Abstract
Bacteriophage T4 serine and proline transfer RNAs are derived from a common precursor RNA. This precusor RNA lacks -C-C-A sequences which could provide 3' termini for the mature transfer RNAs. We have deduced part of the pathway leading to the formation of the C-C-A sequences in the transfer RNAs by characterizing incompletely matured precursor molecules which accumulate during infection of mutant hosts that lack specific enzymes associated with transfer RNA metabolism. Maturation is initiated by the addition of -C-C-AOH to the 3' terminus of the precusor RNA through the combined actionof an unidentified nuclease and tRNA nucleotidyltransferase (EC 2.7.7.25). Precursor RNA molecules terminating in -C-C-AOH is serine transfer RNA and the second product is immature proline transfer RNA. The terminal steps leading to proline transfer RNA have not been fully delineated, but are known to involve the replacement of a -C-UOH sequence by -C-C-AOH.
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- Barrell B. G., Seidman J. G., Guthrie C., McClain W. H. Transfer RNA biosynthesis: the nucleotide sequence of a precursor to serine and proline transfer RNAs. Proc Natl Acad Sci U S A. 1974 Feb;71(2):413–416. doi: 10.1073/pnas.71.2.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Comer M. M., Guthrie C., McClain W. H. An ochre suppressor of bacteriophage T4 that is associated with a transfer RNA. J Mol Biol. 1974 Dec 25;90(4):665–676. doi: 10.1016/0022-2836(74)90531-2. [DOI] [PubMed] [Google Scholar]
- Deutscher M. P., Foulds J., McClain W. H. Transfer ribonucleic acid nucleotidyl-transferase plays an essential role in the normal growth of Escherichia coli and in the biosynthesis of some bacteriophage T4 transfer ribonucleic acids. J Biol Chem. 1974 Oct 25;249(20):6696–6699. [PubMed] [Google Scholar]
- Foulds J., Hilderman R. H., Deutscher M. P. Mapping of the locus for Escherichia coli transfer ribonucleic acid nucleotidyltransferase. J Bacteriol. 1974 May;118(2):628–632. doi: 10.1128/jb.118.2.628-632.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guthrie C., McClain W. H. Conditionally lethal mutants of bacteriophage T4 defective in production of a transfer RNA. J Mol Biol. 1973 Dec 5;81(2):137–155. doi: 10.1016/0022-2836(73)90185-x. [DOI] [PubMed] [Google Scholar]
- Guthrie C., Seidman J. G., Altman S., Barrell B. G., Smith J. D., McClain W. H. Identification of tRNA precursor molecules made by phage T4. Nat New Biol. 1973 Nov 7;246(149):6–11. doi: 10.1038/newbio246006a0. [DOI] [PubMed] [Google Scholar]
- McClain W. H., Guthrie C., Barrell B. G. Eight transfer RNAs induced by infection of Escherichia coli with bacteriophage T4. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3703–3707. doi: 10.1073/pnas.69.12.3703. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McClain W. H., Guthrie C., Barrell B. G. The psu1+ amber suppressor gene of bacteriophage T4: identification of its amino acid and transfer RNA. J Mol Biol. 1973 Dec 5;81(2):157–171. doi: 10.1016/0022-2836(73)90186-1. [DOI] [PubMed] [Google Scholar]
- Robertson H. D., Altman S., Smith J. D. Purification and properties of a specific Escherichia coli ribonuclease which cleaves a tyrosine transfer ribonucleic acid presursor. J Biol Chem. 1972 Aug 25;247(16):5243–5251. [PubMed] [Google Scholar]
- Schedl P., Primakoff P. Mutants of Escherichia coli thermosensitive for the synthesis of transfer RNA. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2091–2095. doi: 10.1073/pnas.70.7.2091. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seidman J. G., Comer M. M., McClain W. H. Nucleotide alterations in the bacteriophage T4 glutamine transfer RNA that affect ochre suppressor activity. J Mol Biol. 1974 Dec 25;90(4):677–689. doi: 10.1016/0022-2836(74)90532-4. [DOI] [PubMed] [Google Scholar]