Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Jan;72(1):229–230. doi: 10.1073/pnas.72.1.229

Demonstration of the rapid action of pure crystalline 1 alpha-hydroxy vitamin D3 and 1 alpha,25-dihydroxy vitamin D3 on intestinal calcium uptake.

E P Toffolon, M M Pechet, K Isselbacher
PMCID: PMC432276  PMID: 1054498

Abstract

The biological effects of crystalline 1alpha-hydroxyvitamin D3 and crystalline 1alpha,25-dihydroxyvitamin D3 have been compared on the intestinal uptake of calcium-45 by everted duodenal gut sacs from rachitic rats. Peak calcium-45 uptake was observed 1 hr after intravenous administration and both crystalline vitamin D2 analogs were of comparable potency. The rapid onset of calcium-45 uptake and the rapid attainment of maximal calcium-45 transport suggests a direct effect of these crystalline analogs on the mucosal membranes of the intestinal cell.

Full text

PDF
229

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams T. H., Wong R. G., Norman A. W. Studies on the mechanism of action of calciferol. II. Effects of the polyene antibiotic, filipin, on vitamin D-mediated calcium transport. J Biol Chem. 1970 Sep 10;245(17):4432–4442. [PubMed] [Google Scholar]
  2. Barton D. H., Hesse R. H., Pechet M. M., Rizzardo E. A convenient synthesis of 1 -hydroxy-vitamin D 3 . J Am Chem Soc. 1973 Apr 18;95(8):2748–2749. doi: 10.1021/ja00789a090. [DOI] [PubMed] [Google Scholar]
  3. Blunt J. W., DeLuca H. F., Schnoes H. K. 25-hydroxycholecalciferol. A biologically active metabolite of vitamin D3. Biochemistry. 1968 Oct;7(10):3317–3322. doi: 10.1021/bi00850a001. [DOI] [PubMed] [Google Scholar]
  4. Brumbaugh P. F., Haussler M. R. 1 Alpha,25-dihydroxycholecalciferol receptors in intestine. I. Association of 1 alpha,25-dihydroxycholecalciferol with intestinal mucosa chromatin. J Biol Chem. 1974 Feb 25;249(4):1251–1257. [PubMed] [Google Scholar]
  5. Chen T. C., DeLuca H. F. Receptors of 1,25-dikydroxycholecalciferol in rat intestine. J Biol Chem. 1973 Jul 25;248(14):4890–4895. [PubMed] [Google Scholar]
  6. DEMEL R. A., VAN DEENENL PENETRATION OF LIPID MONOLAYERS BY POLYENE ANTIBIOTICS. CORRELATION WITH SELECTIVE TOXICITY AND MODE OF ACTION. J Biol Chem. 1965 Jun;240:2749–2753. [PubMed] [Google Scholar]
  7. Fraser D. R., Kodicek E. Unique biosynthesis by kidney of a biological active vitamin D metabolite. Nature. 1970 Nov 21;228(5273):764–766. doi: 10.1038/228764a0. [DOI] [PubMed] [Google Scholar]
  8. Haussler M. R., Zerwekh J. E., Hesse R. H., Rizzardo E., Pechet M. M. Biological activity of 1alpha-hydroxycholecalciferol, a synthetic analog of the hormonal form of vitamin D3. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2248–2252. doi: 10.1073/pnas.70.8.2248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Holick M. F., Semmler E. J., Schnoes H. K., DeLuca H. F. 1 -Hydroxy derivative of vitamin D 3 : a highly potent analog of 1 ,25-dihydroxyvitamin D 3 . Science. 1973 Apr 15;180(4082):190–191. doi: 10.1126/science.180.4082.190. [DOI] [PubMed] [Google Scholar]
  10. Martin D. L., DeLuca H. F. Influence of sodium on calcium transport by the rat small intestine. Am J Physiol. 1969 Jun;216(6):1351–1359. doi: 10.1152/ajplegacy.1969.216.6.1351. [DOI] [PubMed] [Google Scholar]
  11. Norman A. W., Myrtle J. F., Midgett R. J., Nowicki H. G., Williams V., Popják G. 1,25-dihydroxycholecalciferol: identification of the proposed active form of vitamin D3 in the intestine. Science. 1971 Jul 2;173(3991):51–54. doi: 10.1126/science.173.3991.51. [DOI] [PubMed] [Google Scholar]
  12. SCHACHTER D., ROSEN S. M. Active transport of Ca45 by the small intestine and its dependence on vitamin D. Am J Physiol. 1959 Feb;196(2):357–362. doi: 10.1152/ajplegacy.1959.196.2.357. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES