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. 1975 Feb;72(2):459–463. doi: 10.1073/pnas.72.2.459

Antibody against tuberlin: the specific visualization of cytoplasmic microtubules in tissue culture cells.

K Weber, R Pollack, T Bibring
PMCID: PMC432331  PMID: 804694

Abstract

Cytoplasmic microtubules in tissue culture cells can be directly visualized by immunofluorescence microscopy. Antibody against tubulin from the outer doublets of sea urchin sperm flagella decorates a network of fine cytoplasmic fibers in a variety of cell lines of human, monkey, rat, mouse, and chicken origin. These fibers are separate and of uniform thickness and are seen throughout the cytoplasm. The fibers disappear either in a medium containing colchicine or after subjection of the cells to low temperature. The same treatments do not destroy the microfilamentous structures that are visualized by means of antibody against actin. When tryspin-treated enucleated cells are replated and then stained with antibody against tubulin, the fibers can be seen to traverse the entire enucleated cytoplasm.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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