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. 1975 Sep;72(9):3347–3350. doi: 10.1073/pnas.72.9.3347

Identification of the 5' end of Rous sarcoma virus RNA.

J Keith, H Fraenkel-Conrat
PMCID: PMC432989  PMID: 171660

Abstract

The 5'-terminal triphosphate of the 35S RNA isolated from Rous sarcoma virus is blocked by 7-methylguanosine in 5' linkage with the penultimate nucleoside which is methylated in the 2'-O-ribose position, a type of endgroup found in all animal mRNAs investigated during the past year. The specific nuclease-resistant terminal fragment of RSV RNA has the structure 7mG5'ppp5'GmpCp-. This finding supports the belief that RNA of Rous sarcoma virus represents a (+) strand messenger which may be directly translated upon infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beemon K., Duesberg P., Vogt P. Evidence for crossing-over between avian tumor viruses based on analysis of viral RNAs. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4254–4258. doi: 10.1073/pnas.71.10.4254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Billeter M. A., Parsons J. T., Coffin J. M. The nucleotide sequence complexity of avian tumor virus RNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3560–3564. doi: 10.1073/pnas.71.9.3560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Both G. W., Banerjee A. K., Shatkin A. J. Methylation-dependent translation of viral messenger RNAs in vitro. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1189–1193. doi: 10.1073/pnas.72.3.1189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FRAENKEL-CONRAT H., SINGER B. The absence of phosphorylated chain ends in tobacco mosaic virus ribonucleic acid. Biochemistry. 1962 Jan;1:120–128. doi: 10.1021/bi00907a018. [DOI] [PubMed] [Google Scholar]
  5. Fraenkel-Conrat H., Fowlks E. Variability at the 5' ends of two plant viruses. Biochemistry. 1972 May 9;11(10):1733–1736. doi: 10.1021/bi00760a001. [DOI] [PubMed] [Google Scholar]
  6. Furuichi Y., Miura K. A blocked structure at the 5' terminus of mRNA from cytoplasmic polyhedrosis virus. Nature. 1975 Jan 31;253(5490):374–375. doi: 10.1038/253374a0. [DOI] [PubMed] [Google Scholar]
  7. Furuichi Y., Morgan M., Muthukrishnan S., Shatkin A. J. Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-. Proc Natl Acad Sci U S A. 1975 Jan;72(1):362–366. doi: 10.1073/pnas.72.1.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Furuichi Y., Muthukrishnan S., Shatkin A. J. 5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA. Proc Natl Acad Sci U S A. 1975 Feb;72(2):742–745. doi: 10.1073/pnas.72.2.742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Glitz D. G. The nucleotide sequence at the 3'-linked end of bacteriophage MS2 ribonucleic acid. Biochemistry. 1968 Mar;7(3):927–932. doi: 10.1021/bi00843a007. [DOI] [PubMed] [Google Scholar]
  10. Keith J., Gleason M., Fraenkel-Conrat H. Characterization of the end groups of RNA of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4371–4375. doi: 10.1073/pnas.71.11.4371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rapaport E., Zamecnik P. C. A new chemical procedure for 32P-labeling of ribonucleic acids at their 5'-ends after isolation. Proc Natl Acad Sci U S A. 1975 Jan;72(1):314–317. doi: 10.1073/pnas.72.1.314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rottman F., Heinlein K. Polynucleotides containing 2'-O-methyladenosine. I. Synthesis by polynucleotide phosphorylase. Biochemistry. 1968 Jul;7(7):2635–2641. doi: 10.1021/bi00847a028. [DOI] [PubMed] [Google Scholar]
  13. SINGER B., FRAENKEL-CONRAT H. THE NATURE OF THE BREAKS OCCURRING IN TMV-RNA UNDER VARIOUS CONDITIONS. Biochim Biophys Acta. 1963 Sep 17;76:143–145. [PubMed] [Google Scholar]
  14. SUGIYAMA T., FRAENKEL-CONRAT H. The end-groups of tobacco mosaic virus RNA. II. Nature of the 3'-linked chain end in TMV and of both ends in four strains. Biochemistry. 1963 Mar-Apr;2:332–334. doi: 10.1021/bi00902a026. [DOI] [PubMed] [Google Scholar]
  15. Shatkin A. J. Methylated messenger RNA synthesis in vitro by purified reovirus. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3204–3207. doi: 10.1073/pnas.71.8.3204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Silber R., Malathi V. G., Schulman L. H., Hurwitz J., Duesberg P. H. Studies of the Rous sarcoma virus RNA: characterization of the 5'-terminus. Biochem Biophys Res Commun. 1973 Jan 23;50(2):467–472. doi: 10.1016/0006-291x(73)90863-2. [DOI] [PubMed] [Google Scholar]
  17. Singer B. The chemical effects of nucleic acid alkylation and their relation to mutagenesis and carcinogenesis. Prog Nucleic Acid Res Mol Biol. 1975;15(0):219–284. [PubMed] [Google Scholar]
  18. Wei C. M., Moss B. Methylated nucleotides block 5'-terminus of vaccinia virus messenger RNA. Proc Natl Acad Sci U S A. 1975 Jan;72(1):318–322. doi: 10.1073/pnas.72.1.318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Young R. J., Content J. 5'-terminus of influenza virus RNA. Nat New Biol. 1971 Mar 31;230(13):140–142. doi: 10.1038/newbio230140a0. [DOI] [PubMed] [Google Scholar]
  20. von der Helm K., Duesberg P. H. Translation of Rous sarcoma virus RNA in a cell-free system from ascites Krebs II cells. Proc Natl Acad Sci U S A. 1975 Feb;72(2):614–618. doi: 10.1073/pnas.72.2.614. [DOI] [PMC free article] [PubMed] [Google Scholar]

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