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. 1975 Feb;72(2):697–700. doi: 10.1073/pnas.72.2.697

Role of amino groups in formation of human lymphocyte-xenogeneic erythrocyte rosettes; a proposed mechanism for antigen recognition.

P Lalezari, S L Nehlsen, J Novodoff, I Lalezari
PMCID: PMC432382  PMID: 235762

Abstract

Formation of coulombic and possible hydrogen bonds between amino groups on human lymphocytes and negatively charged sites on sheep erythrocytes is involved in rosette formation. Supportive evidence includes rosette inhibition by chemical binding of lymphocyte membrane amino groups, and the results of changing the pH, ionic concentration, and temperature of the reaction. Although the possibility has not been excluded that the amino group dependence of this reaction is related to the property of certain proteins attached to the T-cell (thymus processed) surface, it is suggested that this dependence may be related to a charge pattern recognition in the form of "codes" present on the T-cell membrane. It is speculated that this type of recognition may be a contributory mechanism in the initiation of the T-cell-dependent immune response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Argyris B. F., Haritou H., Cooney A. Density gradient fractionation of mouse lymphoid tissues. I. Plaque-forming and rosette-forming cells in normal, sensitized and tolerant spleen. Cell Immunol. 1972 Jan;3(1):101–112. doi: 10.1016/0008-8749(72)90230-4. [DOI] [PubMed] [Google Scholar]
  2. Bach J. F., Dardenne M. Antigen recognition by T lymphocytes. Thymus and marrow dependence of spontaneous rosette forming cells in the mouse. Cell Immunol. 1972 Jan;3(1):1–10. doi: 10.1016/0008-8749(72)90220-1. [DOI] [PubMed] [Google Scholar]
  3. Bach J. F., Dardenne M. pH and rosette formation. Lancet. 1972 Mar 18;1(7751):633–633. doi: 10.1016/s0140-6736(72)90429-1. [DOI] [PubMed] [Google Scholar]
  4. Brondz B. D., Ievleva E. S., Vornakova G. N. Vliianie antitel protiv gamma-globulina na tsitotoksicheskuiu aktivnost' immunykh limfotsitov v kulture allogennykh kletok. Biull Eksp Biol Med. 1968 Dec;66(12):73–77. [PubMed] [Google Scholar]
  5. Brondz B. D. Lymphocyte receptors and mechanisms of in vitro cell-mediated immune reactions. Transplant Rev. 1972;10:112–151. doi: 10.1111/j.1600-065x.1972.tb01541.x. [DOI] [PubMed] [Google Scholar]
  6. CHEN C. C., GROSSBERG A. L., PRESSMAN D. Effect of cyanate on several anti-hapten antibodies: evidence for the presence of an amino group in the site of anti-p-azobenzenearsonate antibody. Biochemistry. 1962 Nov;1:1025–1030. doi: 10.1021/bi00912a012. [DOI] [PubMed] [Google Scholar]
  7. Crone M., Koch C., Simonsen M. The elusive T cell receptor. Transplant Rev. 1972;10:36–56. doi: 10.1111/j.1600-065x.1972.tb01538.x. [DOI] [PubMed] [Google Scholar]
  8. Feldmann M., Cone R. E., Marchalonis J. J. Cell interactions in the immune response in vitro. VI. Mediation by T cell surface monomeric IgM. Cell Immunol. 1973 Oct;9(1):1–11. doi: 10.1016/0008-8749(73)90162-7. [DOI] [PubMed] [Google Scholar]
  9. Gorczynski R. M., Miller R. G., Phillips R. A. In vivo requirement for a radiation-resistant cells in the immune response to sheep erythrocytes. J Exp Med. 1971 Nov 1;134(5):1201–1221. doi: 10.1084/jem.134.5.1201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hogg N. M., Greaves M. F. Antigen-binding thymus-derived lymphocytes. I. Rapid method for isolation of theta-positive antigen-stimulated cells. Immunology. 1972 Jun;22(6):959–965. [PMC free article] [PubMed] [Google Scholar]
  11. Hunter P., Munro A., McConnell I. Properties of educated T cells for rosette formation and cooperation with B cells. Nat New Biol. 1972 Mar 15;236(63):52–53. doi: 10.1038/newbio236052a0. [DOI] [PubMed] [Google Scholar]
  12. Koch C., Nielsen H. E. Effect of anti light-chain antibodies on rat leukocytes in vitro. Scand J Immunol. 1973;2(1):1–8. doi: 10.1111/j.1365-3083.1973.tb02009.x. [DOI] [PubMed] [Google Scholar]
  13. Lalezari P. A mew method for detection of red blood cell antibodies. Transfusion. 1968 Nov-Dec;8(6):372–380. doi: 10.1111/j.1537-2995.1968.tb02439.x. [DOI] [PubMed] [Google Scholar]
  14. Marchalonis J. J., Atwell J. L., Cone R. E. Isolation of surface immunoglobulin from lymphocytes from human and murine thymus. Nat New Biol. 1972 Feb 23;235(60):240–242. doi: 10.1038/newbio235240a0. [DOI] [PubMed] [Google Scholar]
  15. Mäkelä O. Analogies between lymphocyte receptors and the resulting humoral antibodies. Transplant Rev. 1970;5:3–18. [PubMed] [Google Scholar]
  16. Parrott D. M., de Sousa M. A. Changes in the thymus-dependent areas of lymph nodes after immunological stimulation. Nature. 1966 Dec 17;212(5068):1316–1317. doi: 10.1038/2121316a0. [DOI] [PubMed] [Google Scholar]
  17. Roberts C. I., Brandriss M. W., Vaughan J. H. Failure of immunocytoadherence to demonstrate delayed hypersensitivity. J Immunol. 1971 Apr;106(4):1056–1064. [PubMed] [Google Scholar]
  18. Takahashi T., Old L. J., McIntire K. R., Boyse E. A. Immunoglobulin and other surface antigens of cells of the immune system. J Exp Med. 1971 Oct 1;134(4):815–832. doi: 10.1084/jem.134.4.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Warner N. L., Byrt P., Ada G. L. Blocking of the lymphocyte antigen receptor site with anti-immunoglobulin sera in vitro. Nature. 1970 Jun 6;226(5249):942–943. doi: 10.1038/226942a0. [DOI] [PubMed] [Google Scholar]
  20. Wilson J. D., Miller J. F. T and B rosette-forming cells. Eur J Immunol. 1971 Dec;1(6):501–503. doi: 10.1002/eji.1830010622. [DOI] [PubMed] [Google Scholar]

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