Fig.4. Down-regulating c-Jun decreases expression of Brf1 and Pol III genes.
(A and C) Decrease in c-Jun expression MCF-7 cells were treated as described in Fig.2. The cellular levels of c-Jun protein (A) and mRNA (C) were determined. Tam reduces the expression of c-Jun increased by alcohol. (B, D, E and F); c-jun siRNA MCF-7 cells were transfected with mismatch RNA (mmRNA) or c-Jun siRNA for 48 h. The cells were treated as described in A. RT-qPCR and immunoblot analysis was performed to determine cellular levels of c-Jun (B and D), ERα (B and E), and Brf1 (B and F); (G-H) Pol III gene transcription The cells were treated as described above. Total RNAs were extracted from these cells and RT-qPCR was performed to measure the amounts of pre-tRNALeu (G) and 5S rRNA (H). The fold change was calculated by normalizing to the amount of GAPDH mRNA. The bars represent Mean ± SE of at least three independent determinations.