Abstract
NAD is a necessary cofactor for the activation of adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) by cholera toxin. Lysates of certain types of cell that hydrolyze their endogenous store of NAD after cell disruption respond poorly or not at all to cholera toxin. Lysates of pigeon erythrocytes, which lack enzymes that degrade NAD, provide a convenient and reproducible system for assaying the activity of cholera toxin in vitro and allow investigation of the mechanism of action of the toxin upon broken cells.
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Selected References
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- Gill D. M. Poly (adenosine diphosphate ribose) synthesis in soluble extracts of animal organs. J Biol Chem. 1972 Sep 25;247(18):5964–5971. [PubMed] [Google Scholar]
- Goor R. S., Pappenheimer A. M., Jr Studies on the mode of action of diphtheria toxin. IV. Specificity of the cofactor (NAD) requirement for toxin action in cell-free systems. J Exp Med. 1967 Nov 1;126(5):913–921. doi: 10.1084/jem.126.5.913. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOFMANN E. C., RAPOPORT S. DPN- und TPN-spezifische Nukleosidasen in Erythrozyten. Biochim Biophys Acta. 1955 Oct;18(2):296–296. doi: 10.1016/0006-3002(55)90076-7. [DOI] [PubMed] [Google Scholar]
- Stanley P. E. Determination of subpicomole levels of NADH and FMN using bacterial luciferase and the liquid scintillation spectrometer. Anal Biochem. 1971 Feb;39(2):441–453. doi: 10.1016/0003-2697(71)90434-9. [DOI] [PubMed] [Google Scholar]