Abstract
An in vitro assay for measuring the activity of the phage lambda A-gene product has been developed. The assay is based on the observation that A-donor extracts complement A minus extracts for packaging of exogenous immature lambda DNA into phage particles. A partial purification of the A-gene product activity using this assay is presented. A method is suggested by which this A protein-dependent in vitro system might be manipulated to analyze the mechanism of reforming the lambda cohesive termini during chromosome assimilation into phage precursors.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Becker A. An endodeoxyribonuclease induced in E. coli by infection with lambda-pbio transducing phages. Biochem Biophys Res Commun. 1970 Oct 9;41(1):63–70. doi: 10.1016/0006-291x(70)90469-9. [DOI] [PubMed] [Google Scholar]
- Carter B. J., Shaw B. D., Smith M. G. Two stages in the replication of bacteriophage lambda DNA. Biochim Biophys Acta. 1969 Dec 16;195(2):494–505. doi: 10.1016/0005-2787(69)90656-x. [DOI] [PubMed] [Google Scholar]
- Dove W. F. Action of the lambda chromosome. I. Control of functions late in bacteriophage development. J Mol Biol. 1966 Aug;19(1):187–201. doi: 10.1016/s0022-2836(66)80060-8. [DOI] [PubMed] [Google Scholar]
- Emmons S. W. Bacteriophage lambda derivatives carrying two copies of the cohesive end site. J Mol Biol. 1974 Mar 15;83(4):511–525. doi: 10.1016/0022-2836(74)90511-7. [DOI] [PubMed] [Google Scholar]
- Enquist L. W., Skalka A. Replication of bacteriophage lambda DNA dependent on the function of host and viral genes. I. Interaction of red, gam and rec. J Mol Biol. 1973 Apr 5;75(2):185–212. doi: 10.1016/0022-2836(73)90016-8. [DOI] [PubMed] [Google Scholar]
- Gellert M. Formation of covalent circles of lambda DNA by E. coli extracts. Proc Natl Acad Sci U S A. 1967 Jan;57(1):148–155. doi: 10.1073/pnas.57.1.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Georgopoulos C. P., Hendrix R. W., Casjens S. R., Kaiser A. D. Host participation in bacteriophage lambda head assembly. J Mol Biol. 1973 May 5;76(1):45–60. doi: 10.1016/0022-2836(73)90080-6. [DOI] [PubMed] [Google Scholar]
- HERSHEY A. D., BURGI E. COMPLEMENTARY STRUCTURE OF INTERACTING SITES AT THE ENDS OF LAMBDA DNA MOLECULES. Proc Natl Acad Sci U S A. 1965 Feb;53:325–328. doi: 10.1073/pnas.53.2.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hohn B., Hohn T. Activity of empty, headlike particles for packaging of DNA of bacteriophage lambda in vitro. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2372–2376. doi: 10.1073/pnas.71.6.2372. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaiser D., Masuda T. In vitro assembly of bacteriophage Lambda heads. Proc Natl Acad Sci U S A. 1973 Jan;70(1):260–264. doi: 10.1073/pnas.70.1.260. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Little J. W., Zimmerman S. B., Oshinsky C. K., Gellert M. Enzymatic joining of DNA strands, II. An enzyme-adenylate intermediate in the dpn-dependent DNA ligase reaction. Proc Natl Acad Sci U S A. 1967 Nov;58(5):2004–2011. doi: 10.1073/pnas.58.5.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mackinlay A. G., Kaiser A. D. DNA replication in head mutants of bacteriophage lambda. J Mol Biol. 1969 Feb 14;39(3):679–683. doi: 10.1016/0022-2836(69)90155-7. [DOI] [PubMed] [Google Scholar]
- McClure S. C., Gold M. Intermediates in the maturation of bacteriophage lambda DNA. Virology. 1973 Jul;54(1):19–27. doi: 10.1016/0042-6822(73)90110-4. [DOI] [PubMed] [Google Scholar]
- McClure S. C., MacHattie L., Gold M. A sedimentation analysis of DNA found in Escherichia coli infected with phage lambda mutants. Virology. 1973 Jul;54(1):1–18. doi: 10.1016/0042-6822(73)90109-8. [DOI] [PubMed] [Google Scholar]
- Modrich P., Lehman I. R. Enzymatic joining of polynucleotides. IX. A simple and rapid assay of polynucleotide joining (ligase) activity by measurement of circle formation from linear deoxyadenylate-deoxythymidylate copolymer. J Biol Chem. 1970 Jul 25;245(14):3626–3631. [PubMed] [Google Scholar]
- Mousset S., Thomas R. Ter, a function which generates the ends of the mature lambda chromosome. Nature. 1969 Jan 18;221(5177):242–244. doi: 10.1038/221242a0. [DOI] [PubMed] [Google Scholar]
- Murialdo H., Siminovitch L. The morphogenesis of bacteriophage lambda. IV. Identification of gene products and control of the expression of the morphogenetic information. Virology. 1972 Jun;48(3):785–823. doi: 10.1016/0042-6822(72)90162-6. [DOI] [PubMed] [Google Scholar]
- Sadowski P., McGeer A., Becker A. Terminal cross-linking of DNA catalyzed by an enzyme system containing DNA ligase, DNA polymerase, and exonuclease of bacteriophage T7. Can J Biochem. 1974 Jun;52(6):525–535. doi: 10.1139/o74-077. [DOI] [PubMed] [Google Scholar]
- Skalka A., Poonian M., Bartl P. Concatemers in DNA replication: electron microscopic studies of partially denatured intracellular lambda DNA. J Mol Biol. 1972 Mar 14;64(3):541–550. doi: 10.1016/0022-2836(72)90081-2. [DOI] [PubMed] [Google Scholar]
- Syvanen M. In vitro genetic recombination of bacteriophage lambda. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2496–2499. doi: 10.1073/pnas.71.6.2496. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wake R. G., Kaiser A. D., Inman R. B. Isolation and structure of phage lambda head-mutant DNA. J Mol Biol. 1972 Mar 14;64(3):519–540. doi: 10.1016/0022-2836(72)90080-0. [DOI] [PubMed] [Google Scholar]
- Wang J. C., Kaiser A. D. Evidence that the cohesive ends of mature lambda DNA are generated by the gene A product. Nat New Biol. 1973 Jan 3;241(105):16–17. doi: 10.1038/newbio241016a0. [DOI] [PubMed] [Google Scholar]
- Wu R., Taylor E. Nucleotide sequence analysis of DNA. II. Complete nucleotide sequence of the cohesive ends of bacteriophage lambda DNA. J Mol Biol. 1971 May 14;57(3):491–511. doi: 10.1016/0022-2836(71)90105-7. [DOI] [PubMed] [Google Scholar]