Fig. 6.

Pros regulates EE cell fate determination in ISCs. (A) The quantitative cell numbers per clone in C-D′. (B) The quantitative percentages of nc82⁺ EEs in GFP+ clones in C-D′. Data are represented as mean±s.e.m. (C-D′) MARCM clones of wild-type control (C,C′) and pros¹⁷ (D,D′). Seven days after clone induction, GFP-marked clones of pros¹⁷ (D,D′) were completely devoid of nc82⁺ EEs (arrow) compared with their wild-type counterparts (C,C′), whereas the clone sizes are similar (A). (E) The numbers of nc82⁺ EE cells in the posterior midguts of pros^RNAi driven by indicated Gal4, compared with wild-type controls. Data are represented as mean±s.e.m. *P<0.01. (F,F′) The overexpression of sc in ISCs and EBs (esg^ts>sc). (G,G′) The overexpression of sc and pros^RNAi in ISCs and EBs (esg^ts>sc+pros^RNAi). The overexpression of pros^RNAi suppressed the excess EE phenotype associated with sc overexpression, indicating that Pros functions either downstream of or parallel to Sc. The adult fly posterior midguts were stained with anti-GFP (green), anti-nc82 (red) and DAPI (blue). Scale bars: 10 μm.