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. 1975 Apr;72(4):1392–1396. doi: 10.1073/pnas.72.4.1392

Biochemical procedure for production of small deletions in simian virus 40 DNA.

J Carbon, T E Shenk, P Berg
PMCID: PMC432540  PMID: 165505

Abstract

A simple biochemical procedure for producing small deletions (15 to 50 base pairs) at virtually any location in simian virus 40 DNA has been developed. The steps involved are: cleavage of the closed-circular DNA to produce a linear structure followed by 5'-exonuclease digestion to expose a short single-stranded segment at each 3' end of the molecule. Mutants containing deletions at the site of the cleavage are obtained by infecting permissive monkey kidney cells with the exonuclease-treated DNA in the presence or absence of a helper DNA (depending upon whether or not the site of cleavage and therefore the deletion occurred in a gene required for vegetative multiplication). In this paper viable mutants with deletions at the HpaII endonuclease cleavage site (0.735 map position) and defective trans-complementable mutants with deletions at the EcoRI endonuclease cleavage site (0/1.0 map position) were isolated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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