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. 1975 Jul;72(7):2714–2718. doi: 10.1073/pnas.72.7.2714

New histones found in mature mammalian testes.

A Shires, M P Carpenter, R Chalkley
PMCID: PMC432841  PMID: 170615

Abstract

Two new histones have been found in sexually mature testes of several mammals. The new histones have been identified as a new F1 (TF1) and F2b (TF2b) on the basis of their behavior upon chemical fractionation procedures and electrophoresis at several urea concentrations. The new testis-specific histones are absent in very immature animals and in somatic tissues. However, by day 20, in the rat, the histone pattern is that of the adult, displaying nearly a full complement of TF1 and TF2b. The new histone complement evidently is characteristic of prespermatid germ cells. The degree of evolutionary variations in these histones and in other basic sperm proteins, as detected by change in electrophoretic mobility, appears to be much greater than that seen in somatic histones.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balhorn R., Balhorn M., Morris H. P., Chalkley R. Comparative high-resolution electrophoresis of tumor histones: variation in phosphorylation as a function of cell replication rate. Cancer Res. 1972 Aug;32(8):1775–1784. [PubMed] [Google Scholar]
  2. Balhorn R., Rieke W. O., Chalkley R. Rapid electrophoretic analysis for histone phosphorylation. A reinvestigation of phosphorylation of lysine-rich histone during rat liver regeneration. Biochemistry. 1971 Oct 12;10(21):3952–3959. doi: 10.1021/bi00797a024. [DOI] [PubMed] [Google Scholar]
  3. CLERMONT Y., PEREY B. Quantitative study of the cell population of the seminiferous tubules in immature rats. Am J Anat. 1957 Mar;100(2):241–267. doi: 10.1002/aja.1001000205. [DOI] [PubMed] [Google Scholar]
  4. Coelingh J. P., Monfoort C. H., Rozijn T. H., Leuven J. A., Schiphof R., Steyn-Parvé E. P., Braunitzer G., Schrank B., Ruhfus A. The complete amino acid sequence of the basic nuclear protein of bull spermatozoa. Biochim Biophys Acta. 1972 Nov 28;285(1):1–14. doi: 10.1016/0005-2795(72)90174-2. [DOI] [PubMed] [Google Scholar]
  5. Coelingh J. P., Rozijn T. H., Monfoort C. H. Isolation and partial characterization of a basic protein from bovine sperm heads. Biochim Biophys Acta. 1969;188(2):353–356. doi: 10.1016/0005-2795(69)90091-9. [DOI] [PubMed] [Google Scholar]
  6. Easton D., Chalkley R. High-resolution electrophoretic analysis of the histones from embryos and sperm of Arbacia punctulata. Exp Cell Res. 1972 Jun;72(2):502–508. doi: 10.1016/0014-4827(72)90020-1. [DOI] [PubMed] [Google Scholar]
  7. Ingles C. J., Dixon G. H. Phosphorylation of protamine during spermatogenesis in trout testis. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1011–1018. doi: 10.1073/pnas.58.3.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kistler W. S., Geroch M. E., Williams-Ashman H. G. Specific basic proteins from mammalian testes. Isolation and properties of small basic proteins from rat testes and epididymal spermatozoa. J Biol Chem. 1973 Jul 10;248(13):4532–4543. [PubMed] [Google Scholar]
  9. Kistler W. S., Noyes C., Heinrikson R. L. Partial structural analysis of a highly basic low molecular weight protein from rat testis. Biochem Biophys Res Commun. 1974 Mar 25;57(2):341–347. doi: 10.1016/0006-291x(74)90935-8. [DOI] [PubMed] [Google Scholar]
  10. Louie A. J., Dixon G. H. Kinetics of enzymatic modification of the protamines and a proposal for their binding to chromatin. J Biol Chem. 1972 Dec 25;247(24):7962–7968. [PubMed] [Google Scholar]
  11. Marushige Y., Marushige K. Haemodynamic and coronary vascular responses after beta-adrenoceptor blockade in the anaesthetised dog: a comparison of tolamolol with practolol and propranolol. Biochim Biophys Acta. 1974 Apr 10;340(4):498–508. [PubMed] [Google Scholar]
  12. Marushige Y., Marushige K. Transformation of sperm histone during formation and maturation of rat spermatozoa. J Biol Chem. 1975 Jan 10;250(1):39–45. [PubMed] [Google Scholar]
  13. McMaster-Kaye R., Kaye J. S. An electrophoretic analysis of the histones of the house cricket. Arch Biochem Biophys. 1973 Jun;156(2):426–436. doi: 10.1016/0003-9861(73)90291-9. [DOI] [PubMed] [Google Scholar]
  14. Oliver D., Sommer K. R., Panyim S., Spiker S., Chalkley R. A modified procedure for fractionating histones. Biochem J. 1972 Sep;129(2):349–353. doi: 10.1042/bj1290349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Panyim S., Bilek D., Chalkley R. An electrophoretic comparison of vertebrate histones. J Biol Chem. 1971 Jul 10;246(13):4206–4215. [PubMed] [Google Scholar]
  16. Subirana J. A. Nuclear proteins from a somatic and a germinal tissue of the echinoderm Holothuria tubulosa. Exp Cell Res. 1970 Dec;63(2):253–260. doi: 10.1016/0014-4827(70)90211-9. [DOI] [PubMed] [Google Scholar]
  17. Sung M. T., Dixon G. H. Modification of histones during spermiogenesis in trout: a molecular mechanism for altering histone binding to DNA. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1616–1623. doi: 10.1073/pnas.67.3.1616. [DOI] [PMC free article] [PubMed] [Google Scholar]

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