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. 1994 Mar 1;91(5):1913–1916. doi: 10.1073/pnas.91.5.1913

Nuclear matrix proteins in human colon cancer.

S K Keesee 1, M D Meneghini 1, R P Szaro 1, Y J Wu 1
PMCID: PMC43274  PMID: 8127905

Abstract

The nuclear matrix is the nonchromatin scaffolding of the nucleus. This structure confers nuclear shape, organizes chromatin, and appears to contain important regulatory proteins. Tissue specific nuclear matrix proteins have been found in the rat, mouse, and human. In this study we compared high-resolution two-dimensional gel electropherograms of nuclear matrix protein patterns found in human colon tumors with those from normal colon epithelia. Tumors were obtained from 18 patients undergoing partial colectomy for adenocarcinoma of the colon and compared with tissue from 10 normal colons. We have identified at least six proteins which were present in 18 of 18 colon tumors and 0 of 10 normal tissues, as well as four proteins present in 0 of 18 tumors and in 10 of 10 normal tissues. These data, which corroborate similar findings of cancer-specific nuclear matrix proteins in prostate and breast, suggest that nuclear matrix proteins may serve as important markers for at least some types of cancer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aebi U., Cohn J., Buhle L., Gerace L. The nuclear lamina is a meshwork of intermediate-type filaments. Nature. 1986 Oct 9;323(6088):560–564. doi: 10.1038/323560a0. [DOI] [PubMed] [Google Scholar]
  2. Baker S. J., Fearon E. R., Nigro J. M., Hamilton S. R., Preisinger A. C., Jessup J. M., vanTuinen P., Ledbetter D. H., Barker D. F., Nakamura Y. Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas. Science. 1989 Apr 14;244(4901):217–221. doi: 10.1126/science.2649981. [DOI] [PubMed] [Google Scholar]
  3. Berezney R., Coffey D. S. Identification of a nuclear protein matrix. Biochem Biophys Res Commun. 1974 Oct 23;60(4):1410–1417. doi: 10.1016/0006-291x(74)90355-6. [DOI] [PubMed] [Google Scholar]
  4. Bidwell J. P., Van Wijnen A. J., Fey E. G., Dworetzky S., Penman S., Stein J. L., Lian J. B., Stein G. S. Osteocalcin gene promoter-binding factors are tissue-specific nuclear matrix components. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3162–3166. doi: 10.1073/pnas.90.8.3162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bos J. L., Fearon E. R., Hamilton S. R., Verlaan-de Vries M., van Boom J. H., van der Eb A. J., Vogelstein B. Prevalence of ras gene mutations in human colorectal cancers. 1987 May 28-Jun 3Nature. 327(6120):293–297. doi: 10.1038/327293a0. [DOI] [PubMed] [Google Scholar]
  6. Brancolini C., Schneider C. Change in the expression of a nuclear matrix-associated protein is correlated with cellular transformation. Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):6936–6940. doi: 10.1073/pnas.88.16.6936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Compton D. A., Szilak I., Cleveland D. W. Primary structure of NuMA, an intranuclear protein that defines a novel pathway for segregation of proteins at mitosis. J Cell Biol. 1992 Mar;116(6):1395–1408. doi: 10.1083/jcb.116.6.1395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dworetzky S. I., Wright K. L., Fey E. G., Penman S., Lian J. B., Stein J. L., Stein G. S. Sequence-specific DNA-binding proteins are components of a nuclear matrix-attachment site. Proc Natl Acad Sci U S A. 1992 May 1;89(9):4178–4182. doi: 10.1073/pnas.89.9.4178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Earnshaw W. C., Heck M. M. Localization of topoisomerase II in mitotic chromosomes. J Cell Biol. 1985 May;100(5):1716–1725. doi: 10.1083/jcb.100.5.1716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fearon E. R., Vogelstein B. A genetic model for colorectal tumorigenesis. Cell. 1990 Jun 1;61(5):759–767. doi: 10.1016/0092-8674(90)90186-i. [DOI] [PubMed] [Google Scholar]
  11. Fey E. G., Krochmalnic G., Penman S. The nonchromatin substructures of the nucleus: the ribonucleoprotein (RNP)-containing and RNP-depleted matrices analyzed by sequential fractionation and resinless section electron microscopy. J Cell Biol. 1986 May;102(5):1654–1665. doi: 10.1083/jcb.102.5.1654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fey E. G., Penman S. Nuclear matrix proteins reflect cell type of origin in cultured human cells. Proc Natl Acad Sci U S A. 1988 Jan;85(1):121–125. doi: 10.1073/pnas.85.1.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Fey E. G., Wan K. M., Penman S. Epithelial cytoskeletal framework and nuclear matrix-intermediate filament scaffold: three-dimensional organization and protein composition. J Cell Biol. 1984 Jun;98(6):1973–1984. doi: 10.1083/jcb.98.6.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gerace L., Blum A., Blobel G. Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution. J Cell Biol. 1978 Nov;79(2 Pt 1):546–566. doi: 10.1083/jcb.79.2.546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gerace L., Burke B. Functional organization of the nuclear envelope. Annu Rev Cell Biol. 1988;4:335–374. doi: 10.1146/annurev.cb.04.110188.002003. [DOI] [PubMed] [Google Scholar]
