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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Jun;72(6):2375–2379. doi: 10.1073/pnas.72.6.2375

Temperature-sensitive RNA polymerase mutants with altered subunit synthesis and degradation.

J B Kirschbaum, I V Claeys, S Nasi, B Molholt, J H Miller
PMCID: PMC432761  PMID: 1094466

Abstract

A temperature-sensitive mutant having a lethal mutation in the gene for the beta subunit of RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) exhibits an apparent 2- to 3-fold decrease in the rates of both beta and beta' subunit synthesis at the non-permissive temperature, relative to total protein. In contrast, a temperature-sensitive mutant with a lethal mutation in the gene encoding beta' has a 5- to 6-fold increase in the rates of beta and beta' synthesis at 42 degrees. These beta and beta' mutants also exhibit rapid degradation of both subunits at the high temperature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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