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. 1975 Jun;72(6):2242–2246. doi: 10.1073/pnas.72.6.2242

Cloning, isolation, and characterization of replication regions of complex plasmid genomes.

K Timmis, F Cabello, S N Cohen
PMCID: PMC432733  PMID: 1094460

Abstract

EcoRI endonuclease-generated DNA fragments carrying replication regions of the F'lac and R6-5 plasmids have been cloned and isolated, using as a selection vehicle a nonreplicating ampicillin-resistance DNA fragment derived from a Staphylococcus aureus plasmid. Heteroduplex analysis of the constructed plasmid chimeras and the parent replicons has localized the cloned R6-5 replication region to a DNA segment between kilobase pair coordinates 1.0 and 88.0 on the R6-5 map. Physical proximity between the plasmid replication functions and the locus governing plasmid incompatibility has been shown for both parent replicons. The cloning method reported appears to be generally applicable for the identification and isolation of replication regions of a variety of complex genomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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