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. 1975 Mar;72(3):1117–1121. doi: 10.1073/pnas.72.3.1117

Localization of RNA from heat-induced polysomes at puff sites in Drosophila melanogaster.

S L McKenzie, S Henikoff, M Meselson
PMCID: PMC432477  PMID: 805422

Abstract

Heat treatment of D. melanogaster tissue culture cells causes drastic changes in the pattern of protein synthesis and the size distribution of polysomes. Like the heat shock puffs on polytene chromosomes which appear while preexisting puffs regress, heat shock proteins appear on gels while the synthesis of preexisting proteins is sharply reduced, and heat-induced polysomes appear on gradients after preexisting polysomes have disappeared. Most of the poly(adenylic acid)-containing RNA isolated from high-temperature polysomes sediments in sucrose gradients and migrates in gels as a rather narrow band. This RNA is of sufficient size to code for one particular protein that is found to account for more than half of the total synthesis at high temperature. The RNA hybridizes in situ mainly at chromosome sub-division 87B, the site of the major heat shock puff.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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