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. 1994 Mar 1;91(5):1848–1852. doi: 10.1073/pnas.91.5.1848

Structure, function, and chromosome mapping of the growth-suppressing human homologue of the murine gas1 gene.

G Del Sal 1, L Collavin 1, M E Ruaro 1, P Edomi 1, S Saccone 1, G D Valle 1, C Schneider 1
PMCID: PMC43261  PMID: 8127893

Abstract

We describe the isolation, growth-suppressing activity, and chromosomal localization of the human homologue of the murine growth-arrest-specific gene gas1. Overexpression of h-gas1 is able to block cell proliferation in the A549 lung carcinoma and the T24 bladder carcinoma cell lines. No effect was observed when h-gas1 was introduced into the osteosarcoma cell line SAOS-2 and into the adenovirus-type-5 transformed cell line 293. This finding is related to our previous evidence that simian virus 40-transformed NIH 3T3 cells are also refractory to murine gas1 overexpression, suggesting that the retinoblastoma and/or p53 gene products have an active role in mediating the growth-suppressing effect of gas1. We also show that h-gas1 is on chromosome 9q21.3-22.1, in a region considered to be a fragile site. Altogether, the results raise the possibility that h-gas1 may be a target for genetic alterations leading to its inactivation in tumor cells.

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