translation of virion RNA from nondefective and transformation-defective Rous sarcoma viruses. J Virol. 1976 Sep;19(3):950–967. doi: 10.1128/jvi.19.3.950-967.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pleij C. W., Neeleman A., van Vloten-Doting L., Bosch L. Translation of turnip yellow mosaic virus RNA in vitro: a closed and an open coat protein cistron. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4437–4441. doi: 10.1073/pnas.73.12.4437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rice R., Fraenkel-Conrat H. Fidelity of translation of satellite tobacco necrosis virus ribonucleic acid in a cell-free Escherichia coli system. Biochemistry. 1973 Jan 16;12(2):181–187. doi: 10.1021/bi00726a001. [DOI] [PubMed] [Google Scholar]
  15. Roman R., Brooker J. D., Seal S. N., Marcus A. Inhibition of the transition of a 40 S ribosome-Met-tRNA-i-Met complex to an 80 S ribosome-Met-tRNA-i-Met- complex by 7-Methylguanosine-5'-phosphate. Nature. 1976 Mar 25;260(5549):359–360. doi: 10.1038/260359a0. [DOI] [PubMed] [Google Scholar]
  16. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]

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