Abstract
Rabbit globin poly(A)-free and native mRNA preparations were microinjected into Xenopus oocytes. The amount of globin message remaining after incubation of injected oocytes was determined by molecular hybridization with a radioactive complementary DNA probe, synthesized by copying globin mRNA with purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus. The results show that 56 hr after injection about 85% of the poly(A)-free mRNA molecules are degraded, while native poly(A)-containing mRNA chains are almost completely preserved during the same period of time.
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- Allende C. C., Allende J. E., Firtel R. A. The degradation of ribonucleic acids injected into Xenopus laevis oocytes. Cell. 1974 Jul;2(3):189–196. doi: 10.1016/0092-8674(74)90093-2. [DOI] [PubMed] [Google Scholar]
- Giglioni B., Gianni A. M., Comi P., Ottolenghi S., Rungger D. Translational control of globin synthesis by haemin in Xenopus oocytes. Nat New Biol. 1973 Nov 28;246(152):99–102. doi: 10.1038/newbio246099a0. [DOI] [PubMed] [Google Scholar]
- Gurdon J. B., Lingrel J. B., Marbaix G. Message stability in injected frog oocytes: long life of mammalian alpha and beta globin messages. J Mol Biol. 1973 Nov 5;80(3):539–551. doi: 10.1016/0022-2836(73)90421-x. [DOI] [PubMed] [Google Scholar]
- Huez G., Marbaix G., Hubert E., Leclercq M., Nudel U., Soreq H., Salomon R., Lebleu B., Revel M., Littauer U. Z. Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3143–3146. doi: 10.1073/pnas.71.8.3143. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kacian D. L., Watson K. F., Burny A., Spiegelman S. Purification of the DNA polymerase of avian myeloblastosis virus. Biochim Biophys Acta. 1971 Sep 24;246(3):365–383. doi: 10.1016/0005-2787(71)90773-8. [DOI] [PubMed] [Google Scholar]
- Soreq H., Nudel U., Salomon R., Revel M., Littauer U. Z. In vitro translation of polyadenylic acid-free rabbit globin messenger RNA. J Mol Biol. 1974 Sep 5;88(1):233–245. doi: 10.1016/0022-2836(74)90307-6. [DOI] [PubMed] [Google Scholar]
- Vogt V. M. Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae. Eur J Biochem. 1973 Feb 15;33(1):192–200. doi: 10.1111/j.1432-1033.1973.tb02669.x. [DOI] [PubMed] [Google Scholar]