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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Mar;72(3):911–915. doi: 10.1073/pnas.72.3.911

Regulation of RNA polymerase synthesis in Escherichia coli: a mutant unable to synthesize the enzyme at 43 degrees.

M P Oeschger, M K Berlyn
PMCID: PMC432431  PMID: 1093173

Abstract

We report the isolation of a mutant of E. coli in which the capacity to synthesize RNA polymerase (EC 2.7.7.6) (the beta and beta' subunits) is rapidly lost at 43 degrees. The mutation has no effect on the stability or activity of the polymerase itself. The mutation is recessive and is closely linked to the rif locus (the structural gene for the beta subunit). Using strains carrying the mutation, we have shown that polymerase is present in excess in rapidly growing E. coli cells.

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Selected References

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  1. Bass I. A., Gorlenko Z. M., Dmitriev A. D., Ilyina T. S., Ovadis M. I. Content of RNA polymerase in haploid and merodiploid strains of Escherichia coli. Mol Gen Genet. 1973 Nov 12;126(3):247–254. doi: 10.1007/BF00267535. [DOI] [PubMed] [Google Scholar]
  2. Bremer H., Berry L., Dennis P. P. Regulation of ribonucleic acid synthesis in Escherichia coli B-r: an analysis of a shift-up. II. Fraction of RNA polymerase engaged in the synthesis of stable RNA at different steady-state growth rates. J Mol Biol. 1973 Mar 25;75(1):161–179. doi: 10.1016/0022-2836(73)90536-6. [DOI] [PubMed] [Google Scholar]
  3. Burgess R. R. RNA polymerase. Annu Rev Biochem. 1971;40:711–740. doi: 10.1146/annurev.bi.40.070171.003431. [DOI] [PubMed] [Google Scholar]
  4. Burgess R. R. Separation and characterization of the subunits of ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6168–6176. [PubMed] [Google Scholar]
  5. Chamberlin M. J. The selectivity of transcription. Annu Rev Biochem. 1974;43(0):721–775. doi: 10.1146/annurev.bi.43.070174.003445. [DOI] [PubMed] [Google Scholar]
  6. Cronan J. E., Jr, Silbert D. F., Wulff D. L. Mapping of the fabA locus for unsaturated fatty acid biosynthesis in Escherichia coli. J Bacteriol. 1972 Oct;112(1):206–211. doi: 10.1128/jb.112.1.206-211.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dalbow D. G. Synthesis of RNA polymerase in Escherichia coli B-r growing at different rates. J Mol Biol. 1973 Mar 25;75(1):181–184. doi: 10.1016/0022-2836(73)90537-8. [DOI] [PubMed] [Google Scholar]
  8. Duberstein R., Oeschger M. P. Growth of bacteriophage H on male and female strains of Escherichia coli. J Virol. 1973 Mar;11(3):460–463. doi: 10.1128/jvi.11.3.460-463.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Errington L., Glass R. E., Hayward R. S., Scaife J. G. Structure and orientation of an RNA polymerase operon in Escherichia coli. Nature. 1974 Jun 7;249(457):519–522. doi: 10.1038/249519a0. [DOI] [PubMed] [Google Scholar]
  10. Guerola N., Ingraham J. L., Cerdá-Olmedo E. Induction of closely linked multiple mutations by nitrosoguanidine. Nat New Biol. 1971 Mar 24;230(12):122–125. doi: 10.1038/newbio230122a0. [DOI] [PubMed] [Google Scholar]
  11. Hayward R. S., Austin S. J., Scaife J. G. The effect of gene dosage on the synthesis and stability of RNA polymerase subunits in Escherichia coli. Mol Gen Genet. 1974;131(2):173–180. doi: 10.1007/BF00266152. [DOI] [PubMed] [Google Scholar]
  12. Hayward R. S., Tittawella I. P., Scaife J. G. Evidence for specific control of RNA polymerase synthesis in Escherichia coli. Nat New Biol. 1973 May 2;243(122):6–9. [PubMed] [Google Scholar]
  13. Heil A., Zillig W. Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription. FEBS Lett. 1970 Dec;11(3):165–168. doi: 10.1016/0014-5793(70)80519-1. [DOI] [PubMed] [Google Scholar]
  14. Hill C. W., Combriato G. Genetic duplications induced at very high frequency by ultraviolet irradiation in Escherichia coli. Mol Gen Genet. 1973 Dec 31;127(3):197–214. doi: 10.1007/BF00333760. [DOI] [PubMed] [Google Scholar]
  15. Iwakura Y., Ito K., Ishihama A. Biosynthesis of RNA polymerase in Escherichia coli. I. Control of RNA polymerase content at various growth rates. Mol Gen Genet. 1974;133(1):1–23. doi: 10.1007/BF00268673. [DOI] [PubMed] [Google Scholar]
  16. Kirschbaum J. B. Regulation of subunit synthesis of Escherichia coli RNA polymerase. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2651–2655. doi: 10.1073/pnas.70.9.2651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kirschbaum J. B., Scaife J. Evidence for a lambda transducing phage carrying the genes for the beta and beta' subunits of Escherichia coli RNA polymerase. Mol Gen Genet. 1974;132(3):193–201. doi: 10.1007/BF00269392. [DOI] [PubMed] [Google Scholar]
  18. Losick R. In vitro transcription. Annu Rev Biochem. 1972;41:409–446. doi: 10.1146/annurev.bi.41.070172.002205. [DOI] [PubMed] [Google Scholar]
  19. Low B. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J Bacteriol. 1973 Feb;113(2):798–812. doi: 10.1128/jb.113.2.798-812.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Matzura H., Hansen B. S., Zeuthen J. Biosynthesis of the beta and beta' subunits of RNA polymerase in Escherichia coli. J Mol Biol. 1973 Feb 15;74(1):9–20. doi: 10.1016/0022-2836(73)90350-1. [DOI] [PubMed] [Google Scholar]
  22. Matzura H. Joint propagation of the and ' subunits of RNA polymerase in Escherichia coli. Nat New Biol. 1973 Jun 27;243(130):262–264. doi: 10.1038/newbio243262a0. [DOI] [PubMed] [Google Scholar]
  23. Matzura H., Molin S., Maaloe O. Sequential biosynthesis of the and ' subunits of the DNA-dependent RNA polymerase from Escherichia coli. J Mol Biol. 1971 Jul 14;59(1):17–25. doi: 10.1016/0022-2836(71)90410-4. [DOI] [PubMed] [Google Scholar]
  24. Morse D. E., Mosteller R. D., Yanofsky C. Dynamics of synthesis, translation, and degradation of trp operon messenger RNA in E. coli. Cold Spring Harb Symp Quant Biol. 1969;34:725–740. doi: 10.1101/sqb.1969.034.01.082. [DOI] [PubMed] [Google Scholar]
  25. Oeschger M. P., Berlyn M. K. A simple procedure for localized mutagenesis using nitrosoguanidine. Mol Gen Genet. 1974;134(1):77–83. doi: 10.1007/BF00332814. [DOI] [PubMed] [Google Scholar]
  26. Stadler D., Kariya B. Marker effects in the genetic transduction of tryptophan mutants of E. coli. Genetics. 1973 Nov;75(3):423–439. doi: 10.1093/genetics/75.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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