Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1975 Aug;72(8):3215–3219. doi: 10.1073/pnas.72.8.3215

Structural similarities between a product of the T/t-locus isolated from sperm and teratoma cells, and H-2 antigens isolated from splenocytes.

E S Vitetta, K Artzt, D Bennett, E A Boyse, F Jacob
PMCID: PMC432953  PMID: 1059107

Abstract

Molecules specified by the H-2-linked T/t locus in the mouse are expressed on H-2-negative cells such as early embryos, sperm, and teratoma cells. By means of enzymatic radioiodination of cells and immunoprecipitation of lysates with congenic antiserum, one of these molecules, known as F9, has been obtained from sperm and teratoma cells and compared to H-2 isolated from murine splenocytes. Our studies indicate that both H-2 and F9 have identical molecular weights and subunit structure, including the presence of a B2-microglobulin-like subunit. These findings, taken together with previous studies of TL alloantigens, suggest that a number of gene products of the 9th linkage group show structural homology with each other and, in addition, with immunoglobulin. The genes in question may therefore have arisen from a primitive gene concerned with cellular recognition.

Full text

PDF
3215

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Artzt K., Bennett D., Jacob F. Primitive teratocarcinoma cells express a differentiation antigen specified by a gene at the T-locus in the mouse. Proc Natl Acad Sci U S A. 1974 Mar;71(3):811–814. doi: 10.1073/pnas.71.3.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Artzt K., Dubois P., Bennett D., Condamine H., Babinet C., Jacob F. Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2988–2992. doi: 10.1073/pnas.70.10.2988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Artzt K., Jacob F. Letter: Absence of serologically detectable H-2 on primitive teratocarcinoma cells in culture. Transplantation. 1974 Jun;17(6):632–634. doi: 10.1097/00007890-197406000-00015. [DOI] [PubMed] [Google Scholar]
  4. Baur S., Vitetta E. S., Sherr C. J., Schenkein I., Uhr J. W. Isolation of heavy and light chains of immunoglobulin from the surfaces of lymphoid cells. J Immunol. 1971 Apr;106(4):1133–1135. [PubMed] [Google Scholar]
  5. Bjaring B., Klein G. Antigenic characterization of heterozygous mouse lymphomas after immunoselection in vivo. J Natl Cancer Inst. 1968 Dec;41(6):1411–1429. [PubMed] [Google Scholar]
  6. Boyse E. A., Stockert E., Old L. J. Isoantigens of the H-2 and Tla loci of the mouse: interactions affecting their representation on thymocytes. J Exp Med. 1968 Jul 1;128(1):85–95. doi: 10.1084/jem.128.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Boyse E. A., Stockert E., Old L. J. Modification of the antigenic structure of the cell membrane by thymus-leukemia (TL) antibody. Proc Natl Acad Sci U S A. 1967 Sep;58(3):954–957. doi: 10.1073/pnas.58.3.954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cullen S. E., David C. S., Shreffler D. C., Nathenson S. G. Membrane molecules determined by the H-2 associated immune response region: isolation and some properties. Proc Natl Acad Sci U S A. 1974 Mar;71(3):648–652. doi: 10.1073/pnas.71.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cunningham B. A., Wang J. L., Berggård I., Peterson P. A. The complete amino acid sequence of beta 2-microglobulin. Biochemistry. 1973 Nov 20;12(24):4811–4822. doi: 10.1021/bi00748a001. [DOI] [PubMed] [Google Scholar]
  10. Heyner S., Brinster R. L., Palm J. Effect of iso-antibody on pre-implantation mouse embryos. Nature. 1969 May 24;222(5195):783–784. doi: 10.1038/222783a0. [DOI] [PubMed] [Google Scholar]
  11. LYON M. F., MEREDITH R. INVESTIGATIONS OF THE NATURE OF T-ALLELES IN THE MOUSE. II. GENETIC ANALYSIS OF AN UNUSUAL MUTANT ALLELE AND ITS DERIVATIVES. Heredity (Edinb) 1964 May;19:313–325. doi: 10.1038/hdy.1964.34. [DOI] [PubMed] [Google Scholar]
  12. Muramatsu T., Nathenson S. G., Boyse E. A., Old L. J. Some biochemical properties of thymus leukemia antigens solubilized from cell membranes by papain digestion. J Exp Med. 1973 May 1;137(5):1256–1262. doi: 10.1084/jem.137.5.1256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nicolas J. F., Dubois P., Jakob H., Gaillard J., Jacob F. Tératocarcinome de la souris: différenciation en culture d'une lignée de cellules primitives a potentialités multiples. Ann Microbiol (Paris) 1975 Jan;126(1):3–22. [PubMed] [Google Scholar]
  14. Old L. J., Stockert E., Boyse E. A., Kim J. H. Antigenic modulation. Loss of TL antigen from cells exposed to TL antibody. Study of the phenomenon in vitro. J Exp Med. 1968 Mar 1;127(3):523–539. doi: 10.1084/jem.127.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ostberg L., Rask L., Wigzell H., Peterson P. A. Thymus leukaemia antigen contains beta2-microglobulin. Nature. 1975 Feb 27;253(5494):735–737. doi: 10.1038/253735a0. [DOI] [PubMed] [Google Scholar]
  16. Palm J., Heyner S., Brinster R. L. Differential immunofluorescence of fertilized mouse eggs with H-2 and non-H-2 antibody. J Exp Med. 1971 Jun 1;133(6):1282–1293. doi: 10.1084/jem.133.6.1282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Peterson P. A., Cunningham B. A., Berggård I., Edelman G. M. 2 -Microglobulin--a free immunoglobulin domain. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1697–1701. doi: 10.1073/pnas.69.7.1697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rask L., Lindblom J. B., Peterson P. A. Subunit structure of H-2 alloantigens. Nature. 1974 Jun 28;249(460):833–834. doi: 10.1038/249833a0. [DOI] [PubMed] [Google Scholar]
  19. Rask L., Ostberg L., Lindblom B., Fernstedt Y., Peterson P. A. The subunit structure of transplantation antigens. Transplant Rev. 1974;21(0):85–105. doi: 10.1111/j.1600-065x.1974.tb01547.x. [DOI] [PubMed] [Google Scholar]
  20. Schwartz B. D., Nathenson S. G. Isolation of H-2 alloantigens solubilized by the detergent NP-40. J Immunol. 1971 Nov;107(5):1363–1367. [PubMed] [Google Scholar]
  21. Silver J., Hood L. Detergent-solubilised H-2 alloantigen is associated with a small molecular weight polypeptide. Nature. 1974 Jun 21;249(459):764–765. doi: 10.1038/249764a0. [DOI] [PubMed] [Google Scholar]
  22. Smithies O., Poulik M. D. Initiation of protein synthesis at an unusual position in an immunoglobulin gene? Science. 1972 Jan 14;175(4018):187–189. doi: 10.1126/science.175.4018.187. [DOI] [PubMed] [Google Scholar]
  23. Snell G. D. The H-2 locus of the mouse: observations and speculations concerning its comparative genetics and its polymorphism. Folia Biol (Praha) 1968;14(5):335–358. [PubMed] [Google Scholar]
  24. Strominger J. L., Cresswell P., Grey H., Humphreys R. E., Mann D., McCuneJ, Parham P., Robb R., Sanderson A. R., Springer T. A. The immunoglobulin-like structure of human histocompatibility antigens. Transplant Rev. 1974;21(0):126–143. doi: 10.1111/j.1600-065x.1974.tb01549.x. [DOI] [PubMed] [Google Scholar]
  25. Vitetta E. S., Melcher U., McWilliams M., Lamm M. E., Phillips-Quagliata J. M., Uhr J. W. Cell surface immunoglobulin. XI. The appearance of an IgD-like molecule on murine lymphoid cells during ontogeny. J Exp Med. 1975 Jan 1;141(1):206–215. doi: 10.1084/jem.141.1.206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Vitetta E. S., Uhr J. W., Boyse E. A. Association of a beta2-microglobulin-like subunit with H-2 and TL alloantigens on murine thymocytes. J Immunol. 1975 Jan;114(1 Pt 1):252–254. [PubMed] [Google Scholar]
  27. Vitetta E., Uhr J. W., Boyse E. A. Isolation and characterization of H-2 and TL alloantigens from the surface of mouse lymphocytes. Cell Immunol. 1972 Jun;4(2):187–191. doi: 10.1016/0008-8749(72)90019-6. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES