Abstract
Pseudomonas phaseolicola infected with bacteriophage phi 6 synthesized all three viral double-stranded RNA segments, three single-stranded RNAs, and three replicative intermediate-like RNAs in the presence of rifampin. The single-stranded RNA intermediates sedimented and electrophoresed along with melted viral double-stranded RNA, annealed with melted viral double-stranded RNA, and were transient in nature. The relative amounts of the single-stranded RNA intermediates varied during the infection cycle and were altered in the presence of chloramphenicol. The replicative intermediate-like RNAs sedimented faster than double-stranded RNA, failed to enter 2.5% polyacrylamide gels, eluted with double-stranded RNA from a CF-11 cellulose column, were precipitated with single-stranded RNA in 2 M LiC1, and yielded three genome-size pieces of double-stranded RNA upon digestion with RNase. These results are consistent with the hypothesis that complementary strands of the phi 6 double-stranded RNAs are synthesized asynchronously during the infection cycle.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brakke M. K., Van Pelt N. Linear-log sucrose gradients for estimating sedimentation coefficients of plant viruses and nucleic acids. Anal Biochem. 1970 Nov;38(1):56–64. doi: 10.1016/0003-2697(70)90155-7. [DOI] [PubMed] [Google Scholar]
- Franklin R. M. Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504–1511. doi: 10.1073/pnas.55.6.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nonoyama M., Millward S., Graham A. F. Control of transcription of the reovirus genome. Nucleic Acids Res. 1974 Mar;1(3):373–385. doi: 10.1093/nar/1.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schonberg M., Silverstein S. C., Levin D. H., Acs G. Asynchronous synthesis of the complementary strands of the reovirus genome. Proc Natl Acad Sci U S A. 1971 Feb;68(2):505–508. doi: 10.1073/pnas.68.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Semancik J. S., Vidaver A. K., Van Etten J. L. Characterization of segmented double-helical RNA from bacteriophage phi6. J Mol Biol. 1973 Aug 25;78(4):617–625. doi: 10.1016/0022-2836(73)90283-0. [DOI] [PubMed] [Google Scholar]
- Thach S. S., Thach R. E. Mechanism of viral replication. I. Structure of replication complexes of R17 bacteriophage. J Mol Biol. 1973 Dec 15;81(3):367–380. doi: 10.1016/0022-2836(73)90147-2. [DOI] [PubMed] [Google Scholar]
- Van Etten J. L., Vidaver A. K., Koski R. K., Burnett J. P. Base composition and hybridization studies of the three double-stranded RNA segments of bacteriophage phi 6. J Virol. 1974 Jun;13(6):1254–1262. doi: 10.1128/jvi.13.6.1254-1262.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Etten J. L., Vidaver A. K., Koski R. K., Semancik J. S. RNA polymerase activity associated with bacteriophage phi 6. J Virol. 1973 Sep;12(3):464–471. doi: 10.1128/jvi.12.3.464-471.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vidaver A. K., Koski R. K., Van Etten J. L. Bacteriophage phi6: a Lipid-Containing Virus of Pseudomonas phaseolicola. J Virol. 1973 May;11(5):799–805. doi: 10.1128/jvi.11.5.799-805.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vidaver A. K., Schuster M. L. Characterization of Xanthomonas phaseoli Bacteriophages. J Virol. 1969 Sep;4(3):300–308. doi: 10.1128/jvi.4.3.300-308.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watanabe Y., Kudo H., Graham A. F. Selective inhibition of reovirus ribonucleic acid synthesis by cycloheximide. J Virol. 1967 Feb;1(1):36–44. doi: 10.1128/jvi.1.1.36-44.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]