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. 1975 Mar;72(3):1031–1035. doi: 10.1073/pnas.72.3.1031

Host induced alteration of avian sarcoma virus B-77 genome.

M Shoyab, P D Markham, M A Baluda
PMCID: PMC432458  PMID: 165481

Abstract

The genome of an avian oncornavirus was altered after infection of a heterologous host. This was studied with avian sarcoma virus B-77 in duck embryonic fibroblasts (DEF) and chicken embryonic fibroblasts (CEF). To detect alteration of the viral genome, we hybridized 35S B-77 RNA with normal duck DNA by either one of two techniques:when viral RNA was in excess and when DNA was in excess. The RNA of B-77 passaged only in gs minus chf minus CEF does not have homology with duck DNA. However, after several passages of B-77 through DEF the viral genome acquired duck specific RNA sequences. After 4 and 10 passages, B-77 RNA acquired 2.2 and 6.6%, respectively, complementarity to normal duck DNA. The duck specific RNA sequences were found to be covalently linked to the B-77 RNA genome. Also, the host specific sequences acquired by the virus appear to be from a region of the duck DNA which is repeated four to six times per cell. After 5 back passages in CEF some of the duck specific RNA sequences in the viral genome were lost.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ali M., Baluda M. A. Synthesis of avian oncornavirus DNA in infected chicken cells. J Virol. 1974 May;13(5):1005–1013. doi: 10.1128/jvi.13.5.1005-1013.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Altaner C., Svec F. Virus production in rat tumors induced by chicken sarcoma virus. J Natl Cancer Inst. 1966 Dec;37(6):745–752. [PubMed] [Google Scholar]
  3. Altaner C., Temin H. M. Carcinogenesis by RNA sarcoma viruses. XII. A quantitative study of infection of rat cells in vitro by avian sarcoma viruses. Virology. 1970 Jan;40(1):118–134. doi: 10.1016/0042-6822(70)90384-3. [DOI] [PubMed] [Google Scholar]
  4. Baluda M. A., Nayak D. P. Incorporation of precursors into ribonucleic acid, protein, glycoprotein, and lipoprotein of avian myeloblastosis virions. J Virol. 1969 Nov;4(5):554–566. doi: 10.1128/jvi.4.5.554-566.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Baluda M. A. Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses. Proc Natl Acad Sci U S A. 1972 Mar;69(3):576–580. doi: 10.1073/pnas.69.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bank A., Terada M., Metafora S., Dow L., Marks P. A. In vitro synthesis of DNA components of human genes for globins. Nat New Biol. 1972 Feb 9;235(58):167–169. doi: 10.1038/newbio235167a0. [DOI] [PubMed] [Google Scholar]
  7. Evans R. M., Baluda M. A., Shoyab M. Differences between the integration of avian myeloblastosis virus DNA in leukemic cells and of endogenous viral DNA in normal chicken cells. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3152–3156. doi: 10.1073/pnas.71.8.3152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hanafusa H., Hanafusa T. Determining factor in the capacity of Rous sarcoma virus to induce tumors in mammals. Proc Natl Acad Sci U S A. 1966 Mar;55(3):532–538. doi: 10.1073/pnas.55.3.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hanafusa T., Hanafusa H., Miyamoto T., Fleissner E. Existence and expression of tumor virus genes in chick embryo cells. Virology. 1972 Feb;47(2):475–482. doi: 10.1016/0042-6822(72)90283-8. [DOI] [PubMed] [Google Scholar]
  10. Ikawa Y., Ross J., Leder P. An association between globin messenger RNA and 60S RNA derived from Friend leukemia virus. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1154–1158. doi: 10.1073/pnas.71.4.1154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kryukova I. N., Obukh I. B., Tot F. Characteristics of variants of Rous sarcoma virus (RSV) isolated from mouse RSV tumors. J Natl Cancer Inst. 1970 Jul;45(1):49–57. [PubMed] [Google Scholar]
  12. Linné T., Oberg B., Philipson L. RNA ligase activity in phage-infected bacteria and animal cells. Eur J Biochem. 1974 Feb 15;42(1):157–165. doi: 10.1111/j.1432-1033.1974.tb03325.x. [DOI] [PubMed] [Google Scholar]
  13. Markham P. D., Baluda M. A. Integrated state of oncornavirus DNA in normal chicken cells and in cells transformed by avian myeloblastosis virus. J Virol. 1973 Oct;12(4):721–732. doi: 10.1128/jvi.12.4.721-732.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Obukh I. B., Kryukova I. N. Malignant and transforming activity of Rous sarcoma virus. II. The study of variants of Rous sarcoma virus isolated from mouse tumours. Int J Cancer. 1969 Nov 15;4(6):809–812. doi: 10.1002/ijc.2910040609. [DOI] [PubMed] [Google Scholar]
  15. Ross J., Aviv H., Scolnick E., Leder P. In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer). Proc Natl Acad Sci U S A. 1972 Jan;69(1):264–268. doi: 10.1073/pnas.69.1.264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Scolnick E. M., Parks W. P. Harvey sarcoma virus: a second murine type C sarcoma virus with rat genetic information. J Virol. 1974 Jun;13(6):1211–1219. doi: 10.1128/jvi.13.6.1211-1219.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Scolnick E. M., Rands E., Williams D., Parks W. P. Studies on the nucleic acid sequences of Kirsten sarcoma virus: a model for formation of a mammalian RNA-containing sarcoma virus. J Virol. 1973 Sep;12(3):458–463. doi: 10.1128/jvi.12.3.458-463.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shoyab M., Baluda M. A., Evans R. Acquisition of new DNA sequences after infection of chicken cells with avian myeloblastosis virus. J Virol. 1974 Feb;13(2):331–339. doi: 10.1128/jvi.13.2.331-339.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shoyab M., Evans R. M., Baluda M. A. Presence in leukemic cells of avian myeloblastosis virus-specific DNA sequences absent in normal chicken cells. J Virol. 1974 Jul;14(1):47–49. doi: 10.1128/jvi.14.1.47-49.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shoyab M., Markham P. D., Baluda M. A. Reliability of the RNA-DNA filter hybridization for the detection of oncornavirus-specific DNA sequences. J Virol. 1974 Aug;14(2):225–230. doi: 10.1128/jvi.14.2.225-230.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Simkovic D. Characteristics of tumors induced in mammals, especially rodents, by viruses of the avian leukosis sarcoma group. Adv Virus Res. 1972;17:95–127. [PubMed] [Google Scholar]
  22. THURZO V., SLABEYCIUSOVA M., KLIMEK M., KOVAROVA V. Novaia fil'triruiushchasia kurinaia opukhol'. Cesk Onkol. 1954;1(3-4):230–234. [PubMed] [Google Scholar]
  23. Temin H. M. Mechanism of cell transformation by RNA tumor viruses. Annu Rev Microbiol. 1971;25:609–648. doi: 10.1146/annurev.mi.25.100171.003141. [DOI] [PubMed] [Google Scholar]
  24. Varmus H. E., Vogt P. K., Bishop J. M. Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3067–3071. doi: 10.1073/pnas.70.11.3067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Verma I. M., Temple G. F., Fan H., Baltimore D. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA. Nat New Biol. 1972 Feb 9;235(58):163–167. doi: 10.1038/newbio235163a0. [DOI] [PubMed] [Google Scholar]
  26. Wilson D. A., Thomas C. A., Jr Hydroxyapatite chromatography of short double-helical DNA. Biochim Biophys Acta. 1973 Dec 21;331(3):333–340. doi: 10.1016/0005-2787(73)90019-1. [DOI] [PubMed] [Google Scholar]

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