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. 1975 May;72(5):1873–1877. doi: 10.1073/pnas.72.5.1873

Somatic hybrid of thymus leukemia (.

W Liang, E P Cohen
PMCID: PMC432649  PMID: 168580

Abstract

A somatic hybrid of ASL-1 leukemia cells [H-2a, thymus leukemia (TL)1,2,3, Thy-1b] and LM(TK)-cells [H-2-k, TL(-), Thy-1 (-), thymidine kinase deficient] was formed with the aid of inactivated Sendai virus. The selection of hybrid cell clones was facilitated by the inability of ASL-1 cells to grow in vitro and of LM(TK)-cells to survive in hypoxanthine/amethopterin/thymidine medium. The H-2 antigens of both parental cells were formed in approximately equivalent amounts by the hybrid cells, and they possessed a hybrid karyotype. As determined by five independent experimental procedures (antibody and complement-mediated cytotoxicity tests, the reduction of specific antibody activity of antiserum of known titer, immunofluorescent tests, mixed hemagglutination tests, and their direct isolation), TL antigens but not Thy-1 antigens were formed by the hybrid cells. TL antigens of the hybrids failed to undergo modulation under conditions leading to the modulation of TL antigens of parental ASL-1 cells. Modulation was attempted with TL 1,3, TL 2, or TL 1,2,3 antisera of high titer. thybrid cells were incubated for up to 30 hr in medium with TL antisera. Both direct and indirect immune methods were attempted. These results indicate that cellular mechanisms controlling the expression of TL antigens may be distinguished from the capacity of the cells to undergo modulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoki T., Johnson P. A. Suppression of Gross leukemia cell-surface antigens: a kind of antigenic modulation. J Natl Cancer Inst. 1972 Jul;49(1):183–192. [PubMed] [Google Scholar]
  2. Boyse E. A., Stockert E., Iritani C. A., Old L. J. Implications of TL phenotype changes in an H-2-loss variant of a transplanted H-2b-H-2a leukemia. Proc Natl Acad Sci U S A. 1970 Apr;65(4):933–938. doi: 10.1073/pnas.65.4.933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Croce C. M., Koprowski H., Eagle H. Effect of environmental pH on the efficiency of cellular hybridization. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1953–1956. doi: 10.1073/pnas.69.7.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DUBBS D. R., KIT S. EFFECT OF HALOGENATED PYRIMIDINES AND THYMIDINE ON GROWTH OF L-CELLS AND A SUBLINE LACKING THYMIDINE KINASE. Exp Cell Res. 1964 Jan;33:19–28. doi: 10.1016/s0014-4827(64)81006-5. [DOI] [PubMed] [Google Scholar]
  5. Frye L. D., Edidin M. The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons. J Cell Sci. 1970 Sep;7(2):319–335. doi: 10.1242/jcs.7.2.319. [DOI] [PubMed] [Google Scholar]
  6. Giles R. E., Ruddle F. H. Production of Sendai virus for cell fusion. In Vitro. 1973 Sep-Oct;9(2):103–107. doi: 10.1007/BF02616007. [DOI] [PubMed] [Google Scholar]
  7. KIT S., DUBBS D. R., PIEKARSKI L. J., HSU T. C. DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE. Exp Cell Res. 1963 Aug;31:297–312. doi: 10.1016/0014-4827(63)90007-7. [DOI] [PubMed] [Google Scholar]
  8. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  9. MILGROM F., KANO K., BARRON A. L., WITEBSKY E. MIXED AGGLUTINATION IN TISSUE CULTURE. J Immunol. 1964 Jan;92:8–16. [PubMed] [Google Scholar]
  10. Old L. J., Stockert E., Boyse E. A., Kim J. H. Antigenic modulation. Loss of TL antigen from cells exposed to TL antibody. Study of the phenomenon in vitro. J Exp Med. 1968 Mar 1;127(3):523–539. doi: 10.1084/jem.127.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. ROTHFELS K. H., SIMINOVITCH L. An air-drying technique for flattening chromosomes in mammalian oells grown in vitro. Stain Technol. 1958 Mar;33(2):73–77. doi: 10.3109/10520295809111827. [DOI] [PubMed] [Google Scholar]
  12. SPENCER R. A., HAUSCHKA T. S., AMOS D. B., EPHRUSSI B. CO-DOMINANCE OF ISOANTIGENS IN SOMATIC HYBRIDS OF MURINE CELLS GROWN IN VITRO. J Natl Cancer Inst. 1964 Nov;33:893–903. doi: 10.1093/jnci/33.5.893. [DOI] [PubMed] [Google Scholar]
  13. Schwartz B. D., Nathenson S. G. Isolation of H-2 alloantigens solubilized by the detergent NP-40. J Immunol. 1971 Nov;107(5):1363–1367. [PubMed] [Google Scholar]
  14. Yu A., Cohen E. P. Studies on the effect of specific antisera on the metabolism of cellular antigens. I. Isolation of thymus leukemia antigens. J Immunol. 1974 Apr;112(4):1285–1295. [PubMed] [Google Scholar]
  15. Yu A., Cohen E. P. Studies on the effect of specific antisera on the metabolism of cellular antigens. II. The synthesis and degradation of TL antigens of mouse cells in the presence of TL antiserum. J Immunol. 1974 Apr;112(4):1296–1307. [PubMed] [Google Scholar]

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