Figure 3. Respiratory tract influenza-specific IgG antibody levels are lower in infant animals.
The amount of influenza-specific IgG, IgM, and IgA antibody in the tracheal wash (d8 and d14 p.i.) and/or BAL (d14 p.i.) was determined. IgA could not be assessed in tracheal washes due to limiting sample in the infants. Threshold dilution was defined as the dilution at which the influenza-specific OD reading (OD from influenza coated wells – OD from uncoated wells) reached three times the assay background. Significance at each timepoint was determined using a Mann Whitney test. * p≤0.05.