Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Jul;72(7):2765–2769. doi: 10.1073/pnas.72.7.2765

New chromosomal location for structural genes of ribosomal proteins.

R J Watson, J Parker, N P Fiil, J G Flaks, J D Friesen
PMCID: PMC432852  PMID: 1101266

Abstract

An Escherichia coli mutant, ts9, previously reported by Flaks et al. (Cold Spring Harbor Symp. Quant. Biol. 31, 623-631, 1966) to have an electrophoretically altered ribosomal protein, has been further characterized and the altered component has been identified as L7/L12. Although mutant ts9 is temperature sensitive for growth (rts-), the rts and L7/L12 mutations are genetically separable and are both located between argH and rif. The L7/L12 mutation (rpyL) maps very close to relC, mutants of which have a defect in the 50S ribosomal subunit. The gene order is argH-rts-(rpyL,relC)-rif. Protein synthesis directed by bacteriophage lambdacI857S7drifd18 in ultraviolet-irradiated cells indicates that L7/L12, As well as L1, L10, L11, and possibly L8 or L9 are coded by the phage DNA. Our results indicate that rpyL is the structural gene for L7/L12 and that this region of the E. coli chromosome contains a cluster of structural genes for ribosomal proteins.

Full text

PDF
2765

Images in this article

2766Image
on p.2766
2767Image
on p.2767
2768Image
on p.2768

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. H. Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B". Proc Natl Acad Sci U S A. 1946 May;32(5):120–128. doi: 10.1073/pnas.32.5.120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ballesta J. P., Vazquez D. Activities of ribosomal cores deprived of proteins L7, L10, L11 and L12. FEBS Lett. 1974 Nov 15;48(2):266–270. doi: 10.1016/0014-5793(74)80483-7. [DOI] [PubMed] [Google Scholar]
  3. Birge E. A., Kurland C. G. Reversion of a streptomycin-dependent strain of Escherichia coli. Mol Gen Genet. 1970;109(4):356–369. doi: 10.1007/BF00267704. [DOI] [PubMed] [Google Scholar]
  4. Bollen A., Faelen M., Lecocq J. P., Herzog A., Zengel J., Kahan L., Nomura M. The structural gene for the ribosomal protein S18 in Escherichia coli. I. Genetic studies on a mutant having an alteration in the protein S18. J Mol Biol. 1973 Jun 5;76(4):463–472. doi: 10.1016/0022-2836(73)90485-3. [DOI] [PubMed] [Google Scholar]
  5. Brot N., Marcel R., Cupp L., Weissbach H. The enzymatic acetylation of ribosomal bound protein L 12 . Arch Biochem Biophys. 1973 Apr;155(2):475–477. doi: 10.1016/0003-9861(73)90140-9. [DOI] [PubMed] [Google Scholar]
  6. Brot N., Weisbach H. The enzymatic acetylation of E. coli ribosomal protein L 12 . Biochem Biophys Res Commun. 1972 Nov 1;49(3):673–679. doi: 10.1016/0006-291x(72)90464-0. [DOI] [PubMed] [Google Scholar]
  7. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  8. DeWilde M., Michel F., Broman K. The structural gene for ribosomal protein S18 in Escherichia coli. III. Mapping outside the ribosomal protein gene cluster at minute 84 on the genome. Mol Gen Genet. 1974;133(4):329–333. doi: 10.1007/BF00332708. [DOI] [PubMed] [Google Scholar]
  9. Flaks J. G., Leboy P. S., Birge E. A., Kurland C. G. Mutations and genetics concerned with the ribosome. Cold Spring Harb Symp Quant Biol. 1966;31:623–631. doi: 10.1101/sqb.1966.031.01.081. [DOI] [PubMed] [Google Scholar]
  10. Friesen J. D., Fiil N. P., Parker J. M., Haseltine W. A. A new relaxed mutant of Escherichia coli with an altered 50S ribosomal subunit. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3465–3469. doi: 10.1073/pnas.71.9.3465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hamel E., Koka M., Nakamoto T. Requirement of an Escherichia coli 50 S ribosomal protein component for effective interaction of the ribosome with T and G factors and with guanosine triphosphate. J Biol Chem. 1972 Feb 10;247(3):805–814. [PubMed] [Google Scholar]
