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. 1975 Feb;72(2):701–705. doi: 10.1073/pnas.72.2.701

Translation in vitro of rat brain messenger RNA coding for tubulin and actin.

I Gozes, H Schmitt, U Z Littauer
PMCID: PMC432383  PMID: 1054847

Abstract

A partially purified fraction of poly(a)-rich brain mRNA coding for tubulin and actin was obtained by stepwise elution from an oligo(deoxythymidylate)-cellulose column and was efficiently translated in a wheat-germ cell-free system. The newly synthesized tubulin and actin migrated along with the authentic proteins on sodium dodecyl sulfate polyacrylamide gels and on sodium dodecyl sulfate-urea polyacrylamide gels, where tubulin alpha- and beta-subunits are separated. The two proteins synthesized in vitro were found to be biologically active; they could be induced to polymerize and were both precipitated by vinblastine. In addition, specific binding of tubulin to an affinity column of colchicine-Sepharose and actin to myosin were demonstrated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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