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. 1977 Jun;74(6):2330–2333. doi: 10.1073/pnas.74.6.2330

Nerve growth factor in mouse serum and saliva: role of the submandibular gland.

R A Murphy, J D Saide, M H Blanchard, M Young
PMCID: PMC432164  PMID: 267927

Abstract

The concept that the salivary gland of the mouse is an endocrine organ for nerve growth factor (NGF) has been reexamined. Serum concentrations of the protein have been measured by radioimmunoassay in male and female mice and in mice from which the submandibular glands were removed. In spite of the fact that the submandibular glands of male mice contained more NGF than did those of female mice, no sex differences in circulating concentrations of the factor were detected. Furthermore, serum concentrations of NGF did not change after submandibular gland removal or after administration of several autonomic agonists. These results indicate that the submandibular glands are not endocrine organs with respect to NGF. On the other hand, extremely high concentrations of the factor are normally secreted in mouse saliva at levels that reflect the sex differences in the amount of NGF present in the glands. This finding suggests that the salivary gland is an exocrine organ for NGF and that the protein may play a biological role in saliva.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Angeletti P., Calissano P., Chen J. S., Levi-Montalcini R. Multiple molecular forms of the nerve growth factor. Biochim Biophys Acta. 1967 Sep 19;147(1):180–182. doi: 10.1016/0005-2795(67)90104-3. [DOI] [PubMed] [Google Scholar]
  2. Bocchini V., Angeletti P. U. The nerve growth factor: purification as a 30,000-molecular-weight protein. Proc Natl Acad Sci U S A. 1969 Oct;64(2):787–794. doi: 10.1073/pnas.64.2.787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen S., Savage C. R., Jr Recent studies on the chemistry and biology of epidermal growth factor. Recent Prog Horm Res. 1974;30(0):551–574. doi: 10.1016/b978-0-12-571130-2.50018-3. [DOI] [PubMed] [Google Scholar]
  4. Gregory H. Isolation and structure of urogastrone and its relationship to epidermal growth factor. Nature. 1975 Sep 25;257(5524):325–327. doi: 10.1038/257325a0. [DOI] [PubMed] [Google Scholar]
  5. Hendry I. A. Developmental changes in tissue and plasma concentrations of the biologically active species of nerve growth factor in the mouse, by using a two-site radioimmunoassay. Biochem J. 1972 Aug;128(5):1265–1272. doi: 10.1042/bj1281265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hogue-Angeletti R. A., Frazier W. A., Jacobs J. W., Niall H. D., Bradshaw R. A. Purification, characterization, and partial amino acid sequence of nerve growth factor from cobra venom. Biochemistry. 1976 Jan 13;15(1):26–34. doi: 10.1021/bi00646a005. [DOI] [PubMed] [Google Scholar]
  7. Ishii D. N., Shooter E. M. Regulation of nerve growth factor synthesis in mouse submaxillary glands by testosterone. J Neurochem. 1975 Dec;25(6):843–851. doi: 10.1111/j.1471-4159.1975.tb04416.x. [DOI] [PubMed] [Google Scholar]
  8. Levi-Montalcini R., Angeletti P. U. Nerve growth factor. Physiol Rev. 1968 Jul;48(3):534–569. doi: 10.1152/physrev.1968.48.3.534. [DOI] [PubMed] [Google Scholar]
  9. MCBURNEY J. J., MEIER H., HOAG W. G. DEVICE FOR MILKING MICE. J Lab Clin Med. 1964 Sep;64:485–487. [PubMed] [Google Scholar]
  10. Murphy R. A., Pantazis N. J., Arnason B. G., Young M. Secretion of a nerve growth factor by mouse neuroblastoma cells in culture. Proc Natl Acad Sci U S A. 1975 May;72(5):1895–1898. doi: 10.1073/pnas.72.5.1895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oger J., Arnason B. G., Pantazis N., Lehrich J., Young M. Synthesis of nerve growth factor by L and 3T3 cells in culture. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1554–1558. doi: 10.1073/pnas.71.4.1554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schwab M. E., Stöckel K., Thoenen H. Immunocytochemical localization of nerve growth factor (NGF) in the submandibular gland of adult mice by light and electron microscopy. Cell Tissue Res. 1976 Jun 28;169(3):289–299. doi: 10.1007/BF00219602. [DOI] [PubMed] [Google Scholar]
  13. Wallace L. J., Partlow L. M. alpha-Adrenergic regulation of secretion of mouse saliva rich in nerve growth factor. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4210–4214. doi: 10.1073/pnas.73.11.4210. [DOI] [PMC free article] [PubMed] [Google Scholar]

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