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. 1975 Mar;72(3):1021–1025. doi: 10.1073/pnas.72.3.1021

Interaction of poly(A) and mRNA with eukaryotic initiator met-tRNA-f binding factor: identification of this activity on reticulocyte ribonucleic acid protein particles.

J G Hellerman, D A Shafritz
PMCID: PMC432456  PMID: 1055361

Abstract

Messenger ribonucleoprotein particles (mRNPs) have been isolated from rabbit reticulocyte polysomes. One of the proteins of the mRNP complex has many properties of a specific eukaryotic initiation factor, the soluble Met-tRNA-f binding protein. A purified preparation of this factor, in addition to binding Met-tRNA-f, binds poly(A) and globin mRNA. The binding of these substrates is greater than that obtained with other natural or artificial polyribonucleotides. The mRNP fraction also binds poly(A) and Met-tRNA-f. Since the factor which binds initiator tRNA also binds poly(A) and mRNA, and this activity can be found on mRNPs, there may be a relationship between initiator tRNA binding and messenger binding in early events of eukaryotic initiation.

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Selected References

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