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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 May;72(5):1932–1936. doi: 10.1073/pnas.72.5.1932

In vitro transformation of lymphoid cells by Abelson murine leukemia virus.

N Rosenberg, D Baltimore, C D Scher
PMCID: PMC432662  PMID: 168584

Abstract

Cell cultures prepared from fetal murine liver were infected by Abelson murine leukemia virus. After about 2 weeks, proliferating cells of lymphoid morphology appeared in some of the cultures. Addition of 2-mercaptoethanol to the initial culture medium greatly enhanced the appearance of the lymphoid cells. Immunoglobulin determinants were evident on the cells in some cultures. Continuous passage of the cells in certain cultures was possible and the passaged cells could form tumors after animal inoculation. Because Abelson murine leukemia virus is able to induce in vitro malignant transformation of lymphoid cells, it probably causes leukemia by directly affecting cellular growth control.

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Selected References

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  1. Abelson H. T., Rabstein L. S. Lymphosarcoma: virus-induced thymic-independent disease in mice. Cancer Res. 1970 Aug;30(8):2213–2222. [PubMed] [Google Scholar]
  2. BALUDA M. A., GOETZ I. E. Morphological conversion of cell cultures by avian myeloblastosis virus. Virology. 1961 Oct;15:185–199. doi: 10.1016/0042-6822(61)90234-3. [DOI] [PubMed] [Google Scholar]
  3. BALUDA M. A. Properties of cells infected with avian myeloblastosis virus. Cold Spring Harb Symp Quant Biol. 1962;27:415–425. doi: 10.1101/sqb.1962.027.001.039. [DOI] [PubMed] [Google Scholar]
  4. Broome J. D., Jeng M. W. Growth stimulation of mouse leukemia cells by thiols and disulfides in vitro. J Natl Cancer Inst. 1972 Aug;49(2):579–581. [PubMed] [Google Scholar]
  5. Broome J. D., Jeng M. W. Promotion of replication in lymphoid cells by specific thiols and disulfides in vitro. Effects on mouse lymphoma cells in comparison with splenic lymphocytes. J Exp Med. 1973 Sep 1;138(3):574–592. doi: 10.1084/jem.138.3.574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chen C., Hirsch J. G. Restoration of antibody-forming capacity in cultures of nonadherent spleen cells by mercaptoethanol. Science. 1972 Apr 7;176(4030):60–61. doi: 10.1126/science.176.4030.60. [DOI] [PubMed] [Google Scholar]
  7. Fan H., Paskind M. Measurement of the sequence complexity of cloned Moloney murine leukemia virus 60 to 70S RNA: evidence for a haploid genome. J Virol. 1974 Sep;14(3):421–429. doi: 10.1128/jvi.14.3.421-429.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Geha R. S., Schneeberger E., Rosen F. S., Merler E. Interaction of human thymus-derived and non-thymus-derived lymphocytes in vitro. Induction of proliferation and antibody synthesis in B lymphocytes by a soluble factor released from antigen-stimulated T lymphocytes. J Exp Med. 1973 Nov 1;138(5):1230–1247. doi: 10.1084/jem.138.5.1230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miller G., Shope T., Lisco H., Stitt D., Lipman M. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc Natl Acad Sci U S A. 1972 Feb;69(2):383–387. doi: 10.1073/pnas.69.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moscovici C., Vogt P. K. Effects of genetic cellular resistance on cell transformation and virus replication in chicken hematopoietic cell cultures infected with avian myeloblastosis virus (BAI-A). Virology. 1968 Aug;35(4):487–497. doi: 10.1016/0042-6822(68)90278-x. [DOI] [PubMed] [Google Scholar]
  11. Moscovici C., Zanetti M. Studies on single foci of hematopoietic cells transformed by avian myeloblastosis virus. Virology. 1970 Sep;42(1):61–67. doi: 10.1016/0042-6822(70)90238-2. [DOI] [PubMed] [Google Scholar]
  12. Nossal G. J., Pike B. L. Differentiation of B lymphocytes from stem cell precursors. Adv Exp Med Biol. 1973;29(0):11–18. doi: 10.1007/978-1-4615-9017-0_3. [DOI] [PubMed] [Google Scholar]
  13. Parkman R. Tumor hybrid cells: an immunotherapeutic agent. J Natl Cancer Inst. 1974 May;52(5):1541–1545. doi: 10.1093/jnci/52.5.1541. [DOI] [PubMed] [Google Scholar]
  14. Potter M., Sklar M. D., Rowe W. P. Rapid viral induction of plasmacytomas in pristane-primed BALB-c mice. Science. 1973 Nov 9;182(4112):592–594. doi: 10.1126/science.182.4112.592. [DOI] [PubMed] [Google Scholar]
  15. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  16. Scher C. D., Siegler R. Direct transformation of 3T3 cells by Abelson murine leukaemia virus. Nature. 1975 Feb 27;253(5494):729–731. doi: 10.1038/253729a0. [DOI] [PubMed] [Google Scholar]
  17. Shevach E. M., Stobo J. D., Green I. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J Immunol. 1972 May;108(5):1146–1151. [PubMed] [Google Scholar]
  18. Shope T., Dechairo D., Miller G. Malignant lymphoma in cottontop marmosets after inoculation with Epstein-Barr virus. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2487–2491. doi: 10.1073/pnas.70.9.2487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Siegler R., Rich M. A. Pathogenesis of murine leukemia. Natl Cancer Inst Monogr. 1966 Sep;22:525–547. [PubMed] [Google Scholar]
  20. Siegler R., Zajdel S. Pathogenesis of Abelson-virus-induced murine leukemia. J Natl Cancer Inst. 1972 Jan;48(1):189–218. [PubMed] [Google Scholar]
  21. Sklar M. D., Shevach E. M., Green I., Potter M. Transplantation and preliminary characterisation of lymphocyte surface markers of Abelson virus-induced lymphomas. Nature. 1975 Feb 13;253(5492):550–552. doi: 10.1038/253550a0. [DOI] [PubMed] [Google Scholar]
  22. Sklar M. D., White B. J., Rowe W. P. Initiation of oncogenic transformation of mouse lymphocytes in vitro by Abelson leukemia virus. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4077–4081. doi: 10.1073/pnas.71.10.4077. [DOI] [PMC free article] [PubMed] [Google Scholar]

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