Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Jul;72(7):2640–2643. doi: 10.1073/pnas.72.7.2640

Minimal requirements for template recognition by bacteriophage Qβ replicase: Approach to general RNA-dependent RNA synthesis

Bernd Küppers 1, Manfred Sumper 1
PMCID: PMC432825  PMID: 1058479

Abstract

Any oligo- or polynucleotide able to offer a C-C-C-sequence at the 3′-terminus and a second C-C-C-sequence in a defined steric position to Qβ replicase is an efficient template. Corresponding chemical modifications convert non-template RNAs to template RNAs.

Keywords: protein-nucleic acid interaction, oligonucleotides, RNA replication

Full text

PDF
2640

Images in this article

2642Image
on p.2642

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banerjee A. K., Rensing U., August J. T. Replication of RNA viruses. X. Replication of a natural 6 s RNA by the Q-beta RNA polymerase. J Mol Biol. 1969 Oct 28;45(2):181–193. doi: 10.1016/0022-2836(69)90098-9. [DOI] [PubMed] [Google Scholar]
  2. Billeter M. A., Dahlberg J. E., Goodman H. M., Hindley J., Weissmann C. Sequence of the first 175 nucleotides from the 5' terminus of Qbeta RNA synthesized in vitro. Nature. 1969 Dec 13;224(5224):1083–1086. doi: 10.1038/2241083a0. [DOI] [PubMed] [Google Scholar]
  3. Blumenthal T., Landers T. A., Weber K. Bacteriophage Q replicase contains the protein biosynthesis elongation factors EF Tu and EF Ts. Proc Natl Acad Sci U S A. 1972 May;69(5):1313–1317. doi: 10.1073/pnas.69.5.1313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fedoroff N. V., Zinder N. D. Properties of the phage f2 replicase. I. Optimal conditions for replicase activity and analysis of the polynucleotide product synthesized in vitro. J Biol Chem. 1972 Jul 25;247(14):4577–4585. [PubMed] [Google Scholar]
  5. Franze de Fernandez M. T., Eoyang L., August J. T. Factor fraction required for the synthesis of bacteriophage Qbeta-RNA. Nature. 1968 Aug 10;219(5154):588–590. doi: 10.1038/219588a0. [DOI] [PubMed] [Google Scholar]
  6. Glynn I. M., Chappell J. B. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. doi: 10.1042/bj0900147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Haruna I., Spiegelman S. Specific template requirments of RNA replicases. Proc Natl Acad Sci U S A. 1965 Aug;54(2):579–587. doi: 10.1073/pnas.54.2.579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kamen R. A new method for the purification of Q RNA-dependent RNA polymerase. Biochim Biophys Acta. 1972 Feb 23;262(1):88–100. doi: 10.1016/0005-2787(72)90221-3. [DOI] [PubMed] [Google Scholar]
  9. Kamen R. Characterization of the subunits of Q-beta replicase. Nature. 1970 Nov 7;228(5271):527–533. doi: 10.1038/228527a0. [DOI] [PubMed] [Google Scholar]
  10. Kondo M., Gallerani R., Weissmann C. Subunit structure of Q-beta replicase. Nature. 1970 Nov 7;228(5271):525–527. doi: 10.1038/228525a0. [DOI] [PubMed] [Google Scholar]
  11. Letendre C. H., Singer M. F. Studies on primer-independent polynucleotide phosphorylase of Micrococcus luteus. Reaction with N-ethylmaleimide. J Biol Chem. 1974 Dec 10;249(23):7383–7389. [PubMed] [Google Scholar]
  12. Martin F. H., Uhlenbeck O. C., Doty P. Self-complementary oligoribonucleotides: adenylic acid-uridylic acid block copolymers. J Mol Biol. 1971 Apr 28;57(2):201–215. doi: 10.1016/0022-2836(71)90341-x. [DOI] [PubMed] [Google Scholar]
  13. Mills D. R., Kramer F. R., Spiegelman S. Complete nucleotide sequence of a replicating RNA molecule. Science. 1973 Jun 1;180(4089):916–927. doi: 10.1126/science.180.4089.916. [DOI] [PubMed] [Google Scholar]
  14. Rensing U., August J. T. The 3'-terminus and the replication of phage RNA. Nature. 1969 Nov 29;224(5222):853–856. doi: 10.1038/224853a0. [DOI] [PubMed] [Google Scholar]
  15. Sumper M., Luce R. Evidence for de novo production of self-replicating and environmentally adapted RNA structures by bacteriophage Qbeta replicase. Proc Natl Acad Sci U S A. 1975 Jan;72(1):162–166. doi: 10.1073/pnas.72.1.162. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wahba A. J., Miller M. J., Niveleau A., Landers T. A., Carmichael G. G., Weber K., Hawley D. A., Slobin L. I. Subunit I of G beta replicase and 30 S ribosomal protein S1 of Escherichia coli. Evidence for the identity of the two proteins. J Biol Chem. 1974 May 25;249(10):3314–3316. [PubMed] [Google Scholar]
  17. Weber H., Billeter M. A., Kahane S., Weissmann C., Hindley J., Porter A. Molecular basis for repressor activity of Q replicase. Nat New Biol. 1972 Jun 7;237(75):166–170. doi: 10.1038/newbio237166a0. [DOI] [PubMed] [Google Scholar]
  18. de Wachter R., Merregaert J., Vandenberghe A., Contreras R., Fiers W. Studies on the bacteriophage MS2. The untranslated 5'-terminal nucleotide sequence preceding the first cistron. Eur J Biochem. 1971 Oct 14;22(3):400–414. doi: 10.1111/j.1432-1033.1971.tb01558.x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES