Abstract
At early times after infection of a recA derivative of Escherichia coli with lambdab221c126red270a42 phage, a low but significant proportion of intracellular lambda molecules show a novel junction. These junctions are also present, although in reduced numbers, in a lysate obtained at late times after infection of a recA+ host with lambdacIIcIII phage. Fine structure and denaturation mapping analyses showed that these junctions occur at homologous positions and that they are compatible with the occurrence of a cross-strand exchange between lambda DNA duplexes similar to the type proposed in most molecular models for genetic recombination. However, the results are also consistent with the structures expected if a replicating growing point undergoes branch migration.
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- Broker T. R., Lehman I. R. Branched DNA molecules: intermediates in T4 recombination. J Mol Biol. 1971 Aug 28;60(1):131–149. doi: 10.1016/0022-2836(71)90453-0. [DOI] [PubMed] [Google Scholar]
- Chattoraj D. K., Inman R. B. Origin and direction of replication of bacteriophage 186 DNA. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1768–1771. doi: 10.1073/pnas.70.6.1768. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Emmons S. W. Bacteriophage lambda derivatives carrying two copies of the cohesive end site. J Mol Biol. 1974 Mar 15;83(4):511–525. doi: 10.1016/0022-2836(74)90511-7. [DOI] [PubMed] [Google Scholar]
- Emmons S. W., Thomas J. O. Tandem genetic duplications in phage lambda. IV. The locations of spontaneously arising tandem duplications. J Mol Biol. 1975 Jan 15;91(2):147–152. doi: 10.1016/0022-2836(75)90155-2. [DOI] [PubMed] [Google Scholar]
- Hotchkiss R. D. Models of genetic recombination. Annu Rev Microbiol. 1974;28(0):445–468. doi: 10.1146/annurev.mi.28.100174.002305. [DOI] [PubMed] [Google Scholar]
- Matsumoto L., Kasamatsu H., Pikó L., Vinograd J. Mitochondrial DNA replication in sea urchin oocytes. J Cell Biol. 1974 Oct;63(1):146–159. doi: 10.1083/jcb.63.1.146. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meselson M. S., Radding C. M. A general model for genetic recombination. Proc Natl Acad Sci U S A. 1975 Jan;72(1):358–361. doi: 10.1073/pnas.72.1.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sigal N., Alberts B. Genetic recombination: the nature of a crossed strand-exchange between two homologous DNA molecules. J Mol Biol. 1972 Nov 28;71(3):789–793. doi: 10.1016/s0022-2836(72)80039-1. [DOI] [PubMed] [Google Scholar]
- Sobell H. M. Molecular mechanism for genetic recombination. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2483–2487. doi: 10.1073/pnas.69.9.2483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sobell H. M. Symmetry in protein-nucleic acid interaction and its genetic implications. Adv Genet. 1973;17:411–490. doi: 10.1016/s0065-2660(08)60175-3. [DOI] [PubMed] [Google Scholar]