  16. Getzenberg R. H., Coffey D. S. Tissue specificity of the hormonal response in sex accessory tissues is associated with nuclear matrix protein patterns. Mol Endocrinol. 1990 Sep;4(9):1336–1342. doi: 10.1210/mend-4-9-1336. [DOI] [PubMed] [Google Scholar]
  17. Getzenberg R. H., Pienta K. J., Huang E. Y., Coffey D. S. Identification of nuclear matrix proteins in the cancer and normal rat prostate. Cancer Res. 1991 Dec 15;51(24):6514–6520. [PubMed] [Google Scholar]
  18. Greenfield I., Nickerson J., Penman S., Stanley M. Human papillomavirus 16 E7 protein is associated with the nuclear matrix. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11217–11221. doi: 10.1073/pnas.88.24.11217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Gryska P. V., Cohen A. M. Screening asymptomatic patients at high risk for colon cancer with full colonoscopy. Dis Colon Rectum. 1987 Jan;30(1):18–20. doi: 10.1007/BF02556913. [DOI] [PubMed] [Google Scholar]
  20. He D. C., Nickerson J. A., Penman S. Core filaments of the nuclear matrix. J Cell Biol. 1990 Mar;110(3):569–580. doi: 10.1083/jcb.110.3.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kaufmann S. H., Gibson W., Shaper J. H. Characterization of the major polypeptides of the rat liver nuclear envelope. J Biol Chem. 1983 Feb 25;258(4):2710–2719. [PubMed] [Google Scholar]
  22. Khanuja P. S., Lehr J. E., Soule H. D., Gehani S. K., Noto A. C., Choudhury S., Chen R., Pienta K. J. Nuclear matrix proteins in normal and breast cancer cells. Cancer Res. 1993 Jul 15;53(14):3394–3398. [PubMed] [Google Scholar]
  23. Moertel C. G., Fleming T. R., Macdonald J. S., Haller D. G., Laurie J. A., Tangen C. An evaluation of the carcinoembryonic antigen (CEA) test for monitoring patients with resected colon cancer. JAMA. 1993 Aug 25;270(8):943–947. [PubMed] [Google Scholar]
  24. Mulder J. W., Offerhaus G. J., de Feyter E. P., Floyd J. J., Kern S. E., Vogelstein B., Hamilton S. R. The relationship of quantitative nuclear morphology to molecular genetic alterations in the adenoma-carcinoma sequence of the large bowel. Am J Pathol. 1992 Oct;141(4):797–804. [PMC free article] [PubMed] [Google Scholar]
  25. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  26. Oakley B. R., Kirsch D. R., Morris N. R. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980 Jul 1;105(2):361–363. doi: 10.1016/0003-2697(80)90470-4. [DOI] [PubMed] [Google Scholar]
  27. Ogata N. Preferential association of a transcriptionally active gene with the nuclear matrix of rat fibroblasts transformed by a simian-virus-40-pBR322 recombinant plasmid. Biochem J. 1990 Apr 15;267(2):385–390. doi: 10.1042/bj2670385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Olson A. D., Miller M. J. Elsie 4: quantitative computer analysis of sets of two-dimensional gel electrophoretograms. Anal Biochem. 1988 Feb 15;169(1):49–70. doi: 10.1016/0003-2697(88)90255-2. [DOI] [PubMed] [Google Scholar]
  29. Partin A. W., Getzenberg R. H., CarMichael M. J., Vindivich D., Yoo J., Epstein J. I., Coffey D. S. Nuclear matrix protein patterns in human benign prostatic hyperplasia and prostate cancer. Cancer Res. 1993 Feb 15;53(4):744–746. [PubMed] [Google Scholar]
  30. Pienta K. J., Coffey D. S. A structural analysis of the role of the nuclear matrix and DNA loops in the organization of the nucleus and chromosome. J Cell Sci Suppl. 1984;1:123–135. doi: 10.1242/jcs.1984.supplement_1.9. [DOI] [PubMed] [Google Scholar]
  31. Stuurman N., Meijne A. M., van der Pol A. J., de Jong L., van Driel R., van Renswoude J. The nuclear matrix from cells of different origin. Evidence for a common set of matrix proteins. J Biol Chem. 1990 Apr 5;265(10):5460–5465. [PubMed] [Google Scholar]
  32. Vogelstein B., Fearon E. R., Hamilton S. R., Kern S. E., Preisinger A. C., Leppert M., Nakamura Y., White R., Smits A. M., Bos J. L. Genetic alterations during colorectal-tumor development. N Engl J Med. 1988 Sep 1;319(9):525–532. doi: 10.1056/NEJM198809013190901. [DOI] [PubMed] [Google Scholar]
  33. Winawer S. J., Schottenfeld D., Flehinger B. J. Colorectal cancer screening. J Natl Cancer Inst. 1991 Feb 20;83(4):243–253. doi: 10.1093/jnci/83.4.243. [DOI] [PubMed] [Google Scholar]
  34. Zeitlin S., Parent A., Silverstein S., Efstratiadis A. Pre-mRNA splicing and the nuclear matrix. Mol Cell Biol. 1987 Jan;7(1):111–120. doi: 10.1128/mcb.7.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Zeitlin S., Wilson R. C., Efstratiadis A. Autonomous splicing and complementation of in vivo-assembled spliceosomes. J Cell Biol. 1989 Mar;108(3):765–777. doi: 10.1083/jcb.108.3.765. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. von Kries J. P., Buhrmester H., Strätling W. H. A matrix/scaffold attachment region binding protein: identification, purification, and mode of binding. Cell. 1991 Jan 11;64(1):123–135. doi: 10.1016/0092-8674(91)90214-j. [DOI] [PubMed] [Google Scholar]

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