Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Mar;72(3):829–833. doi: 10.1073/pnas.72.3.829

Replicative intermediates of colicin E1 plasmid DNA in minicells.

A Oka, J Inselburg
PMCID: PMC432413  PMID: 1093172

Abstract

Pulse-labeled colicin E1 plasmid (Col E1) DNA in minicells was examined to characterize replicating molecules. Replication of Col E1 DNA principally occurred in covalently closed molecules and involved the synthesis of variable length single-stranded DNA fragments that were dissociable from the template DNA and ultimately incorporated into the completely replicated molecules. RNA was found to be associated with some of these newly synthesized fragments. Replicating molecules containing different size displacement loops were observed by electron microscopy.

Full text

PDF

Images in this article

832Image
on p.832

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bazaral M., Helinski D. R. Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513–3520. doi: 10.1021/bi00850a028. [DOI] [PubMed] [Google Scholar]
  3. Blair D. G., Sherratt D. J., Clewell D. B., Helinski D. R. Isolation of supercoiled colicinogenic factor E 1 DNA sensitive to ribonuclease and alkali. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2518–2522. doi: 10.1073/pnas.69.9.2518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fox R. M., Mendelsohn J., Barbosa E., Goulian M. RNA in nascent DNA from cultured human lymphocytes. Nat New Biol. 1973 Oct 24;245(147):234–237. doi: 10.1038/newbio245234a0. [DOI] [PubMed] [Google Scholar]
  5. Fuke M., Inselburg J. Electron microscopic studies of replicating and catenated colicin factor E1 DNA isolated from minicells (DNA replication). Proc Natl Acad Sci U S A. 1972 Jan;69(1):89–92. doi: 10.1073/pnas.69.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Inselburg J. Colicin factor DNA: a single non-homologous region in Col E2-E3 heteroduplex molecules. Nat New Biol. 1973 Feb 21;241(112):234–237. doi: 10.1038/newbio241234a0. [DOI] [PubMed] [Google Scholar]
  7. Inselburg J., Fuke M. Isolation of catenated and replicating DNA molecules of colicin factor E1 from minicells. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2839–2842. doi: 10.1073/pnas.68.11.2839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Inselburg J., Fuke M. Replicating DNA: structure of colicin factor E1. Science. 1970 Aug 7;169(3945):590–592. doi: 10.1126/science.169.3945.590. [DOI] [PubMed] [Google Scholar]
  9. Inselburg J. Replication of colicin E1 plasmid DNA in minicells from a unique replication initiation site. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2256–2259. doi: 10.1073/pnas.71.6.2256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Inselburg J. Segregation into and replication of plasmid deoxyribonucleic acid in chromosomeless segregants of Escherichia coli. J Bacteriol. 1970 Jun;102(3):642–647. doi: 10.1128/jb.102.3.642-647.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jaenisch R., Mayer A., Levine A. Replicating SV40 molecules containing closed circular template DNA strands. Nat New Biol. 1971 Sep 15;233(37):72–75. doi: 10.1038/newbio233072a0. [DOI] [PubMed] [Google Scholar]
  12. Kasamatsu H., Robberson D. L., Vinograd J. A novel closed-circular mitochondrial DNA with properties of a replicating intermediate. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2252–2257. doi: 10.1073/pnas.68.9.2252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  14. Sakakibara Y., Tomizawa J. I. Replication of colicin E1 plasmid DNA in cell extracts. Proc Natl Acad Sci U S A. 1974 Mar;71(3):802–806. doi: 10.1073/pnas.71.3.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sakakibara Y., Tomizawa J. Replication of colicin E1 plasmid DNA in cell extracts. II. Selective synthesis of early replicative intermediates. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1403–1407. doi: 10.1073/pnas.71.4.1403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sato S., Ariake S., Saito M., Sugimura T. RNA bound to nascent DNA in Ehrlich ascites tumor cells. Biochem Biophys Res Commun. 1972 Nov 1;49(3):827–834. doi: 10.1016/0006-291x(72)90485-8. [DOI] [PubMed] [Google Scholar]
  17. Schekman R., Wickner W., Westergaard O., Brutlag D., Geider K., Bertsch L. L., Kornberg A. Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691–2695. doi: 10.1073/pnas.69.9.2691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sugino A., Hirose S., Okazaki R. RNA-linked nascent DNA fragments in Escherichia coli. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1863–1867. doi: 10.1073/pnas.69.7.1863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tomizawa J., Sakakibara Y., Kakefuda T. Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260–2264. doi: 10.1073/pnas.71.6.2260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Waqar M. A., Huberman J. A. Evidence for the attachment of RNA to pulse-labeled DNA in the slime mold, Physarum polycephalum. Biochem Biophys Res Commun. 1973 Mar 5;51(1):174–180. doi: 10.1016/0006-291x(73)90524-x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES