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. 2015 Feb 16;5:8488. doi: 10.1038/srep08488

Visualization of Endogenous and Exogenous Hydrogen Peroxide Using A Lysosome-Targetable Fluorescent Probe

Dabin Kim 1, Gyoungmi Kim 1, Sang-Jip Nam 1, Jun Yin 1,2,a, Juyoung Yoon 1,b
PMCID: PMC4329546  PMID: 25684681

Abstract

Reactive oxygen species (ROS) play crucial roles in diverse physiological processes; therefore, the efficient detection of ROS is very crucial. In this study, we report a boronate-based hydrogen peroxide (H2O2) probe having naphthalimide fluorophore. This probe also contained a morpholine moiety as a directing group for lysosome. The recognition property indicated that the probe exhibited high selectivity towards H2O2 not only in the solution but also in the living cells. Furthermore, it was used to monitor the level of endogenous and exogenous H2O2. These results support that the probe can function as an efficient indicator to detect H2O2.

Reactive oxygen species (ROS) are known to play key roles in the pathogenesis of serious diseases including cancer, neurodegenerative disorders, and cardiovascular diseases1,2,3. Therefore, efficient monitoring of the level of ROS in the living cells and tissues is very crucial for normal body functions and is helpful to estimate the biological processes2,3,4,5 During the metabolism, the body produces a number of species derived from oxygen mainly including superoxide (·O2−), hydroxyl radical (·OH), peroxyl radical (·OOR), single oxygen (1O2), hypochlorous acid/hypochlorite (HOCl/ClO−), and hydrogen peroxide (H2O2). Among them, H2O2 plays diverse physiological roles including host defense, immune responses, and cellular signal transduction6. Specifically, increased concentrations of H2O2 may cause oxidative damage of cellular proteins, ageing, and diseases7. Therefore, the detection of H2O2 in living cells has been developed as a popular area in biological and chemical researches8,9,10,11. Undoubtedly, the chemical probes based on the fluorescence signal transduction provide a visualization tool for H2O2 detection.

Boronate has been known to efficiently react with H2O2 under weak alkaline condition to generate phenol12. Furthermore, the negative charge on the phenolate is easy to transfer to carbamate, forming unprotected amine13. Therefore, p-dihydroxyborylbenzyloxycarbonyl moiety has become one of the most efficient reactive groups with H2O2. In recent decade, fluorescent probes have been widely employed with different fluorophores, which were successfully applied in various living cells and tissues for monitoring the H2O2-induced biological process14,15,16,17,18,19,20,21. However, detecting H2O2 in some special organelles such as in lysosome is still very challenging. In addition, ROS can yield reactive products that accumulate and contribute to aging and disease in the process of catalyzing metals of body with proteins. For instance, the accumulation of peroxidized lipids and proteins in lysosomes of the brain cells can result in Alzheimer disease22. Simultaneously, the dismutation of peroxy radical (·O2−) can generate H2O2, which is also a precursor of hypochlorous acid23. Therefore, the efficient monitoring and detection of H2O2 in lysosomes is significant in physiology and clinical diagnosis.

Toward this purpose, herein, we present a lysosome-targetable fluorescent probe based on naphthalimide fluorophore by using a popular p-dihydroxyborylbenzyloxycarbonyl moiety as a transponder. We investigated its optical properties and response toward various ROS and reactive nitrogen species. The result suggests that it possesses impressively high selectivity towards H2O2. The cell imaging confirmed that it can be used effectively as an indicator to monitor the level of H2O2 in lysosomes.

Results and Discussion

Design and synthesis

In usual, accurate method for the detection of species in special location of the cells is very crucial. In this case, we used morpholine an accepted directing group, which has been widely applied in the guidance of lysosomes24,25,26,27,28. In addition, p-dihydroxyborylbenzyloxycarbonyl has been proved to be an efficient moiety for the detection of H2O2. Similarly, it was also selected as a transponder in this study case. Therefore, morpholine and p-dihydroxyborylbenzyloxycarbonyl moieties were introduced into the naphthalimide, as shown in Figure 1.

Figure 1. The structure of lysosome-targetable H2O2 probe 1.

Figure 1

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The synthetic route of probe 1 is outlined in Figure 2. As shown, commercial bromide 2 as a starting material was treated with 2-morpholinoethanamine 3 in ethanol to afford the morpholine-modified naphthalimide bromide 4 in a high yield. Subsequent ammonization with piperazine generates the intermediate 6, which is treated with boronate 9 synthesized by the reaction of compounds 7 and triphosgene 8 to yield the probe 1. The structures of new intermediates and target molecule were well characterized by the standard spectroscopic techniques such as NMR spectroscopy and mass spectrometry (see the Supporting Information).

Figure 2. The synthesis of lysosome-targetable H2O2 probe 1.

Figure 2

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Optical properties

The photophysical properties of probe 1 (2 μM) were determined in phosphate-buffered saline (0.1 M PBS, pH 7.4, containing 1% DMF). The UV/vis absorption spectrum of probe 1 at 400 nm was recorded. A weak fluorescence signal with an emission maximum at 528 nm was induced at an excitation wavelength of 405 nm. Due to the introduction of boronate, subsequent investigation focused on the response of probe 1 toward various ROSs (200 μM). In the UV/vis absorption spectra, no significant changes were observed after incubation for 2 h with various ROSs such as H2O2, ·OH, ONOO−, ·OOR, ·NO, ClO−, and t-ROOH at 25°C (Figure S1 in the Supporting Information (SI)). However, an obvious increase in the fluorescence intensity was observed only when H2O2 was added under the same condition in comparison to other ROS, as shown in Figure 3(A), clearly indicating that probe 1 was highly selective for H2O2.

Figure 3.

Figure 3

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(A) Fluorescence spectra of probe 1 (2 μM) with various ROS (200 μM) and (B) Fluorescent titration of probe 1 (2 μM) upon addition of H2O2 (0–100 eq.) in PBS (pH 7.4) solution containing 1% DMF after incubation for 2 h at 25°C at an excitation wavelength of 405 nm, and excitation and emission slit widths of 3 and 5 mm, respectively.

Subsequently, the fluorescent titration of probe 1 (2 μM) upon addition of H2O2 (0–100 eq.) in PBS (pH 7.4) solution containing 1% DMF was investigated. As shown in Figure 3(B), the H2O2 induced an obvious fluorescence enhancement along with the increasing H2O2. These results strongly suggested that probe 1 was highly selective for the detection of H2O2 in aqueous solution. Next, we investigated the time-dependent UV/vis absorption and fluorescence spectra of probe 1 (2 μM) with H2O2 (200 μM) in PBS buffer solution (0.1 M PBS, pH 7.4, containing, 1% DMF). Similarly, no obvious changes were observed in the UV/vis absorption spectra (Figure S2 in the SI). However, a gradual fluorescence enhancement was observed (Figure S3 in the SI). As a comparison, other ROS could not induce a significant fluorescence change even after incubating with H2O2 over 2 h.

Bioimaging in living cells

As described above, probe 1 with high selectivity toward H2O2 and good water solubility permitted its further application in the living cells. In view of the fact that boronate-based fluorescence probes could function as an indicator for other ROSs such as ClO−, ONOO−, and peroxide except for H2O229,30,31,32,33, first, we investigated the selectivity of probe 1 in the living cells toward H2O2. HeLa cells were incubated with probe 1 (5 μM), and then incubated with ClO−, ONOO−, and H2O2 (100 μM), respectively. As shown in Figure S4, only H2O2 could induce an obvious red emission, which further confirmed that probe 1 had high selectivity not only in the solution but also in the living cells.

The good biocompatibility of authorized probe 1 was employed to assess the level of exogenous and endogenous H2O2 in the living cells. The HeLa cells were costained with probe 1 and LysoTracker Blue DND-22, which is a commercially available marker for lysosomes in Figure 4. In Figure 4A, the confocal microscopic image of probe 1 shows a red emission (Left). Along with the addition of 100 (Middle) and 200 μM (Right), an obvious fluorescence enhancement was observed. The result confirmed that probe 1 could monitor the level of H2O2 in the living cells. The imaging in Figures 4B and 4D strongly indicated that the probe possesses good cell-permeability and can be efficiently localized to lysosomes, as observed in other probes with a similar lysosome-targeting moiety24,25,26,27,28. Similar cell experiments were performed in living NIH-3T3 cells. The confocal microscopic image (Figure S5 in the SI) further confirmed that probe 1 is a lysosome-targetable H2O2 indicator. In view of H2O2 release from the cells and H2O2-induced biological processes, we used probe 1 to monitor the endogenous H2O2 in lysosome of the RAW 264.7 cells (Figure 5). As shown in Figure 5A (left), the confocal microscopic image of probe 1 shows a red emission owing to the existence of endogenous H2O2 released from the RAW 264.7 cells. For producing more endogenous H2O2, the RAW 264.7 cells were treated with PMA (Phorbol 12-myristate 13-acetate, 1 μg/mL). As might be expected, a significant fluorescence enhancement was observed (Figure 5A middle). Subsequent controllable experiments indicated that when TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl, 100 μM) was used as a ROS scavenger to block H2O2, decreasing in the fluorescence intensity (Figure 5A, right). These results in Figures 4 and 5 clearly indicate that lysosome-targetable probe 1 can be used as an efficient tool to monitor the level of exogenous and endogenous H2O2 in the living cells.

Figure 4. Probe 1 was localized to lysosomes in the HeLa cell.

Figure 4

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Confocal microscopic images of probe 1 on the exogenous H2O2: (A) Probe 1 (red, ex405/em490–590 nm); (B) LysoTracker Blue DND-22 (blue, ex405/em430–455 nm); (C) Bright field (gray); (D) Overlay of (A) and (B) (purple). Left: No treatment; Middle: probe 1 + 100 μM H2O2; Right: probe 1 + 200 μM H2O2. (All the cells were stained with 5 μM probe 1, 100 nM Lysotracker for 30 min and washed with DPBS and incubated with H2O2. Scale bar: 10 μm.).

Figure 5. Probe 1 was localized to lysosomes in RAW 264.7 cell.

Figure 5

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Confocal microscope images of probe 1 on the endogenous H2O2: (A) Probe 1 (red, ex405/em490–590 nm); (B) LysoTracker Blue DND-22 (blue, ex405/em430–455 nm); (C) Bright field (gray); (D) Overlay of (A) and (B) (purple). Left: No treatment; Middle: 1 μg/mL PMA (Phorbol 12-myristate 13-acetate), 1 h; Right: 1 μg/mL PMA, 1 h and 100 μM TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl, ROS scavenger), 1 h. (For producing endogenous H2O2, the RAW 264.7 cells were treated with PMA. All cells were stained with 5 μM probe 1, 100 nM Lysotracker for 30 min. Scale bar: 10 μm.).

Owing to its high selectivity and sensitivity, probe 1 was further subjected to explore the time-dependent fluorescence in the living cells. HeLa cells were incubated with 0 or 100 μM H2O2 for 30 min and then stained with probe 1 (0.2 μM). As shown in Figure 6A, the fluorescence image showed a weak red emission when the HeLa cells were not treated with H2O2. However, an increasing red emission was observed from the confocal microscopic images shown in Figure 6B when the HeLa cells were incubated with H2O2 (100 μM) as a result of the enhancement of fluorescence intensity as shown in Figure 6C, indicating that H2O2 could be easily captured by probe 1. The result further suggests that probe 1 is an efficient indicator for monitoring the level of H2O2 in the living cells.

Figure 6. Time-dependent fluorescence intensity of probe 1 in HeLa cells.

Figure 6

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HeLa cells were incubated with 0 or 100 μM H2O2 for 30 min and added 0.2 μM probe 1 for 30 min. The time-dependent fluorescence image of probe 1 was acquired by confocal microscopy: (A) no treatment; (B) 100 μM H2O2; (C) Time-dependent fluorescence intensity graph of probe 1 in HeLa cells. (Blue color: no treatment; Red color: 100 μM H2O2).

Reaction Mechanism

Understanding the sensor mechanism is very crucial for clarifying the optical properties. According to the similar reported mechanism2, we supposed that probe 1 was possibly involved in the reaction shown in Figure 1. To further demonstrate the recognition mechanism of probe 1 for H2O2, first, we checked the mass. Probe 1 showed a peak at 655.3 (m/z) before adding H2O2 (Figure S6 in the SI). A new peak at 395.2 (m/z) (Figure S7 in the SI) was assigned to the cleavable product 6 (Figure 1). Moreover, the HPLC of probe 1 and compound 6 showed two peaks at retention times of 8.62 and 6.00, respectively (Figure S8 in the SI). After addition of H2O2, the peak at 8.62 min disappeared along with the appearance of a new peak at 6.41 min, which further confirmed our hypothesis. Next, we investigated the time-dependent reaction between probe 1 and H2O2 by HPLC, which showed that the reaction finished in 90 min (Figure S9 in the SI). Despite the cleavable product 6 was found in the reaction of probe 1 and ClO− and ONOO−, respectively (Figure S10 and S11 in the SI), there was still much ROS in the system. These results further indicate that the probe 1 has high selectivity and sensitivity.

Consequently, a more detailed analysis of the structure and electron density was performed in an attempt to gain insight into its recognition mechanism. Accordingly, density functional theory (DFT) calculations were used to optimize the structures of probe 1 and compound 6 at the B3LYP/6-31G* level using a suite of Gaussian 09 programs. As shown in Figure 7, the piperazine ring in probe 1 and compound 6 showed a stable chair conformation, which was similar to our previous study34. For the probe 1, the electron density mainly focused on the morpholine unit in its HOMO orbital. In its excited state (LUMO orbital), the electron transfer occurs to the naphthalimide unit. The classic proton-induced electron transfer (PET) indicated that probe 1 had a weak fluorescence. While the cleavable product 6 was only involved in the part of electron transfer from piperazine ring to naphthalimide in Figure 7B, the main electron density focused on the naphthalimide moiety and piperazine units. Therefore, the cleavable product 6 was fluorescent. Moreover, the calculated HOMO−LUMO energy gap of probe 1 was 3.02 eV, which was lower in comparison to 6.

Figure 7. Frontier molecular orbital profiles of 1 and 6 based on DFT (B3LYP/6-31G*) calculations.

Figure 7

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Conclusion

In conclusion, we prepared a boronate-based hydrogen peroxide probe using naphthalimide as the fluorophore and morpholine moiety as the directing group, displaying high selectivity toward H2O2 not only in the solution but also in the living cells. The results of the cell imaging suggested that it can be used to efficiently monitor the level of endogenous and exogenous H2O2. Moreover, the time-dependent fluorescence bioimaging further confirmed that the probe can function as an efficient indicator for the detection of H2O2. This study provides an idea to monitor the changes of biological species in special cell tissues and may be helpful to promote its application in clinical medicine in the future.

Experimental

General Methods

All reactions and assembly processes were carried out under an argon atmosphere by using standard Schlenk techniques, unless otherwise stated. All starting materials and reagents were obtained commercially. 1H and 13C NMR spectra used CDCl3 solutions and a Bruker AM-300 spectrometer with tetramethylsilane (TMS) as the internal standard. Mass spectra were measured in the FAB mode. UV−vis spectra were obtained using a Scinco 3000 spectrophotometer (1 cm quartz cell) at 25°C. Fluorescence spectra were recorded on RF-5301/PC (Shimada) fluorescence spectrophotometer (1 cm quartz cell) at 25°C. Deionized water was used to prepare all aqueous solutions.

Synthesis of 4

4-(2-aminoethyl)-morpholine 3 (1.00 g, 7.68 mmol) was added to a solution of 4-bromo-1,8-naphthalic anhydride 2 (1.00 g, 3.61 mmol) in anhydrous CH3OH (20 mL) under nitrogen condition. The reaction mixture was refluxed for 8 hours and cooled down. After the residues was filtered, dried the yellowish filtered solid at room temperature in vacuum oven. Desired product was obtained as pale yellowish-white solid (yield 1.38 g, 98%). 1H NMR (CDCl3, 300 MHz): δ 8.65 (d,d, 1H), 8.57 (d,d, 1H), 8.40 (d, J = 8.1 Hz, 1H), 8.04 (d, J = 7.8 Hz, 1H), 7.85 (t, J = 7.2 Hz, 1H), 4.33(t, J = 6.9 Hz, 2H), 3.67 (t, J = 4.5 Hz, 4H), 2.70 (t, J = 7.2 Hz, 2H), 2.58 (t, J = 4.5 Hz, 4H). 13C NMR (CDCl3, 75 MHz) δ163.64, 163.62, 133.33, 132.05, 131.24, 131.13, 130.68, 130.34, 129.07, 128.10, 123.08, 122.21, 67.02, 67.02, 56.08, 53.81, 37.31. ESI-MS m/z = 389.0 [M + H]+, calcd for 389.0.

Synthesis of 6

Compounds 4 (0.60 g, 1.54 mmol) and piperazine anhydrous 5 (0.27 g, 3.08 mmol) were dissolved in methoxyl ethanol (10 mL) under nitrogen condition. The reaction mixture was refluxed for 3 hours and cooled down. Then the solvent was removed under reduced pressure. The residue was purified by silica gel column chromatography using CH2Cl2/MeOH (10:1, v:v) as the eluent. Desired product was obtained as yellow solid (yield 0.40 g, 66%). 1H NMR (CDCl3, 300 MHz): δ 8.57 (d of d, J = 7.2 Hz, 1H), 8.51 (d, J = 8.1 Hz, 1H), 8.42 (d of d, J = 8.4 Hz, 1H), 7.69 (d of d, J = 8.4 Hz, 1H), 7.21 (d, J = 8.1 Hz, 1H), 4.32(t, J = 6.9 Hz, 2H), 3.68 (t, J = 4.5 Hz, 4H), 3.23 (d, J = 5.7 Hz, 4H), 3.22 (d, J = 5.7 Hz, 4H), 2.69 (t, J = 6.9 Hz, 2H), 2.59 (t, J = 4.5 Hz, 4H). 13C NMR (CDCl3, 125 MHz) δ 164.75, 164.26, 156.69, 132.85, 131.43, 130.62, 130.20, 126.46, 125.87, 123.45, 116.75. 115.20, 67.28, 56.44, 54.65, 54.04, 47.07, 46.94, 46.46, 45.70, 41.48, 37.32. FAB-MS m/z = 395.21 [M + H]+, calcd for 395.21.

Synthesis of probe 1

Compounds 6 (0.19 g, 0.48 mmol) and 935 (0.10 g, 0.32 mmol) were dissolved in anhydride THF (10 mL) and refluxed overnight. After removing the solvent, the residue was purified by silica gel column chromatography using CH2Cl2/MeOH (10:1, v:v) as the eluent to afford pure product 1. Desired product was obtained as yellow solid (yield 0.10 g, 47%). 1H NMR (CDCl3, 300 MHz): δ 8.59 (d of d, J = 7.2 Hz, 1H), 8.52 (d, J = 8.1 Hz, 1H), 8.41 (d of d, J = 8.4 Hz, 1H), 7.82 (d, J = 8.1 Hz, 2H), 7.72 (d of d, J = 8.4 Hz, 1H), 7.39 (d, J = 8.1 Hz, 2H), 7.22 (d, J = 8.1 Hz, 1H), 5.20 (s, 2H), 4.33 (t, J = 6.9 Hz, 2H), 3.83 (s, 4H), 4.68 (t, J = 4.5 Hz, 4H), 3.22 (s, 4H), 2.69 (t, J = 6.9 Hz, 2H), 2.59 (s, 4H), 1.34 (s, 12H). 13C NMR (CDCl3, 75 MHz): δ 164.41, 155.49, 155.20, 139.44, 135.04, 132.46, 131.26, 129.87, 128.87, 127.21, 126.31, 126.07, 123.30, 117.39, 115.39, 83.90, 77.22, 67.39, 67.05, 56.19, 53.81, 52.88, 30.95, 24.86. FAB-MS m/z = 655.33 [M + H]+, calcd for 655.33.

Cell culture

HeLa cells (human epithelial adenocarcinoma), NIH-3T3 (mouse fibroblast) and RAW 264.7 cells (mouse macrophage cell) were obtained from Korean Cell Line Bank (Seoul, Korea). Cells were cultured in RPMI 1640 (Roswell Park Memorial Institute, without HEPES) supplemented with heat-inactivated 10% fetal bovine serum, 100 U/ml penicillin and 100 U/ml streptomycin. NIH- 3T3 cells were cultured in DMEM (Dulbecco's Modified Eagle's Medium) supplemented with heat-inactivated 10% bovine calf serum, 100 U/ml penicillin and 100 U/ml streptomycin. All cells were kept in 5% CO2 at 37°C.

Confocal microscopy imaging36,37,38

Cells were seeded in a 35-mm glass bottomed dishes at a density of 3 × 105 cells per dish in culture media. After 24 h, 5 μM probe 1 sensor and 100 nM Lysotracker Blue DND-22 added to the cells and the cells were incubated for 30 min at 37°C. For HeLa cells and NIH-3T3 cells, 100, 200 μM H2O2 added to the cell medium for 30 min and acquired images by confocal microscopy. For RAW 264.7 cells, 1 μg/ml PMA (phorbol 12-myristate 13-acetate) treated for 1 h and coincubated with 5 μM probe 1 for 30 min. For the inhibition test, 100 μM TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl) treated to the media for 1 h with PMA. After washing with the DPBS twice to remove the residual probe, the cells were imaged by confocal laser scanning microscopy (FV1200, Olympus, Japan). Cells were excited by a 405 nm diode laser and detected at BA 430–455 nm (Lysotracker), 490–590 nm (probe 1). For ROS selectivity in the cells, cells were incubated with 5 μM probe 1 for 30 min and washed with DPBS and incubated with 100 μM H2O2, OCl−, ONOO− for 30 min and acquired images by confocal microscopy.

Author Contributions

J.Yin and J.Yoon designed the experiments. D.K. synthesized and characterized probe 1, and generated the data shown in Fig. 1–6. G.K. performed the cell experiments. J.Yin carried out the DFT calculation. S.-J.N., J.Yin and J.Yoon wrote the manuscript.

Supplementary Material

Supplementary Information

Supporting Information

srep08488-s1.doc (5.1MB, doc)

Acknowledgments

This research was supported by a grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814). Jun Yin also acknowledges financial support from National Natural Science Foundation of China (21402057).

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