Abstract
Poly(adenylic acid) sequences have been isolated from the Gram-negative bacterium Caulobacter crescentus. Most of these A-rich tracts are associated with large RNA molecules that constitute an important fraction of the unstable RNA in these bacteria, and, as estimated by poly(U) filter binding, they are not present in the 16S or 23S ribosomal RNA. Preliminary estimates of size from polyacrylamide gel electrophoresis suggest that the majority of the A-rich tracts ranges from 15 to approximately 50 residues in length.
Full text
PDF![2343](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bca4/432754/8d83cf7d4e4a/pnas00049-0369.png)
![2344](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bca4/432754/b112cdcf65af/pnas00049-0370.png)
![2345](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bca4/432754/f558eb7c8981/pnas00049-0371.png)
![2346](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bca4/432754/8de856b22aa8/pnas00049-0372.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Darnell J. E., Jelinek W. R., Molloy G. R. Biogenesis of mRNA: genetic regulation in mammalian cells. Science. 1973 Sep 28;181(4106):1215–1221. doi: 10.1126/science.181.4106.1215. [DOI] [PubMed] [Google Scholar]
- Firtel R. A., Jacobson A., Lodish H. F. Isolation and hybridization kinetics of messenger RNA from Dictyostelium discoideum. Nat New Biol. 1972 Oct 25;239(95):225–228. doi: 10.1038/newbio239225a0. [DOI] [PubMed] [Google Scholar]
- Jacobson A., Firtel R. A., Lodish H. F. Synthesis of messenger and ribosomal RNA precursors in isolated nuclei of the cellular slime mold Dictyostelium discoideum. J Mol Biol. 1974 Jan 15;82(2):213–230. doi: 10.1016/0022-2836(74)90342-8. [DOI] [PubMed] [Google Scholar]
- Jacobson A., Firtel R. A., Lodish H. Transcription of polydeoxythymidylate sequences in the genome of the cellular slime mold, Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1974 May;71(5):1607–1611. doi: 10.1073/pnas.71.5.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kates J. Detection and utilization of poly(A) sequences in messenger RNA. Methods Cell Biol. 1973;7:53–65. doi: 10.1016/s0091-679x(08)61771-9. [DOI] [PubMed] [Google Scholar]
- McLaughlin C. S., Warner J. R., Edmonds M., Nakazato H., Vaughan M. H. Polyadenylic acid sequences in yeast messenger ribonucleic acid. J Biol Chem. 1973 Feb 25;248(4):1466–1471. [PubMed] [Google Scholar]
- Newton A. Role of transcription in the temporal control of development in Caulobacter crescentus (stalk-rifampin-RNA synthesis-DNA synthesis-motility). Proc Natl Acad Sci U S A. 1972 Feb;69(2):447–451. doi: 10.1073/pnas.69.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Niyogi S. K. The influence of chain length and base composition on the specific association of oligoribonucleotides with denatured deoxyribonucleic acid. J Biol Chem. 1969 Mar 25;244(6):1576–1581. [PubMed] [Google Scholar]
- POINDEXTER J. S. BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP. Bacteriol Rev. 1964 Sep;28:231–295. doi: 10.1128/br.28.3.231-295.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perlman S., Abelson H. T., Penman S. Mitochondrial protein synthesis: RNA with the properties of Eukaryotic messenger RNA. Proc Natl Acad Sci U S A. 1973 Feb;70(2):350–353. doi: 10.1073/pnas.70.2.350. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perry R. P., Kelley D. E., LaTorre J. Lack of polyadenylic acid sequences in the messenger RNA of E. coli. Biochem Biophys Res Commun. 1972 Sep 26;48(6):1593–1600. doi: 10.1016/0006-291x(72)90896-0. [DOI] [PubMed] [Google Scholar]
- Schmidt J. M., Stanier R. Y. The development of cellular stalks in bacteria. J Cell Biol. 1966 Mar;28(3):423–436. doi: 10.1083/jcb.28.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sheldon R., Jurale C., Kates J. Detection of polyadenylic acid sequences in viral and eukaryotic RNA(polu(U)-cellulose columns-poly(U) filters-fiberglass-HeLa cells-bacteriophage T4). Proc Natl Acad Sci U S A. 1972 Feb;69(2):417–421. doi: 10.1073/pnas.69.2.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van de Walle C. Polyadenylic sequences in plant RNA. FEBS Lett. 1973 Aug 1;34(1):31–34. doi: 10.1016/0014-5793(73)80696-9. [DOI] [PubMed] [Google Scholar]