  12. Hardy S. J., Kurland C. G., Voynow P., Mora G. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry. 1969 Jul;8(7):2897–2905. doi: 10.1021/bi00835a031. [DOI] [PubMed] [Google Scholar]
  13. Haseltine W. A., Block R., Gilbert W., Weber K. MSI and MSII made on ribosome in idling step of protein synthesis. Nature. 1972 Aug 18;238(5364):381–384. doi: 10.1038/238381a0. [DOI] [PubMed] [Google Scholar]
  14. Helser T. L., Davies J. E., Dahlberg J. E. Mechanism of kasugamycin resistance in Escherichia coli. Nat New Biol. 1972 Jan 5;235(53):6–9. doi: 10.1038/newbio235006a0. [DOI] [PubMed] [Google Scholar]
  15. Jaskunas S. R., Lindahl L., Nomura M. Specialized transducing phages for ribosomal protein genes of Escherichia coli. Proc Natl Acad Sci U S A. 1975 Jan;72(1):6–10. doi: 10.1073/pnas.72.1.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kaltschmidt E., Wittmann H. G. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem. 1970 Aug;36(2):401–412. doi: 10.1016/0003-2697(70)90376-3. [DOI] [PubMed] [Google Scholar]
  17. Kaltschmidt E., Wittmann H. G. Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1276–1282. doi: 10.1073/pnas.67.3.1276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kirschbaum J. B., Konrad E. B. Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase. J Bacteriol. 1973 Nov;116(2):517–526. doi: 10.1128/jb.116.2.517-526.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kirschbaum J. B., Scaife J. Evidence for a lambda transducing phage carrying the genes for the beta and beta' subunits of Escherichia coli RNA polymerase. Mol Gen Genet. 1974;132(3):193–201. doi: 10.1007/BF00269392. [DOI] [PubMed] [Google Scholar]
  20. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  21. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Möller W., Castleman H., Terhorst C. P. Characterization of an acidic protein in 50 s ribosomes of E. coli. FEBS Lett. 1970 Jun 8;8(4):192–196. doi: 10.1016/0014-5793(70)80261-7. [DOI] [PubMed] [Google Scholar]
  23. Möller W., Groene A., Terhorst C., Amons R. 50-S ribosomal proteins. Purification and partial characterization of two acidic proteins, A 1 and A 2, isolated from 50-S ribosomes of Escherichia coli. Eur J Biochem. 1972 Jan 31;25(1):5–12. doi: 10.1111/j.1432-1033.1972.tb01660.x. [DOI] [PubMed] [Google Scholar]
  24. Reid M. S., Bieleski R. L. A simple apparatus for vertical flat-sheet polyacrylamide gel electrophoresis. Anal Biochem. 1968 Mar;22(3):374–381. doi: 10.1016/0003-2697(68)90278-9. [DOI] [PubMed] [Google Scholar]
  25. Signer E. R. Interaction of prophages at the att80 site with the chromosome of Escherichia coli. J Mol Biol. 1966 Jan;15(1):243–255. doi: 10.1016/s0022-2836(66)80224-3. [DOI] [PubMed] [Google Scholar]
  26. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Terhorst C., Möller W., Laursen R., Wittmann-Liebold B. Amino acid sequence of a 50 S ribosomal protein involved in both EFG and EFT dependent GTP-hydrolysis. FEBS Lett. 1972 Dec 15;28(3):325–328. doi: 10.1016/0014-5793(72)80742-7. [DOI] [PubMed] [Google Scholar]
  28. Traut R. R., Delius H., Ahmad-Zadeh C., Bickle T. A., Pearson P., Tissières A. Ribosomal proteins of E. Coli: stoichiometry and implications for ribosome structure. Cold Spring Harb Symp Quant Biol. 1969;34:25–38. doi: 10.1101/sqb.1969.034.01.007. [DOI] [PubMed] [Google Scholar]
  29. Wong K. P., Paradies H. H. Shape properties of proteins L7 and L12 from E. coli ribosomes. Biochem Biophys Res Commun. 1974 Nov 6;61(1):178–184. doi: 10.1016/0006-291x(74)90550-6. [DOI] [PubMed] [Google Scholar]
  30. Zimmermann R. A., Ikeya Y., Sparling P. F. Alteration of ribosomal protein S4 by mutation linked to kasugamycin-resistance in Escherichia coli. Proc Natl Acad Sci U S A. 1973 Jan;70(1):71–75. doi: 10.1073/pnas.70.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES