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. 1975 Apr;72(4):1344–1348. doi: 10.1073/pnas.72.4.1344

Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments.

J B Lewis, J F Atkins, C W Anderson, P R Baum, R F Gesteland
PMCID: PMC432530  PMID: 1093178

Abstract

Cytoplasmic RNA, isolated from cells late after infection by adenovirus type 2 and fractionated by hybridization to specific fragments of adenovirus DNA produced by cleavage with the endonuclease R-EcoRI, was used as template for protein synthesis in cell-free mammalian extracts. Each of the R-EcoRI fragments of DNA selects RNA that encodes specific subsets of the viral polypeptides. From the known order of the R-EcoRI fragments, the following partial map is deduced: (III, IIIa, IVa2, V, P-VII, IX), (II, P-VI), 100K, IV-where the relative order of the components enclosed in parentheses has not yet been determined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson C. W., Baum P. R., Gesteland R. F. Processing of adenovirus 2-induced proteins. J Virol. 1973 Aug;12(2):241–252. doi: 10.1128/jvi.12.2.241-252.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson C. W., Lewis J. B., Atkins J. F., Gesteland R. F. Cell-free synthesis of adenovirus 2 proteins programmed by fractionated messenger RNA: a comparison of polypeptide products and messenger RNA lengths. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2756–2760. doi: 10.1073/pnas.71.7.2756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Craig E. A., Tal J., Nishimoto T., Zimmer S., McGrogan M., Raskas H. J. RNA transcription in cultures productively infected with adenovirus 2. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):483–493. doi: 10.1101/sqb.1974.039.01.060. [DOI] [PubMed] [Google Scholar]
  4. Eron L., Callahan R., Westphal H. Cell-free synthesis of adenovirus coat proteins. J Biol Chem. 1974 Oct 10;249(19):6331–6338. [PubMed] [Google Scholar]
  5. Eron L., Wesphal H., Callahan R. In vitro synthesis of adenovirus core proteins. J Virol. 1974 Aug;14(2):375–383. doi: 10.1128/jvi.14.2.375-383.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eron L., Westphal H. Cell-free translation of highly purified adenovirus messenger RNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3385–3389. doi: 10.1073/pnas.71.9.3385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fuke M., Thomas C. A., Jr Isolation of open-circular DNA molecules by retention in agar gels. J Mol Biol. 1970 Sep 14;52(2):395–397. doi: 10.1016/0022-2836(70)90040-9. [DOI] [PubMed] [Google Scholar]
  8. Goldberg R. B., Galau G. A., Britten R. J., Davidson E. H. Nonrepetitive DNA sequence representation in sea urchin embryo messenger RNA. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3516–3520. doi: 10.1073/pnas.70.12.3516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Grodzicker T., Williams J., Sharp P., Sambrook J. Physical mapping of temperature-sensitive mutations of adenoviruses. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):439–446. doi: 10.1101/sqb.1974.039.01.056. [DOI] [PubMed] [Google Scholar]
  10. Lewis J. B., Anderson C. W., Atkins J. F., Gesteland R. F. The origin and destiny of adenovirus proteins. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):581–590. doi: 10.1101/sqb.1974.039.01.072. [DOI] [PubMed] [Google Scholar]
  11. Mulder C., Arrand J. R., Delius H., Keller W., Pettersson U., Roberts R. J., Sharp P. A. Cleavage maps of DNA from adenovirus types 2 and 5 by restriction endonucleases EcoRI and HpaI. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):397–400. doi: 10.1101/sqb.1974.039.01.051. [DOI] [PubMed] [Google Scholar]
  12. Oberg B., Saborio J., Persson T., Everitt E., Philipson L. Identification of the in vitro translation products of adenovirus mRNA by immunoprecipitation. J Virol. 1975 Jan;15(1):199–207. doi: 10.1128/jvi.15.1.199-207.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pettersson U., Mulder C., Deluis H., Sharp P. A. Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A. 1973 Jan;70(1):200–204. doi: 10.1073/pnas.70.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pettersson U., Sambrook J. Amount of viral DNA in the genome of cells transformed by adenovirus type 2. J Mol Biol. 1973 Jan;73(1):125–130. doi: 10.1016/0022-2836(73)90164-2. [DOI] [PubMed] [Google Scholar]
  15. Philipson L., Pettersson U., Lindberg U., Tibbetts C., Vennström B., Persson T. RNA synthesis and processing in adenovirus-infected cells. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):447–456. doi: 10.1101/sqb.1974.039.01.057. [DOI] [PubMed] [Google Scholar]
  16. Philipson L., Pettersson U. Structure and function of virion proteins of adenoviruses. Prog Exp Tumor Res. 1973;18:1–55. doi: 10.1159/000393160. [DOI] [PubMed] [Google Scholar]
  17. Prives C. L., Aviv H., Paterson B. M., Roberts B. E., Rozenblatt S., Revel M., Winocour E. Cell-free translation of messenger RNA of simian virus 40: synthesis of the major capsid protein. Proc Natl Acad Sci U S A. 1974 Feb;71(2):302–306. doi: 10.1073/pnas.71.2.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sharp P. A., Gallimore P. H., Flint S. J. Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):457–474. doi: 10.1101/sqb.1974.039.01.058. [DOI] [PubMed] [Google Scholar]
  19. Sharp P. A., Pettersson U., Sambrook J. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. J Mol Biol. 1974 Jul 15;86(4):709–726. doi: 10.1016/0022-2836(74)90348-9. [DOI] [PubMed] [Google Scholar]
  20. Tal J., Craig E. A., Zimmer S., Raskas H. J. Localization of adenovirus 2 messenger RNA's to segments of the viral genome defined by endonuclease R-R1. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4057–4061. doi: 10.1073/pnas.71.10.4057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tibbetts C., Pettersson U. Complementary strand-specific sequences from unique fragments of adenovirus type 2 DNA for hybridization-mapping experiments. J Mol Biol. 1974 Oct 5;88(4):767–784. doi: 10.1016/0022-2836(74)90398-2. [DOI] [PubMed] [Google Scholar]
  22. Tibbetts C., Pettersson U., Johansson K., Philpson L. Relationship of mRNA from productively infected cells to the complementary strands of adenovirus type 2 DNA. J Virol. 1974 Feb;13(2):370–377. doi: 10.1128/jvi.13.2.370-377.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Westphal H., Eron L., Ferdinand F. J., Callahan R., Lai S. P. Analysis of adenovirus type 2 gene functions by cell-free translation of viral messenger RNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):575–579. doi: 10.1101/sqb.1974.039.01.071. [DOI] [PubMed] [Google Scholar]
  24. Williams J., Grodzicker T., Sharp P., Sambrook J. Adenovirus recombination: physical mapping of crossover events. Cell. 1975 Feb;4(2):113–119. doi: 10.1016/0092-8674(75)90117-8. [DOI] [PubMed] [Google Scholar]
  25. Willians J. F., Young C. S., Austin P. E. Genetic analysis of human adenovirus type 5 in permissive and nonpermissive cells. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):427–437. doi: 10.1101/sqb.1974.039.01.055. [DOI] [PubMed] [Google Scholar]

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