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. 1975 Jan;72(1):323–327. doi: 10.1073/pnas.72.1.323

Competent Bacillus subtilis cultures synthesize a denatured DNA binding activity.

E Eisenstadt, R Lange, K Willecke
PMCID: PMC432297  PMID: 164019

Abstract

A protein activity has been detected in soluble extracts of competent cultures of bacillus subtilis which protects denatured DNA from nuclease solubilization. Both binding activity and competence development occur simultaneously but not coordinately, since the peak of binding activity appears 3 hr after the peak of competence. The binding activivity is not detected in physiologically noncompetent cultures, in an isogenic noncompetent cultures, in an isogenic noncompetent mutant grown through the competence regimen, or in early sporulating cells. The activity is included on Sephadex G-100 gel filtration columns but elutes in a broad nonhomogeneous peak. This binding activity may play a role in uptake of DNA and/or integration of exogenous DNA into the genome of competent B. subtilis cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham G. N., Scaletta C., Vaughan J. H. Modified diphenylamine reaction for increased sensitivity. Anal Biochem. 1972 Oct;49(2):547–549. doi: 10.1016/0003-2697(72)90460-5. [DOI] [PubMed] [Google Scholar]
  2. Alberts B. M., Frey L. T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA. Nature. 1970 Sep 26;227(5265):1313–1318. doi: 10.1038/2271313a0. [DOI] [PubMed] [Google Scholar]
  3. Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ayad S. R., Barker G. R. The integration of donor and recipient deoxyribonucleic acid during transformation of Bacillus subtilis. Biochem J. 1969 Jun;113(1):167–174. doi: 10.1042/bj1130167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BODMER W. F., GANESAN A. T. BIOCHEMICAL AND GENETIC STUDIES OF INTEGRATION AND RECOMBINATION IN BACILLUS SUBTILIS TRANSFORMATION. Genetics. 1964 Oct;50:717–738. doi: 10.1093/genetics/50.4.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. BRESLER S. E., KRENEVA R. A., KUSHEV V. V., MOSEVITSKII M. I. MOLECULAR MECHANISM OF GENETIC RECOMBINATION IN BACTERIAL TRANSFORMATION. Z Vererbungsl. 1964 Nov 11;95:288–297. doi: 10.1007/BF00897013. [DOI] [PubMed] [Google Scholar]
  7. Berkower I., Leis J., Hurwitz J. Isolation and characterization of an endonuclease from Escherichia coli specific for ribonucleic acid in ribonucleic acid-deoxyribonucleic acid hybrid structures. J Biol Chem. 1973 Sep 10;248(17):5914–5921. [PubMed] [Google Scholar]
  8. Cahn F. H., Fox M. S. Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients. J Bacteriol. 1968 Mar;95(3):867–875. doi: 10.1128/jb.95.3.867-875.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Davidoff-Abelson R., Dubnau D. Conditions affecting the isolation from transformed cells of Bacillus subtilis of high-molecular-weight single-stranded deoxyribonucleic acid of donor origin. J Bacteriol. 1973 Oct;116(1):146–153. doi: 10.1128/jb.116.1.146-153.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dubnau D., Davidoff-Abelson R. Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex. J Mol Biol. 1971 Mar 14;56(2):209–221. doi: 10.1016/0022-2836(71)90460-8. [DOI] [PubMed] [Google Scholar]
  11. Hotta Y., Stern H. A DNA-binding protein in meiotic cells of Lilium. Dev Biol. 1971 Sep;26(1):87–99. doi: 10.1016/0012-1606(71)90110-2. [DOI] [PubMed] [Google Scholar]
  12. Hotta Y., Stern H. Meiotic protein in spermatocytes of mammals. Nat New Biol. 1971 Nov 17;234(46):83–86. doi: 10.1038/newbio234083a0. [DOI] [PubMed] [Google Scholar]
  13. Huang W. M., Lehman I. R. On the exonuclease activity of phage T4 deoxyribonucleic acid polymerase. J Biol Chem. 1972 May 25;247(10):3139–3146. [PubMed] [Google Scholar]
  14. Michel J. F., Piechaud M., Schaeffer P. Constituvité vis-a-vis du nitrate de la nitrate-réductase chez les mutants asporogènes précoces de bacillus subtilis. Ann Inst Pasteur (Paris) 1970 Dec;119(6):711–718. [PubMed] [Google Scholar]
  15. Piechowska M., Fox M. S. Fate of transforming deoxyribonucleate in Bacillus subtilis. J Bacteriol. 1971 Nov;108(2):680–689. doi: 10.1128/jb.108.2.680-689.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schaller H., Nüsslein C., Bonhoeffer F. J., Kurz C., Nietzschmann I. Affinity chromatography of DNA-binding enzymes on single-stranded DNA-agarose columns. Eur J Biochem. 1972 Apr 24;26(4):474–481. doi: 10.1111/j.1432-1033.1972.tb01789.x. [DOI] [PubMed] [Google Scholar]
  17. VENEMA G., PRITCHARD R. H., VENEMA-SCHROEDER T. FATE OF TRANSFORMING DEOXYRIBONUCLEIC ACID IN BACILLUS SUBTILIS. J Bacteriol. 1965 May;89:1250–1255. doi: 10.1128/jb.89.5.1250-1255.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

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