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. 1975 Apr;72(4):1584–1588. doi: 10.1073/pnas.72.4.1584

Induction of growth in resting fibroblastic cell cultures by Ca++.

R Dulbecco, J Elkington
PMCID: PMC432582  PMID: 165515

Abstract

Of all the components of the culture medium, only CaCl2 induces DNA replication when added to resting cultures of Balb/c 3T3 cells. The effect is present even in a serum-free medium. Increasing the Ca++ concentration above the standard 1.8 mM in the medium of a new culture increases the total number of cells ultimately produced, without affecting the initial cell growth rate. This effect is synergistic with that of serum. The elevated Ca++ concentration also induces striking morphological changes. The Ca++ effect could not be reproduced by a Ca++ ionophore. These observations afford a new tool for studying how the various intracellular events following the addition of growth factors to resting cultures are involved in the control of cellular growth.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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