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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 May;72(5):1960–1964. doi: 10.1073/pnas.72.5.1960

Interaction of partially purified simian virus 40 T antigen with circular viral DNA molecules.

D Jessel, J Hudson, T Landau, D Tenen, D M Livingston
PMCID: PMC432668  PMID: 168585

Abstract

Mixing chromatographic fractions containing simian virus 40 (SV40) T antigen with SV40 [3H]-DNA I (double-stranded, circular, supercoiled) results in the conversion of the nucleic acid to a form that will bind to a nitrocellulose filter. Unlabeled SV40 DNA I successfully competes with this reaction. Under the conditions employed, the antigen-containing fractions bind a variety of circular, viral DNA molecules. Chromatography of the antigen in three systems reveals that the T immunoreactivity migrates with DNA binding activity. In a kinetic heat inactivation experiment, the antigenic reactivity disappears simultaneously with the DNA binding activity. The data indicate the presence of a discernible DNA binding activity in fractions containing T antigen and suggest that the T antigen is the DNA binding protein being measured.

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1960

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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