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. 1975 Jan;72(1):64–68. doi: 10.1073/pnas.72.1.64

Identification of a calcium-binding protein as a calcium-dependent regulator of brain adenylate cyclase.

C O Brostrom, Y C Huang, B M Breckenridge, D J Wolff
PMCID: PMC432241  PMID: 164029

Abstract

An activating factor of adenylate cyclase (EC 4.6.1.1) HAS BEEN OBTAINED FROM DETERGENT-DISPERSED PREPARATIONS OF PORCINE CEREBRAL CORTEX BY COLUMN CHROMATOGRAPHY ON ECTEOLA-cellulose. The factor was identified by acrylamide gel electrophoresis and by enzyme activation studies as the Ca2+-binding protein that regulates the activity of a brain cyclic nucleotide phosphodiesterase. This Ca2+-binding protein confers a Ca2+-dependent activation upon the adenylate cyclase, which is reversed by the subsequent addition of egta in excess of the free Ca2+. It is proposed that this Ca2+-dependent regulator controls enzymatic activities responsible for the synthesis of adenosine 3':5'-monophosphate and for the hydrolysis of guanosine 3':5'-monophosphate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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