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. 1975 Aug;72(8):3134–3138. doi: 10.1073/pnas.72.8.3134

Chromatin and DNA synthesis associated with nuclear membrane in germinating cotton.

W F Clay, F R Katterman, P G Bartels
PMCID: PMC432935  PMID: 1059099

Abstract

The synthesis of nuclear DNA and possible attachment sites of chromatin in the cells of cotton (Gossypium barbadense) radicles during germination was investigated. Biochemical analysis of nuclear membrane fragments or Sarkosyl-magnesium-membrane complexes indicates that the DNA, including newly replicated DNA, is attached to the nuclear membranes during periods of active synthesis. Electron micrographs of nuclear membrane fragments indicate a physical association between chromatin fibers and the membranes. The attachment site appears to be proteinaceous, since the chromatin is released by protein degradative enzymes as evidenced by biochemical techniques and electron microscopic observations. Short-term labeling results in incorporation into a membrane-associated product indistinguishable from the bulk of nuclear DNA. DNA polymerase activity is also associated with nuclear membrane preparations in which [3H]thymidine triphosphate is incorporated into an acid-insoluble. DNase-sensitive product.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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